دورية أكاديمية
أعرض في EDS

Electrochemical microfluidic immunosensor with graphene-decorated gold nanoporous for T-2 mycotoxin detection.

التفاصيل البيبلوغرافية
العنوان: Electrochemical microfluidic immunosensor with graphene-decorated gold nanoporous for T-2 mycotoxin detection.
المؤلفون: Fernandez Solis LN; Universidad Nacional de San Luis, Facultad de Química, Bioquímica y Farmacia, Instituto de Química de San Luis, INQUISAL (UNSL - CONICET), Chacabuco 917, D5700BWS, San Luis, Argentina., Silva Junior GJ; LSEME. Laboratório de Sensores Eletroquímicos e Métodos Eletroanalíticos. Institute of Chemistry, University of São Paulo, Av. Professor Lineu Prestes, 748, 05513-970, São Paulo - SP, Brazil., Bertotti M; LSEME. Laboratório de Sensores Eletroquímicos e Métodos Eletroanalíticos. Institute of Chemistry, University of São Paulo, Av. Professor Lineu Prestes, 748, 05513-970, São Paulo - SP, Brazil., Angnes L; Laboratório de Automação e Instrumentação Analítica, Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Professor Lineu Prestes 748, 05508-000, São Paulo, Brazil., Pereira SV; Universidad Nacional de San Luis, Facultad de Química, Bioquímica y Farmacia, Instituto de Química de San Luis, INQUISAL (UNSL - CONICET), Chacabuco 917, D5700BWS, San Luis, Argentina., Fernández-Baldo MA; Universidad Nacional de San Luis, Facultad de Química, Bioquímica y Farmacia, Instituto de Química de San Luis, INQUISAL (UNSL - CONICET), Chacabuco 917, D5700BWS, San Luis, Argentina. Electronic address: mbaldo@unsl.edu.ar., Regiart M; Universidad Nacional de San Luis, Facultad de Química, Bioquímica y Farmacia, Instituto de Química de San Luis, INQUISAL (UNSL - CONICET), Chacabuco 917, D5700BWS, San Luis, Argentina. Electronic address: mregiart@unsl.edu.ar.
المصدر: Talanta [Talanta] 2024 Jun 01; Vol. 273, pp. 125971. Date of Electronic Publication: 2024 Mar 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
مواضيع طبية MeSH: Graphite*/chemistry , Mycotoxins* , Nanopores* , T-2 Toxin* , Biosensing Techniques*/methods , Metal Nanoparticles*/chemistry, Immunoassay/methods ; Microfluidics ; Gold/chemistry ; Hydrogen Peroxide/chemistry ; Electrochemical Techniques/methods ; Limit of Detection
مستخلص: T-2 is one of the most potent cytotoxic food-borne mycotoxins. In this work, we have developed and characterized an electrochemical microfluidic immunosensor for T-2 toxin quantification in wheat germ samples. T-2 toxin detection was carried out using a competitive immunoassay method based on monoclonal anti-T-2 antibodies immobilized on the poly(methyl methacrylate) (PMMA) microfluidic central channel. The platinum wire working electrode at the end of the channel was in situ modified by a single-step electrodeposition procedure with reduced graphene oxide (rGO)-nanoporous gold (NPG). T-2 toxin in the sample was allowed to compete with T-2-horseradish peroxidase (HRP) conjugated for the specific recognizing sites of immobilized anti-T-2 monoclonal antibodies. The HRP, in the presence of hydrogen peroxide (H 2 O 2 ), catalyzes the oxidation of 4-tert-butylcatechol (4-TBC), whose back electrochemical reduction was detected on the nanostructured electrode at -0.15 V. Thus, at low T-2 concentrations in the sample, more enzymatically conjugated T-2 will bind to the capture antibodies, and, therefore, a higher current is expected. The detection limits found for electrochemical immunosensor, and commercial ELISA procedure were 0.10 μg kg -1 and 10 μg kg -1 , and the intra- and inter-assay coefficients of variation were below 5.35% and 6.87%, respectively. Finally, our microfluidic immunosensor to T-2 toxin will significantly contribute to faster, direct, and secure in situ analysis in agricultural samples.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Electrochemical; Microfluidic immunosensor; Mycotoxins; Nanomaterial; T-2 toxin
المشرفين على المادة: 7782-42-5 (Graphite)
0 (Mycotoxins)
7440-57-5 (Gold)
I3FL5NM3MO (T-2 Toxin)
BBX060AN9V (Hydrogen Peroxide)
تواريخ الأحداث: Date Created: 20240323 Date Completed: 20240409 Latest Revision: 20240409
رمز التحديث: 20240409
DOI: 10.1016/j.talanta.2024.125971
PMID: 38521020
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-3573
DOI:10.1016/j.talanta.2024.125971