دورية أكاديمية
Identification of Peucedanum japonicum Thunb. extract components and their protective effects against dexamethasone-induced muscle atrophy.
| العنوان: | Identification of Peucedanum japonicum Thunb. extract components and their protective effects against dexamethasone-induced muscle atrophy. |
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| المؤلفون: | Kim YI; Aging and Metabolism Research Group, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, South Korea; Department of Food Science and Technology, Jeonbuk National University, Jeonju-si, South Korea., Lee H; Aging and Metabolism Research Group, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, South Korea., Kim MJ; Aging and Metabolism Research Group, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, South Korea., Jung CH; Aging and Metabolism Research Group, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, South Korea; Department of Food Biotechnology, Korea National University of Science and Technology, Daejeon, South Korea., Kim YS; Department of Food Science and Technology, Jeonbuk National University, Jeonju-si, South Korea., Ahn J; Aging and Metabolism Research Group, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, South Korea; Department of Food Biotechnology, Korea National University of Science and Technology, Daejeon, South Korea. Electronic address: jyan@kfri.re.kr. |
| المصدر: | Phytomedicine : international journal of phytotherapy and phytopharmacology [Phytomedicine] 2024 Jun; Vol. 128, pp. 155449. Date of Electronic Publication: 2024 Feb 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Urban & Fischer Verlag Country of Publication: Germany NLM ID: 9438794 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-095X (Electronic) Linking ISSN: 09447113 NLM ISO Abbreviation: Phytomedicine Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Stuttgart : Urban & Fischer Verlag Original Publication: Stuttgart ; New York : G. Fischer, c1994- |
| مواضيع طبية MeSH: | Muscular Atrophy*/chemically induced , Muscular Atrophy*/drug therapy , Dexamethasone*/pharmacology , Plant Extracts*/pharmacology , Plant Extracts*/chemistry , Mice, Inbred C57BL* , Apiaceae*/chemistry, Quinic Acid/*analogs & derivatives, Animals ; Mice ; Male ; Cell Line ; Tandem Mass Spectrometry ; Muscle Fibers, Skeletal/drug effects ; Quinic Acid/pharmacology ; Chromatography, High Pressure Liquid ; Myogenin/metabolism |
| مستخلص: | Background: Peucedanum japonicum Thunb. (PJ) is a vegetable widely consumed in East Asia and is known to have anticancer and anti-inflammatory effects. However, the effect of PJ on muscle atrophy remains elusive. Purpose: This study aimed to investigate the effect of PJ and its active compound on dexamethasone (DEX)-induced muscle atrophy. Methods: We performed qualitative and quantitative analysis of PJ using ultra-performance liquid chromatography-mass spectrometry tandem mass spectrometry (UPLC-MS/MS) and high-performance liquid chromatography (HPLC), respectively. The efficacy of PJ and its main compound 4-caffeoylquinic acid (CQA) on muscle atrophy was evaluated in DEX-induced myotube atrophy and DEX-induced muscle atrophy in mouse myoblasts (C2C12) and C57BL/6 mice, in vitro and in vivo, respectively. Results: The UPLC-MS/MS and HPLC data showed that the concentration of 4-CQA in PJ was 18.845 mg/g. PJ and 4-CQA treatments significantly inhibited DEX-induced myotube atrophy by decreasing protein synthesis and glucocorticoid translocation to the nucleus in C2C12 myotubes. In addition, PJ enhanced myogenesis by upregulating myogenin and myogenic differentiation 1 in C2C12 cells. PJ supplementation effectively increased muscle function and mass, downregulated atrogenes, and decreased proteasome activity in C57BL/6 mice. Additionally, PJ effectively decreased the nuclear translocation of forkhead transcription factor 3 alpha by inhibiting glucocorticoid receptor. Conclusion: Overall, PJ and its active compound 4-CQA alleviated skeletal muscle atrophy by inhibiting protein degradation. Hence, our findings present PJ as a potential novel pharmaceutical candidate for the treatment of muscle atrophy. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024. Published by Elsevier GmbH.) |
| فهرسة مساهمة: | Keywords: 4-caffeoylquinic acid; Dexamethasone; Muscle atrophy; Peucedanum japonicum Thunb; Protein degradation |
| المشرفين على المادة: | 7S5I7G3JQL (Dexamethasone) 0 (Plant Extracts) 0 (caffeoylquinic acid) 058C04BGYI (Quinic Acid) 0 (Myogenin) |
| تواريخ الأحداث: | Date Created: 20240322 Date Completed: 20240430 Latest Revision: 20240430 |
| رمز التحديث: | 20240430 |
| DOI: | 10.1016/j.phymed.2024.155449 |
| PMID: | 38518644 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1618-095X |
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| DOI: | 10.1016/j.phymed.2024.155449 |