دورية أكاديمية
Tracking Circulating HLA-Specific IgG-Producing Memory B Cells with the B-Cell ImmunoSpot Assay.
| العنوان: | Tracking Circulating HLA-Specific IgG-Producing Memory B Cells with the B-Cell ImmunoSpot Assay. |
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| المؤلفون: | Kervella D; Nephrology and Transplantation Laboratory, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain.; Nephrology and Kidney Transplantation Department, Vall d'Hebron University Hospital, Barcelona, Spain., Heidt S; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands., Fairchild R; Department of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA., Todryk S; Faculty of Health & Life Sciences, Northumbria University, Newcastle upon Tyne, UK. stephen.todryk@northumbria.ac.uk.; Translational & Clinical Research Institute, Newcastle University, Newcastle upon Tyne, UK. stephen.todryk@northumbria.ac.uk.; Cellular Technology Limited (CTL) Europe GmbH, Rutesheim, Germany. stephen.todryk@northumbria.ac.uk., Bestard O; Nephrology and Transplantation Laboratory, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain.; Nephrology and Kidney Transplantation Department, Vall d'Hebron University Hospital, Barcelona, Spain. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2768, pp. 201-209. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Isoantibodies* , Kidney Transplantation*, Humans ; Memory B Cells ; Graft Rejection ; HLA Antigens ; Histocompatibility Antigens Class II ; Immunoglobulin G ; Tissue Donors ; Graft Survival |
| مستخلص: | Donor-specific antibodies (DSA) against human leukocyte antigen (HLA) molecules are a major risk factor for rejection of transplanted organs (in antibody-mediated rejection [ABMR]), particularly in patients who have prior sensitization or receive insufficient immunosuppression through minimization or noncompliance. These DSA are measured routinely in the serum of patients prior to transplantation mainly using bead-based technologies or cell-based assays. However, the absence of detectable serum DSA does not always reflect the absence of sensitization or histologically defined ABMR, and so it has been proposed that the detection and measurement of memory B cells capable of secreting antibodies against donor HLA antigens could be carried out using B-cell ImmunoSpot, to better inform the degree of immune sensitization of transplant patients prior to as well as after transplantation. Such an assay is described here. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| References: | Bestard O, Thaunat O, Bellini MI et al (2022) Alloimmune risk stratification for kidney transplant rejection. Transpl Int 35:10138. (PMID: 10.3389/ti.2022.10138356699729163827) Meneghini M, Crespo E, Niemann M et al (2021) Donor/recipient HLA molecular mismatch scores predict primary humoral and cellular alloimmunity in kidney transplantation. Front Immunol 11:623276. (PMID: 10.3389/fimmu.2020.623276337769887988214) Lefaucheur C, Viglietti D, Bentlejewski C et al (2016) IgG donor-specific anti-human HLA antibody subclasses and kidney allograft antibody-mediated injury. J Am Soc Nephrol 27(1):293–304. (PMID: 10.1681/ASN.201411112026293822) Gorbacheva V, Fan R, Beavers A et al (2019) Anti-donor MHC Class II alloantibody induces glomerular injury in mouse renal allografts subjected to prolonged cold ischemia. Am Soc Nephrol 30(12):2413–2425. (PMID: 10.1681/ASN.2018111169) Shiu KY, Stringer D, McLaughlin L et al (2020) Effect of optimized immunosuppression (including rituximab) on anti-donor alloresponses in patients with chronically rejecting renal allografts. Front Immunol 11:79. (PMID: 10.3389/fimmu.2020.00079321172427012933) Heidt S, Roelen DL, de Vaal YJ et al (2012) A novel ELISPOT assay to quantify HLA-specific B cells in HLA-immunized individuals. Am J Transplant 12(6):1469–1478. (PMID: 10.1111/j.1600-6143.2011.03982.x22390272) Lúcia M, Luque S, Crespo E et al (2015) Preformed circulating HLA-specific memory B cells predict high risk of humoral rejection in kidney transplantation. Kidney Int 88(4):874–887. (PMID: 10.1038/ki.2015.20526176829) Karahan GE, de Vaal YJ, Roelen DL et al (2015) Quantification of HLA class II-specific memory B cells in HLA-sensitized individuals. Hum Immunol 76(2–3):129–136. (PMID: 10.1016/j.humimm.2015.01.01425636565) Karahan GE, de Vaal YJH, Krop J et al (2017) A memory B cell crossmatch assay for quantification of donor-specific memory B cells in the peripheral blood of HLA-immunized individuals. Am J Transplant 17(10):2617–2626. (PMID: 10.1111/ajt.1429328371365) Luque S, Lúcia M, Crespo E et al (2018) A multicolour HLA-specific B-cell FluoroSpot assay to functionally track circulating HLA-specific memory B cells. J Immunol Methods 462:23–33. (PMID: 10.1016/j.jim.2018.07.01130075182) Luque S, Lúcia M, Melilli E et al (2019) Value of monitoring circulating donor-reactive memory B cells to characterize antibody-mediated rejection after kidney transplantation. Am J Transplant 19(2):368–380. (PMID: 10.1111/ajt.1505530085394) Burton H, McLaughlin L, Shiu KY et al (2022) The phenotype of HLA-binding B cells from sensitized kidney transplant recipients correlates with clinically prognostic patterns of interferon-γ production against purified HLA proteins. Kidney Int 102(2):355–369. (PMID: 10.1016/j.kint.2022.02.04135483526) Sellarés J, de Freitas DG, Mengel M et al (2012) Understanding the causes of kidney transplant failure: the dominant role of antibody-mediated rejection and nonadherence. Am J Transplant 12(2):388–399. (PMID: 10.1111/j.1600-6143.2011.03840.x22081892) Stringer D, Gardner L, Shaw O et al (2023) Optimized immunosuppression to prevent graft failure in renal transplant recipients with HLA antibodies (OuTSMART): a randomised controlled trial. eClinicalMedicine 56. Available from: https://www.thelancet.com/journals/eclinm/article/PIIS2589-5370 (22)00548-X/fulltext. [cited 2023 May 9]. Bestard O, Couzi L, Crespo M et al (2021) Stratifying the humoral risk of candidates to a solid organ transplantation: a proposal of the ENGAGE working group. Transpl Int 34(6):1005–1018. (PMID: 10.1111/tri.1387433786891) Tambur AR, Bestard O, Campbell P et al (2023) Sensitization in transplantation: assessment of Risk 2022 working group meeting report. Am J Transplant 23(1):133–149. (PMID: 10.1016/j.ajt.2022.11.00936695615) Schinstock CA, Askar M, Bagnasco SM et al (2021) A 2020 Banff Antibody-mediated Injury Working Group examination of international practices for diagnosing antibody-mediated rejection in kidney transplantation – a cohort study. Transpl Int 34(3):488–498. (PMID: 10.1111/tri.1381333423340) Tarlinton D, Good-Jacobson K (2013) Diversity among memory B cells: origin, consequences, and utility. Science 341(6151):1205–1211. (PMID: 10.1126/science.124114624031013) |
| فهرسة مساهمة: | Keywords: B cells; HLA; ImmunoSpot; Transplantation; memory |
| المشرفين على المادة: | 0 (Isoantibodies) 0 (HLA Antigens) 0 (Histocompatibility Antigens Class II) 0 (Immunoglobulin G) |
| تواريخ الأحداث: | Date Created: 20240319 Date Completed: 20240321 Latest Revision: 20240321 |
| رمز التحديث: | 20240321 |
| DOI: | 10.1007/978-1-0716-3690-9_12 |
| PMID: | 38502395 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-3690-9_12 |