دورية أكاديمية
Histological changes in the olfactory bulb and rostral migratory stream due to interruption of olfactory input.
| العنوان: | Histological changes in the olfactory bulb and rostral migratory stream due to interruption of olfactory input. |
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| المؤلفون: | Nakamura Y; Department of Otorhinolaryngology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku, Japan., Miwa T; Department of Otorhinolaryngology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku, Japan. Electronic address: miwataka@kanazawa-med.ac.jp., Shiga H; Department of Otorhinolaryngology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku, Japan., Sakata H; Department of Anatomy I, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku, Japan., Shigeta D; Department of Anatomy I, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku, Japan., Hatta T; Department of Anatomy I, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku, Japan. |
| المصدر: | Auris, nasus, larynx [Auris Nasus Larynx] 2024 Jun; Vol. 51 (3), pp. 517-524. Date of Electronic Publication: 2024 Mar 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 7708170 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1476 (Electronic) Linking ISSN: 03858146 NLM ISO Abbreviation: Auris Nasus Larynx Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science Original Publication: Tokyo, Society for Promotion of International Otorhinolaryngology. |
| مواضيع طبية MeSH: | Olfactory Bulb*/pathology , Olfactory Bulb*/drug effects , Olfactory Bulb*/cytology , Methimazole*/pharmacology , Cell Movement*, Animals ; Mice ; Antithyroid Agents/pharmacology ; Olfactory Nerve/pathology ; Olfactory Marker Protein/metabolism ; Disease Models, Animal ; Male |
| مستخلص: | Objective: Periglomerular and granule cells in the adult mammalian olfactory bulb modulate olfactory signal transmission. These cells originate from the subventricular zone, migrate to the olfactory bulb via the Rostral Migratory Stream (RMS), and differentiate into mature cells within the olfactory bulb throughout postnatal life. While the regulation of neuroblast development is known to be affected by external stimuli, there is a lack of information concerning changes that occur during the recovery process after injury caused by external stimuli. To address this gap in research, the present study conducted histological observations to investigate changes in the olfactory bulb and RMS occurring after the degeneration and regeneration of olfactory neurons. Methods: To create a model of olfactory neurodegeneration, adult mice were administered methimazole intraperitoneally. Nasal tissue and whole brains were removed 3, 7, 14 and 28 days after methimazole administration, and EdU was administered 2 and 4 h before removal of these tissues to monitor dividing cells in the RMS. Methimazole-untreated mice were used as controls. Olfactory nerve fibers entering the olfactory glomerulus were observed immunohistochemically using anti-olfactory marker protein. In the brain tissue, the entire RMS was observed and the volume and total number of cells in the RMS were measured. In addition, the number of neuroblasts and dividing neuroblasts passing through the RMS were measured using anti-doublecortin and anti-EdU antibodies, respectively. Statistical analysis was performed using the Tukey test. Results: Olfactory epithelium degenerated was observed after methimazole administration, and recovered after 28 days. In the olfactory glomeruli, degeneration of OMP fibers began after methimazole administration, and after day 14, OMP fibers were reduced or absent by day 28, and overall OMP positive fibers were less than 20%. Glomerular volume tended to decrease after methimazole administration and did not appear to recover, even 28 days after recovery of the olfactory epithelium. In the RMS, EdU-positive cells decreased on day 3 and began to increase on day 7. However, they did not recover to the same levels as the control methimazole-untreated mice even after 28 days. Conclusion: These results suggest that the division and maturation of neuroblasts migrating from the RMS was suppressed by olfactory nerve degeneration or the disruption of olfactory input. Competing Interests: Declaraion of competing interest The authors declare no conflicts of interest associated with this manuscript. (Copyright © 2024. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Neuroblast; Neurogenesis; Olfactory bulb; Olfactory disorder; Olfactory epithelium; Rostral migratory stream |
| المشرفين على المادة: | 554Z48XN5E (Methimazole) 0 (Antithyroid Agents) 0 (Olfactory Marker Protein) |
| تواريخ الأحداث: | Date Created: 20240324 Date Completed: 20240509 Latest Revision: 20240509 |
| رمز التحديث: | 20240510 |
| DOI: | 10.1016/j.anl.2024.01.009 |
| PMID: | 38522356 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1879-1476 |
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| DOI: | 10.1016/j.anl.2024.01.009 |