دورية أكاديمية
أعرض في EDS

Decoding human in vitro terminal erythropoiesis originating from umbilical cord blood mononuclear cells and pluripotent stem cells.

التفاصيل البيبلوغرافية
العنوان: Decoding human in vitro terminal erythropoiesis originating from umbilical cord blood mononuclear cells and pluripotent stem cells.
المؤلفون: Wang X; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China., Zhang W; Beijing Institute of Genomics & China National Center for Bioinformation, Chinese Academy of Sciences, Beijing, PR China., Zhao S; Beijing Institute of Genomics & China National Center for Bioinformation, Chinese Academy of Sciences, Beijing, PR China., Yan H; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China., Xin Z; Beijing Institute of Genomics & China National Center for Bioinformation, Chinese Academy of Sciences, Beijing, PR China., Cui T; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China., Zang R; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China., Zhao L; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China., Wang H; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China., Zhou J; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China., Li X; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China., Yue W; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China., Xi J; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China., Zhang Z; Beijing Institute of Genomics & China National Center for Bioinformation, Chinese Academy of Sciences, Beijing, PR China.; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, PR China.; Sino-Danish College, University of Chinese Academy of Sciences, Beijing, PR China.; Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing, PR China., Fang X; Beijing Institute of Genomics & China National Center for Bioinformation, Chinese Academy of Sciences, Beijing, PR China.; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, PR China.; Sino-Danish College, University of Chinese Academy of Sciences, Beijing, PR China.; Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing, PR China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, PR China.; Savaid Medical School, University of Chinese Academy of Sciences, Beijing, PR China.; School of Future Technology, University of Chinese Academy of Sciences, Beijing, PR China., Pei X; Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine, Beijing, PR China.
المصدر: Cell proliferation [Cell Prolif] 2024 Jul; Vol. 57 (7), pp. e13614. Date of Electronic Publication: 2024 Mar 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Published for the Cell Kinetics Society, the European Study Group for Cell Proliferation, and the International Cell Cycle Society by Blackwell Scientific Publications Country of Publication: England NLM ID: 9105195 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2184 (Electronic) Linking ISSN: 09607722 NLM ISO Abbreviation: Cell Prolif Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Oxford, England] : Published for the Cell Kinetics Society, the European Study Group for Cell Proliferation, and the International Cell Cycle Society by Blackwell Scientific Publications, 1991-
مواضيع طبية MeSH: Erythropoiesis* , Pluripotent Stem Cells*/metabolism , Pluripotent Stem Cells*/cytology , Fetal Blood*/cytology , Fetal Blood*/metabolism , Leukocytes, Mononuclear*/metabolism , Leukocytes, Mononuclear*/cytology , Cell Differentiation*, Humans ; Cells, Cultured ; Cell Proliferation
مستخلص: Ex vivo red blood cell (RBC) production generates unsatisfactory erythroid cells. A deep exploration into terminally differentiated cells is required to understand the impairments for RBC generation and the underlying mechanisms. Here, we mapped an atlas of terminally differentiated cells from umbilical cord blood mononuclear cells (UCBMN) and pluripotent stem cells (PSC) and observed their dynamic regulation of erythropoiesis at single-cell resolution. Interestingly, we detected a few progenitor cells and non-erythroid cells from both origins. In PSC-derived erythropoiesis (PSCE), the expression of haemoglobin switch regulators (BCL11A and ZBTB7A) were significantly absent, which could be the restraint for its adult globin expression. We also found that PSCE were less active in stress erythropoiesis than in UCBMN-derived erythropoiesis (UCBE), and explored an agonist of stress erythropoiesis gene, TRIB3, could enhance the expression of adult globin in PSCE. Compared with UCBE, there was a lower expression of epigenetic-related proteins (e.g., CASPASE 3 and UBE2O) and transcription factors (e.g., FOXO3 and TAL1) in PSCE, which might restrict PSCE's enucleation. Moreover, we characterized a subpopulation with high proliferation capacity marked by CD99 high in colony-forming unit-erythroid cells. Inhibition of CD99 reduced the proliferation of PSC-derived cells and facilitated erythroid maturation. Furthermore, CD99-CD99 mediated the interaction between macrophages and erythroid cells, illustrating a mechanism by which macrophages participate in erythropoiesis. This study provided a reference for improving ex vivo RBC generation.
(© 2024 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.)
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معلومات مُعتمدة: 202002030025 Science and Technology Program of Guangzhou, China; 2017YFA0103100 National Key Research and Development Program of China; 2017YFA0103103 National Key Research and Development Program of China; 2017YFA0103104 National Key Research and Development Program of China; 2022YFC2406803 National Key Research and Development Program of China; XDA16010602 Strategic Priority Research Program of the Chinese Academy of Sciences; 81870097 National Natural Science Foundation of China; 82070114 National Natural Science Foundation of China; 82270126 National Natural Science Foundation of China; 32200589 National Natural Science Foundation of China; 82200690 National Natural Science Foundation of China; 32300612 National Natural Science Foundation of China
تواريخ الأحداث: Date Created: 20240318 Date Completed: 20240702 Latest Revision: 20240704
رمز التحديث: 20240704
مُعرف محوري في PubMed: PMC11216933
DOI: 10.1111/cpr.13614
PMID: 38499435
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-2184
DOI:10.1111/cpr.13614