دورية أكاديمية
Induction of SK-MEL-28 Invasion by Brain Cortical Cell-Conditioned Medium Through CXCL10 Signaling.
| العنوان: | Induction of SK-MEL-28 Invasion by Brain Cortical Cell-Conditioned Medium Through CXCL10 Signaling. |
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| المؤلفون: | Sampaio MCPD; Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, Brazil., Santos RVC; Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, Brazil., Albuquerque APB; Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, Brazil., Soares AKA; Fundação Altino Ventura, Recife, Brazil., Cordeiro MF; Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, Brazil., da Rosa MM; Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, Brazil., Pereira MC; Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, Brazil., da Rocha Pitta MG; Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, Brazil., Rêgo MJBM; Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, Brazil. |
| المصدر: | Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research [J Interferon Cytokine Res] 2024 May; Vol. 44 (5), pp. 198-207. Date of Electronic Publication: 2024 Mar 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 9507088 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7465 (Electronic) Linking ISSN: 10799907 NLM ISO Abbreviation: J Interferon Cytokine Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Larchmont, NY : Mary Ann Liebert Original Publication: New York, NY : Mary Ann Liebert, c1995- |
| مواضيع طبية MeSH: | Chemokine CXCL10*/metabolism , Chemokine CXCL10*/genetics , Signal Transduction* , Melanoma*/pathology , Melanoma*/metabolism , Cell Movement*, Humans ; Culture Media, Conditioned/pharmacology ; Cell Line, Tumor ; Interleukin-8/metabolism ; Brain Neoplasms/metabolism ; Brain Neoplasms/pathology ; Brain Neoplasms/secondary ; Neoplasm Invasiveness ; Cell Proliferation ; Cerebral Cortex/metabolism ; Cerebral Cortex/pathology |
| مستخلص: | Melanoma, an infrequent yet significant variant of skin cancer, emerges as a primary cause of brain metastasis among various malignancies. Despite recognizing the involvement of inflammatory molecules, particularly chemokines, in shaping the metastatic microenvironment, the intricate cellular signaling mechanisms underlying cerebral metastasis remain elusive. In our pursuit to unravel the role of cytokines in melanoma metastasis, we devised a protocol utilizing mixed cerebral cortical cells and SK-MEL-28 melanoma cell lines. Contrary to expectations, we observed no discernible morphological change in melanoma cells exposed to a cerebral conditioned medium (CM). However, a substantial increase in both migration and proliferation was quantitatively noted. Profiling the chemokine secretion by melanoma in response to the cerebral CM unveiled the pivotal role of interferon gamma-induced protein 10 (CXCL10), inhibiting the secretion of interleukin 8 (CXCL8). Furthermore, through a transwell assay, we demonstrated that knockdown CXCL10 led to a significant decrease in the migration of the SK-MEL-28 cell line. In conclusion, our findings suggest that a cerebral CM induces melanoma cell migration, while modulating the secretion of CXCL10 and CXCL8 in the context of brain metastases. These insights advance our understanding of the underlying mechanisms in melanoma cerebral metastasis, paving the way for further exploration and targeted therapeutic interventions. |
| فهرسة مساهمة: | Keywords: cerebral metastases; chemokines; skin cancer |
| المشرفين على المادة: | 0 (Chemokine CXCL10) 0 (Culture Media, Conditioned) 0 (CXCL10 protein, human) 0 (Interleukin-8) |
| تواريخ الأحداث: | Date Created: 20240321 Date Completed: 20240501 Latest Revision: 20240506 |
| رمز التحديث: | 20240506 |
| DOI: | 10.1089/jir.2023.0158 |
| PMID: | 38512222 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-7465 |
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| DOI: | 10.1089/jir.2023.0158 |