دورية أكاديمية
Co-infecting viruses of species Bovine rhinitis B virus (Picornaviridae) and Bovine nidovirus 1 (Tobaniviridae) identified for the first time from a post-mortem respiratory sample of a sheep (Ovis aries) in Hungary.
| العنوان: | Co-infecting viruses of species Bovine rhinitis B virus (Picornaviridae) and Bovine nidovirus 1 (Tobaniviridae) identified for the first time from a post-mortem respiratory sample of a sheep (Ovis aries) in Hungary. |
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| المؤلفون: | Tóth F; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary., Gáspár G; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary., Pankovics P; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary., Urbán P; János Szentágothai Research Centre of the University of Pécs, Bioinformatics Research Group, Genomics and Bioinformatics Core Facility, Pécs, Hungary., Herczeg R; János Szentágothai Research Centre of the University of Pécs, Bioinformatics Research Group, Genomics and Bioinformatics Core Facility, Pécs, Hungary., Albert M; Ceva Phylaxia Ltd. Budapest, Hungary., Reuter G; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary., Boros Á; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary. Electronic address: boros.akos@pte.hu. |
| المصدر: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2024 Jun; Vol. 120, pp. 105585. Date of Electronic Publication: 2024 Mar 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 101084138 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1567-7257 (Electronic) Linking ISSN: 15671348 NLM ISO Abbreviation: Infect Genet Evol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Picornaviridae*/genetics , Picornaviridae*/isolation & purification , Picornaviridae*/classification , Phylogeny* , Sheep Diseases*/virology , Picornaviridae Infections*/veterinary , Picornaviridae Infections*/virology, Animals ; Hungary ; Sheep ; Cattle ; Coinfection/virology ; Coinfection/veterinary ; Genome, Viral ; Nidovirales/genetics ; Nidovirales/isolation & purification ; Nidovirales/classification ; Nidovirales Infections/veterinary ; Nidovirales Infections/virology |
| مستخلص: | In this study, a picornavirus and a nidovirus were identified from a single available nasopharyngeal swab (NPS) sample of a freshly deceased sheep, as the only vertebrate viruses found with viral metagenomics and next-generation sequencing methods. The sample was originated from a mixed feedlot farm in Hungary where sheep and cattle were held together but in separate stalls. Most of the sheep had respiratory signs (coughing and increased respiratory effort) at the time of sampling. Other NPS were not, but additional enteric samples were collected from sheep (n = 27) and cattle (n = 11) of the same farm at that time. The complete/nearly complete genomes of the identified viruses were determined using RT-PCR and Nanopore (MinION-Flonge) / Dye-terminator sequencing techniques. The results of detailed genomic and phylogenetic analyses indicate that the identified picornavirus most likely belongs to a type 4 genotype of species Bovine rhinitis B virus (BRBV-4, OR885914) of genus Aphthovirus, family Picornaviridae while the ovine nidovirus (OvNV, OR885915) - as a novel variant - could belong to the recently created Bovine nidovirus 1 (BoNV) species of genus Bostovirus, family Tobaniviridae. None of the identified viruses were detectable in the enteric samples using RT-PCR and generic screening primer pairs. Both viruses are well-known respiratory pathogens of cattle, but their presence was not demonstrated before in other animals, like sheep. Furthermore, neither BRBV-4 nor BoNVs were investigated in European cattle and/or sheep flocks, therefore it cannot be determined whether the presence of these viruses in sheep was a result of a single host species switch/spillover event or these viruses are circulating in not just cattle but sheep populations as well. Further studies required to investigate the spread of these viruses in Hungarian and European sheep and cattle populations and to identify their pathogenic potential in sheep. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Bovine rhinitis B virus; Co-infection; Nidovirus; Picornavirus; Respiratory disease; Sheep; Spillover |
| تواريخ الأحداث: | Date Created: 20240320 Date Completed: 20240424 Latest Revision: 20240424 |
| رمز التحديث: | 20240425 |
| DOI: | 10.1016/j.meegid.2024.105585 |
| PMID: | 38508364 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1567-7257 |
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| DOI: | 10.1016/j.meegid.2024.105585 |