دورية أكاديمية
Differential expression of mast cells in the small airways and alveolar septa of current smokers and patients with small airway disease and COPD.
| العنوان: | Differential expression of mast cells in the small airways and alveolar septa of current smokers and patients with small airway disease and COPD. |
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| المؤلفون: | Eapen MS; Respiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, Australia., Lu W; Respiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, Australia.; Launceston Respiratory and Sleep Centre, Launceston, Australia., Dey S; Respiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, Australia., Chia C; Respiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, Australia.; Launceston Respiratory and Sleep Centre, Launceston, Australia.; Department of Respiratory Medicine, Launceston General Hospital, Launceston, Australia., Hardikar A; Department of Cardiothoracic Surgery, Royal Hobart Hospital, Hobart, Australia., Hassan MI; Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India., Bhattarai P; Respiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, Australia., Gaikwad AV; Respiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, Australia., Das S; Faculty of Science, Centre for Inflammation, Centenary Institute and University of Technology Sydney School of Life Sciences, Sydney, Australia., Hansbro PM; Faculty of Science, Centre for Inflammation, Centenary Institute and University of Technology Sydney School of Life Sciences, Sydney, Australia., Singhera GK; Department of Anaesthesiology, Pharmacology and Therapeutics, University of British Columbia, Vancouver, BC, Canada.; Centre for Heart Lung Innovation, St Paul's Hospital, Vancouver, BC, Canada., Hackett TL; Department of Anaesthesiology, Pharmacology and Therapeutics, University of British Columbia, Vancouver, BC, Canada.; Centre for Heart Lung Innovation, St Paul's Hospital, Vancouver, BC, Canada., Sohal SS; Respiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, Australia.; Launceston Respiratory and Sleep Centre, Launceston, Australia. |
| المصدر: | ERJ open research [ERJ Open Res] 2024 Mar 18; Vol. 10 (2). Date of Electronic Publication: 2024 Mar 18 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: European Respiratory Society Country of Publication: England NLM ID: 101671641 Publication Model: eCollection Cited Medium: Print ISSN: 2312-0541 (Print) Linking ISSN: 23120541 NLM ISO Abbreviation: ERJ Open Res Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Sheffield : European Respiratory Society, [2015]- |
| مستخلص: | Background: COPD patients suffer from dysregulated and suppressed immune functionality, determined by their loss of degranulating capacity. Here we provide crucial information on the presence of degranulated mast cells (MCs) in COPD airways and demonstrate their relationship to lung physiology and airway remodelling. Methods: Small airway lung resections from non-smoking controls (NC), normal lung function smokers (NLFS), small airway disease (SAD), and mild-to-moderate COPD current smokers (COPD-CS) and ex-smokers (COPD-ES) were dual immuno-stained with MC tryptase and degranulation marker lysosome-associated membrane protein (LAMP)-1. Total MCs, degranulating MCs and non-MCs were enumerated in small airway epithelium and subepithelium, and in alveolar septa. Results: In the small airway wall subepithelial areas, COPD-CS and COPD-ES patients had significantly lower MCs than the NC group (p<0.05), although the numbers were considerably higher in the small airway epithelium (p<0.01). Degranulating non-MCs were higher in SAD (p<0.05) than in COPD in the small airway subepithelium. In contrast, there were significant increases in total MCs (degranulated and non-degranulated) and degranulated non-MCs in the alveolar septum of COPD patients compared with the NC group (p<001). The lower numbers of MCs in the subepithelium correlated with lower forced expiratory volume in 1 s (FEV Conclusions: This study is the first to assess the differential pattern of MC, degranulating MC and non-MC populations in the small airways and alveoli of COPD patients. The spatial positioning of the MCs within the airways showed variable correlations with lung function. Competing Interests: Conflict of interest: M.S. Eapen is currently a full-time employee in Mucpharm Pty Ltd; the work presented was not carried out as part of his employment with Mucpharm Pty Ltd. Conflict of interest: S.S. Sohal has served on the Small Airway Advisory Board for Chiesi Australia and has received the honorarium, outside the submitted work; reports travel support from Chiesi, GSK and AstraZeneca, outside the submitted work; reports a lecture honorarium from Chiesi, outside the submitted work; and has received a research grant from Lung Therapeutics, outside the submitted work. Conflict of interest: All the other authors do not have any conflict of interest to declare. (Copyright ©The authors 2024.) |
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| تواريخ الأحداث: | Date Created: 20240319 Latest Revision: 20240320 |
| رمز التحديث: | 20240320 |
| مُعرف محوري في PubMed: | PMC10945381 |
| DOI: | 10.1183/23120541.00579-2023 |
| PMID: | 38500797 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2312-0541 |
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| DOI: | 10.1183/23120541.00579-2023 |