دورية أكاديمية
The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1.
| العنوان: | The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1. |
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| المؤلفون: | Bischof L; Universität Osnabrück, Fachbereich Biologie/Chemie, AG Genetik, Barbarastr. 11, Osnabrück D-49076, Germany., Schweitzer F; Universität Osnabrück, Fachbereich Biologie/Chemie, AG Genetik, Barbarastr. 11, Osnabrück D-49076, Germany., Schmitz HP; Universität Osnabrück, Fachbereich Biologie/Chemie, AG Genetik, Barbarastr. 11, Osnabrück D-49076, Germany., Heinisch JJ; Universität Osnabrück, Fachbereich Biologie/Chemie, AG Genetik, Barbarastr. 11, Osnabrück D-49076, Germany. Electronic address: jheinisc@uos.de. |
| المصدر: | European journal of cell biology [Eur J Cell Biol] 2024 Jun; Vol. 103 (2), pp. 151405. Date of Electronic Publication: 2024 Mar 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Germany NLM ID: 7906240 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-1298 (Electronic) Linking ISSN: 01719335 NLM ISO Abbreviation: Eur J Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jena, Germany : Elsevier Original Publication: Stuttgart : Wissenschaftliche Verlagsgesellschaft, <1979-1997> |
| مواضيع طبية MeSH: | Saccharomyces cerevisiae Proteins*/metabolism , Saccharomyces cerevisiae Proteins*/genetics , Oxidative Stress* , Saccharomyces cerevisiae*/metabolism , Saccharomyces cerevisiae*/genetics , Mitochondrial Membranes*/metabolism , rho GTP-Binding Proteins*/metabolism , rho GTP-Binding Proteins*/genetics, Protein Transport ; Voltage-Dependent Anion Channels/metabolism ; Voltage-Dependent Anion Channels/genetics ; Mitochondria/metabolism ; Mitochondrial Proteins/metabolism ; Mitochondrial Proteins/genetics ; Mitophagy ; Porins |
| مستخلص: | Yeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GDP/GTP exchange factor (GEF), remained unaffected. An influence of the voltage-dependent anion channel (VDAC) Por1 on the association of GFP-Rho5 with mitochondria under oxidative stress conditions appeared to be strain-dependent. However, epistasis analyses and bimolecular fluorescence complementation (BiFC) studies indicate a genetic and physical interaction. All four strains lacking a single MOMP were investigated for their effect on mitophagy. Competing Interests: Declaration of Competing Interest The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier GmbH.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Rho-type GTPase; glucose starvation; mitochondria; oxidative stress; porin |
| المشرفين على المادة: | 0 (Saccharomyces cerevisiae Proteins) EC 3.6.5.2 (rho GTP-Binding Proteins) 0 (Por1 protein, S cerevisiae) EC 3.6.5.2 (RHO5 protein, S cerevisiae) 0 (Voltage-Dependent Anion Channels) 0 (Mitochondrial Proteins) 0 (Porins) |
| تواريخ الأحداث: | Date Created: 20240319 Date Completed: 20240627 Latest Revision: 20240719 |
| رمز التحديث: | 20240719 |
| DOI: | 10.1016/j.ejcb.2024.151405 |
| PMID: | 38503132 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1618-1298 |
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| DOI: | 10.1016/j.ejcb.2024.151405 |