دورية أكاديمية
Molecular insights into the catabolism of dibutyl phthalate in Pseudomonas aeruginosa PS1 based on biochemical and multi-omics approaches.
| العنوان: | Molecular insights into the catabolism of dibutyl phthalate in Pseudomonas aeruginosa PS1 based on biochemical and multi-omics approaches. |
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| المؤلفون: | Du H; Guangzhou Customs Technology Center, No. 66 Huacheng Avenue, Tianhe District, Guangzhou 510623, China; Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China; Center for Statistical Science, Tsinghua University, Beijing 100084, China., Cheng JL; Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China; Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, China., Li ZY; Guangzhou Customs Technology Center, No. 66 Huacheng Avenue, Tianhe District, Guangzhou 510623, China., Zhong HN; Guangzhou Customs Technology Center, No. 66 Huacheng Avenue, Tianhe District, Guangzhou 510623, China., Wei S; Guangzhou Customs Technology Center, No. 66 Huacheng Avenue, Tianhe District, Guangzhou 510623, China., Gu YJ; Guangzhou Customs Technology Center, No. 66 Huacheng Avenue, Tianhe District, Guangzhou 510623, China., Yao CC; Guangzhou Customs Technology Center, No. 66 Huacheng Avenue, Tianhe District, Guangzhou 510623, China., Zhang M; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, China., Cai QY; Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China., Zhao HM; Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: zhaohm99@jnu.edu.cn., Mo CH; Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. |
| المصدر: | The Science of the total environment [Sci Total Environ] 2024 May 20; Vol. 926, pp. 171852. Date of Electronic Publication: 2024 Mar 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Dibutyl Phthalate*/analysis , Pseudomonas aeruginosa*/genetics , Pseudomonas aeruginosa*/metabolism, Multiomics ; Tandem Mass Spectrometry ; Biodegradation, Environmental |
| مستخلص: | A comprehensive understanding of the molecular mechanisms underlying microbial catabolism of dibutyl phthalate (DBP) is still lacking. Here, we newly isolated a bacterial strain identified as Pseudomonas aeruginosa PS1 with high efficiency of DBP degradation. The degradation ratios of DBP at 100-1000 mg/L by this strain reached 80-99 % within 72 h without a lag phase. A rare DBP-degradation pathway containing two monobutyl phthalate-catabolism steps was proposed based on intermediates identified by HPLC-TOF-MS/MS. In combination with genomic and transcriptomic analyses, we identified 66 key genes involved in DBP biodegradation and revealed the genetic basis for a new complete catabolic pathway from DBP to Succinyl-CoA or Acetyl-CoA in the genus Pseudomonas for the first time. Notably, we found that a series of homologous genes in Pht and Pca clusters were simultaneously activated under DBP exposure and some key intermediate degradation related gene clusters including Pht, Pca, Xyl, Ben, and Cat exhibited a favorable coexisting pattern, which contributed the high-efficient DBP degradation ability and strong adaptability to this strain. Overall, these results broaden the knowledge of the catabolic diversity of DBP in microorganisms and enhance our understanding of the molecular mechanism underlying DBP biodegradation. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reports in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biodegradation; Dibutyl phthalate; Gene cluster; Genomics; Transcriptomics |
| المشرفين على المادة: | 2286E5R2KE (Dibutyl Phthalate) |
| تواريخ الأحداث: | Date Created: 20240322 Date Completed: 20240417 Latest Revision: 20240417 |
| رمز التحديث: | 20240417 |
| DOI: | 10.1016/j.scitotenv.2024.171852 |
| PMID: | 38518818 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1026 |
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| DOI: | 10.1016/j.scitotenv.2024.171852 |