دورية أكاديمية
Development of an ultrafast PCR to detect clinically relevant acquired vancomycin-resistance genes from cultured enterococci.
| العنوان: | Development of an ultrafast PCR to detect clinically relevant acquired vancomycin-resistance genes from cultured enterococci. |
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| المؤلفون: | Philip A; Team ReSIST, INSERM U1184, Faculty of Medicine Université Paris-Saclay, LabEx LERMIT, 78 rue du Général Leclerc, 94270 Le Kremlin-Bicêtre, France.; R&D, BforCure, 14 rue de la Beaune, 93100 Montreuil, France., Oueslati S; Team ReSIST, INSERM U1184, Faculty of Medicine Université Paris-Saclay, LabEx LERMIT, 78 rue du Général Leclerc, 94270 Le Kremlin-Bicêtre, France.; Bacteriology-Hygiene Department, Bicêtre Hospital, Assistance Publique/Hôpitaux de Paris, 94270 Le Kremlin-Bicêtre, France., Villa F; R&D, BforCure, 14 rue de la Beaune, 93100 Montreuil, France., Pannetier C; R&D, BforCure, 14 rue de la Beaune, 93100 Montreuil, France., Cattoir V; Department of Clinical Microbiology and French National Reference Centre for Antibiotic Resistance (Lab Enterococci), Rennes University Hospital, 35033 Rennes, France., Duranteau J; Surgical Intensive Care Department, Bicêtre Hospital, Assistance Publique-Hôpitaux de Paris, 94270 Le Kremlin-Bicêtre, France., Figueiredo S; Team ReSIST, INSERM U1184, Faculty of Medicine Université Paris-Saclay, LabEx LERMIT, 78 rue du Général Leclerc, 94270 Le Kremlin-Bicêtre, France.; Surgical Intensive Care Department, Bicêtre Hospital, Assistance Publique-Hôpitaux de Paris, 94270 Le Kremlin-Bicêtre, France., Naas T; Team ReSIST, INSERM U1184, Faculty of Medicine Université Paris-Saclay, LabEx LERMIT, 78 rue du Général Leclerc, 94270 Le Kremlin-Bicêtre, France.; Bacteriology-Hygiene Department, Bicêtre Hospital, Assistance Publique/Hôpitaux de Paris, 94270 Le Kremlin-Bicêtre, France. |
| المصدر: | The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2024 May 02; Vol. 79 (5), pp. 997-1005. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Validation Study; Evaluation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 7513617 Publication Model: Print Cited Medium: Internet ISSN: 1460-2091 (Electronic) Linking ISSN: 03057453 NLM ISO Abbreviation: J Antimicrob Chemother Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1997- : London : Oxford University Press Original Publication: London, New York, Academic Press. |
| مواضيع طبية MeSH: | Sensitivity and Specificity* , Real-Time Polymerase Chain Reaction*/methods , Vancomycin Resistance*/genetics , Vancomycin-Resistant Enterococci*/genetics , Vancomycin-Resistant Enterococci*/isolation & purification , Vancomycin-Resistant Enterococci*/drug effects, Humans ; Enterococcus/genetics ; Enterococcus/drug effects ; Enterococcus/isolation & purification ; Gram-Positive Bacterial Infections/microbiology ; Gram-Positive Bacterial Infections/diagnosis ; Bacterial Proteins/genetics ; Time Factors ; Genes, Bacterial/genetics |
| مستخلص: | Background: VRE are increasingly described worldwide. Screening of hospitalized patients at risk for VRE carriage is mandatory to control their dissemination. Here, we have developed the Bfast [VRE Panel] PCR kit, a rapid and reliable quantitative PCR assay for detection of vanA, vanB, vanD and vanM genes, from solid and liquid cultures adaptable to classical and ultrafast real-time PCR platforms. Methods: Validation was carried out on 133 well characterized bacterial strains, including 108 enterococci of which 64 were VRE. Analytical performances were determined on the CFX96 Touch (Bio-Rad) and Chronos Dx (BforCure), an ultrafast qPCR machine. Widely used culture plates and broths for enterococci selection/growth were tested. Results: All targeted van alleles (A, B, D and M) were correctly detected without cross-reactivity with other van genes (C, E, G, L and N) and no interference with the different routinely used culture media. A specificity and sensitivity of 100% and 99.7%, respectively, were determined, with limits of detection ranging from 21 to 238 cfu/reaction depending on the targets. The Bfast [VRE Panel] PCR kit worked equally well on the CFX and Chronos Dx platforms, with differences in multiplexing capacities (five and four optical channels, respectively) and in turnaround time (45 and 16 minutes, respectively). Conclusions: The Bfast [VRE Panel] PCR kit is robust, easy to use, rapid and easily implementable in clinical microbiology laboratories for ultra-rapid confirmation of the four main acquired van genes. Its features, especially on Chronos Dx, seem to be unmatched compared to other tools for screening of VRE. (© The Author(s) 2024. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| المشرفين على المادة: | 0 (Bacterial Proteins) |
| تواريخ الأحداث: | Date Created: 20240319 Date Completed: 20240502 Latest Revision: 20240724 |
| رمز التحديث: | 20240725 |
| DOI: | 10.1093/jac/dkae062 |
| PMID: | 38501366 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1460-2091 |
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| DOI: | 10.1093/jac/dkae062 |