دورية أكاديمية
A novel bHLH gene responsive to low nitrogen positively regulates the biosynthesis of medicinal tropane alkaloids in Atropa belladonna.
| العنوان: | A novel bHLH gene responsive to low nitrogen positively regulates the biosynthesis of medicinal tropane alkaloids in Atropa belladonna. |
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| المؤلفون: | Gou Y; Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City State Key Laboratory of Silkworm Genome Biology, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing 400715, China., Jing Y; Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City State Key Laboratory of Silkworm Genome Biology, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing 400715, China., Song J; Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City State Key Laboratory of Silkworm Genome Biology, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing 400715, China., Nagdy MM; College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China; Department of Medicinal and Aromatic Plants Research, National Research Centre, 12311 Dokki, Cairo, Egypt., Peng C; Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City State Key Laboratory of Silkworm Genome Biology, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing 400715, China., Zeng L; Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City State Key Laboratory of Silkworm Genome Biology, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing 400715, China., Chen M; College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China., Lan X; TAAHC-SWU Medicinal Plant Joint R&D Centre, The Provincial and Ministerial Co-founded Collaborative Innovation Center for R&D in Xizang Characteristic Agricultural and Animal Husbandry Resources, Tibet Agriculture and Animal Husbandry College, Nyingchi of Xizang 860000, China., Htun ZLL; Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City State Key Laboratory of Silkworm Genome Biology, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing 400715, China; Department of Botany, University of Magway, Magway 04012, Myanmar., Liao Z; Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City State Key Laboratory of Silkworm Genome Biology, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing 400715, China. Electronic address: zhliao@swu.edu.cn., Li Y; Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City State Key Laboratory of Silkworm Genome Biology, SWU-TAAHC Medicinal Plant Joint R&D Centre, School of Life Sciences, Southwest University, Chongqing 400715, China. Electronic address: liyan20230111@swu.edu.cn. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 May; Vol. 266 (Pt 1), pp. 131012. Date of Electronic Publication: 2024 Mar 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Nitrogen*/metabolism , Gene Expression Regulation, Plant*/drug effects , Atropa belladonna*/metabolism , Atropa belladonna*/genetics , Tropanes*/metabolism , Basic Helix-Loop-Helix Transcription Factors*/genetics , Basic Helix-Loop-Helix Transcription Factors*/metabolism , Mixed Function Oxygenases*, Plant Proteins/genetics ; Plant Proteins/metabolism ; Plants, Medicinal/metabolism ; Plants, Medicinal/genetics ; Hyoscyamine/metabolism ; Hyoscyamine/genetics ; Scopolamine/metabolism ; Promoter Regions, Genetic |
| مستخلص: | Medicinal tropane alkaloids (TAs), including hyoscyamine, anisodamine and scopolamine, are essential anticholinergic drugs specifically produced in several solanaceous plants. Atropa belladonna is one of the most important medicinal plants that produces TAs. Therefore, it is necessary to cultivate new A. belladonna germplasm with the high content of TAs. Here, we found that the levels of TAs were elevated under low nitrogen (LN) condition, and identified a LN-responsive bHLH transcription factor (TF) of A. belladonna (named LNIR) regulating the biosynthesis of TAs. The expression level of LNIR was highest in secondary roots where TAs are synthesized specifically, and was significantly induced by LN. Further research revealed that LNIR directly activated the transcription of hyoscyamine 6β-hydroxylase gene (H6H) by binding to its promoter, which converts hyoscyamine into anisodamine and subsequently epoxidizes anisodamine to form scopolamine. Overexpression of LNIR upregulated the expression levels of TA biosynthesis genes and consequently led to the increased production of TAs. In summary, we functionally identified a LN-responsive bHLH gene that facilitated the development of A. belladonna with high-yield TAs under the decreased usage of nitrogen fertilizer. Competing Interests: Declaration of competing interest The authors declare that they have no competing interests. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Atropa belladonna; Biosynthesis; Low nitrogen; Tropane alkaloids; bHLH gene |
| المشرفين على المادة: | N762921K75 (Nitrogen) 0 (Tropanes) 0 (Basic Helix-Loop-Helix Transcription Factors) 0 (Plant Proteins) PX44XO846X (Hyoscyamine) DL48G20X8X (Scopolamine) EC 1.14.11.11 (hyoscyamine (6S)-dioxygenase) EC 1.- (Mixed Function Oxygenases) |
| تواريخ الأحداث: | Date Created: 20240324 Date Completed: 20240430 Latest Revision: 20240430 |
| رمز التحديث: | 20240501 |
| DOI: | 10.1016/j.ijbiomac.2024.131012 |
| PMID: | 38522709 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2024.131012 |