دورية أكاديمية
Broad host range phages target global Clostridium perfringens bacterial strains and clear infection in five-strain model systems.
| العنوان: | Broad host range phages target global Clostridium perfringens bacterial strains and clear infection in five-strain model systems. |
|---|---|
| المؤلفون: | Thanki AM; Department of Genetics and Genome Biology, Leicester Centre for Phage Research, University of Leicester, Leicester, United Kingdom., Osei EK; Department of Agriculture, Food and the Marine, Teagasc Food Research Centre, Moorepark, Ireland.; APC Microbiome Ireland, University College, Cork, Ireland., Whenham N; AB Agri, Innovation Way, Peterborough Business Park, Peterborough, United Kingdom., Salter MG; AB Agri, Innovation Way, Peterborough Business Park, Peterborough, United Kingdom., Bedford MR; AB Vista, Woodstock Court, Marlborough Business Park, Marlborough, Wiltshire, United Kingdom., Masey O'Neill HV; AB Agri, Innovation Way, Peterborough Business Park, Peterborough, United Kingdom., Clokie MRJ; Department of Genetics and Genome Biology, Leicester Centre for Phage Research, University of Leicester, Leicester, United Kingdom. |
| المصدر: | Microbiology spectrum [Microbiol Spectr] 2024 May 02; Vol. 12 (5), pp. e0378423. Date of Electronic Publication: 2024 Mar 21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : ASM Press, 2013- |
| مواضيع طبية MeSH: | Clostridium perfringens*/virology , Bacteriophages*/physiology , Clostridium Infections*/microbiology , Clostridium Infections*/therapy , Clostridium Infections*/veterinary , Host Specificity* , Poultry Diseases*/microbiology , Poultry Diseases*/virology, Animals ; Virulence ; Chickens ; Poultry/microbiology ; Phage Therapy/methods ; Larva/microbiology ; Larva/virology ; Disease Models, Animal |
| مستخلص: | Clostridium perfringens is a prevalent bacterial pathogen in poultry, and due to the spread of antimicrobial resistance, alternative treatments are needed to prevent and treat infection. Bacteriophages (phages), viruses that kill bacteria, offer a viable option and can be used therapeutically to treat C. perfringens infections. The aim of this study was to isolate phages against C. perfringens strains currently circulating on farms across the world and establish their virulence and development potential using host range screening, virulence assays, and larva infection studies. We isolated 32 phages of which 19 lysed 80%-92% of our global C. perfringens poultry strain collection ( n = 97). The virulence of these individual phages and 32 different phage combinations was quantified in liquid culture at multiple doses. We then developed a multi-strain C. perfringens larva infection model, to mimic an effective poultry model used by the industry. We tested the efficacy of 16/32 phage cocktails in the larva model. From this, we identified that our phage cocktail consisting of phages CPLM2, CPLM15, and CPLS41 was the most effective at reducing C. perfringens colonization in infected larvae when administered before bacterial challenge. These data suggest that phages do have significant potential to prevent and treat C. perfringens infection in poultry. Importance: Clostridium perfringens causes foodborne illness worldwide, and 95% of human infections are linked to the consumption of contaminated meat, including chicken products. In poultry, C. perfringens infection causes necrotic enteritis, and associated mortality rates can be up to 50%. However, treating infections is difficult as the bacterium is becoming antibiotic-resistant. Furthermore, the poultry industry is striving toward reduced antibiotic usage. Bacteriophages (phages) offer a promising alternative, and to progress this approach, robust suitable phages and laboratory models that mimic C. perfringens infections in poultry are required. In our study, we isolated phages targeting C. perfringens and found that many lyse C. perfringens strains isolated from chickens worldwide. Consistent with other published studies, in the model systems we assayed here, when some phages were combined as cocktails, the infection was cleared most effectively compared to individual phage use. Competing Interests: The research was funded by Ab Agri, the global feed manufacturing company. They were not involved in designing experiments and data analysis. This will all be Anisha M. Thanki and Martha R.J. Clokie at the University of Leicester. |
| References: | Emerg Microbes Infect. 2018 Aug 6;7(1):141. (PMID: 30082713) Poult Sci. 2022 Feb;101(2):101590. (PMID: 34953377) Emerg Microbes Infect. 2023 Dec;12(1):2217947. (PMID: 37224439) Front Microbiol. 2016 Aug 31;7:1383. (PMID: 27630633) Virulence. 2016 Apr 2;7(3):214-29. (PMID: 26730990) Microbiol Spectr. 2022 Jun 29;10(3):e0042222. (PMID: 35579475) Front Vet Sci. 2022 Dec 22;9:1058115. (PMID: 36619945) Front Microbiol. 2021 Jan 21;11:609955. (PMID: 33552020) Anaerobe. 2023 Jun;81:102736. (PMID: 37196842) Pharmaceuticals (Basel). 2022 Jan 02;15(1):. (PMID: 35056115) Int J Food Microbiol. 2022 Jan 16;361:109446. (PMID: 34742146) Eur J Pharm Biopharm. 2018 Jun;127:213-222. (PMID: 29486303) Pathogens. 2023 May 26;12(6):. (PMID: 37375458) Front Microbiol. 2019 Jul 02;10:1491. (PMID: 31312191) Parasitol Res. 2003 May;90(1):19-26. (PMID: 12743800) Sci Rep. 2022 Feb 14;12(1):2458. (PMID: 35165352) Sci Rep. 2022 Jan 19;12(1):991. (PMID: 35046416) Br Poult Sci. 2017 Jun;58(3):290-297. (PMID: 28277796) Biology (Basel). 2022 Apr 01;11(4):. (PMID: 35453750) Methods Mol Biol. 2009;501:141-9. (PMID: 19066818) Viruses. 2022 Mar 25;14(4):. (PMID: 35458406) Front Microbiol. 2021 Mar 29;12:613529. (PMID: 33868187) Sci Rep. 2017 Jun 1;7(1):2614. (PMID: 28572684) Animals (Basel). 2020 Sep 22;10(9):. (PMID: 32972009) Microb Pathog. 2023 Oct;183:106279. (PMID: 37549798) Int J Mol Sci. 2023 Feb 20;24(4):. (PMID: 36835606) Methods Mol Biol. 2009;501:69-76. (PMID: 19066811) PeerJ. 2014 Sep 25;2:e590. (PMID: 25279269) Phage (New Rochelle). 2020 Mar 1;1(1):27-36. (PMID: 36147620) Avian Dis. 2010 Mar;54(1):33-40. (PMID: 20408396) J Anim Sci Biotechnol. 2022 Dec 14;13(1):148. (PMID: 36514172) |
| معلومات مُعتمدة: | Ab Agri; Commonwealth Scholarship Commission (CSC) |
| فهرسة مساهمة: | Keywords: Clostridium perfringens; Galleria mellonella; bacteriophage therapy; bacteriophages; phage cocktails |
| تواريخ الأحداث: | Date Created: 20240321 Date Completed: 20240502 Latest Revision: 20240511 |
| رمز التحديث: | 20240511 |
| مُعرف محوري في PubMed: | PMC11064546 |
| DOI: | 10.1128/spectrum.03784-23 |
| PMID: | 38511948 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2165-0497 |
|---|---|
| DOI: | 10.1128/spectrum.03784-23 |