دورية أكاديمية
Stress-induced microautophagy is coordinated with lysosome biogenesis and regulated by PIKfyve.
| العنوان: | Stress-induced microautophagy is coordinated with lysosome biogenesis and regulated by PIKfyve. |
|---|---|
| المؤلفون: | Klein AD; BCMB Graduate Program, Weill Cornell Medical College, New York, NY 10065.; Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065., Petruzzi KL; Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065., Lee C; BCMB Graduate Program, Weill Cornell Medical College, New York, NY 10065.; Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065., Overholtzer M; BCMB Graduate Program, Weill Cornell Medical College, New York, NY 10065.; Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065.; Gerstner Sloan Kettering Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY 10065. |
| المصدر: | Molecular biology of the cell [Mol Biol Cell] 2024 May 01; Vol. 35 (5), pp. ar70. Date of Electronic Publication: 2024 Mar 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Cell Biology Country of Publication: United States NLM ID: 9201390 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1939-4586 (Electronic) Linking ISSN: 10591524 NLM ISO Abbreviation: Mol Biol Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD : American Society for Cell Biology, c1992- |
| مواضيع طبية MeSH: | Lysosomes*/metabolism , Microautophagy*, Autophagy ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ; Intracellular Membranes/metabolism ; Ionophores ; Humans ; Cell Line, Tumor |
| مستخلص: | Lysosome turnover and biogenesis are induced in response to treatment of cells with agents that cause membrane rupture, but whether other stress conditions engage similar homeostatic mechanisms is not well understood. Recently we described a form of selective turnover of lysosomes that is induced by metabolic stress or by treatment of cells with ionophores or lysosomotropic agents, involving the formation of intraluminal vesicles within intact organelles through microautophagy. Selective turnover involves noncanonical autophagy and the lipidation of LC3 onto lysosomal membranes, as well as the autophagy gene-dependent formation of intraluminal vesicles. Here, we find a form of microautophagy induction that requires activity of the lipid kinase PIKfyve and is associated with the nuclear translocation of TFEB, a known mediator of lysosome biogenesis. We show that LC3 undergoes turnover during this process, and that PIKfyve is required for the formation of intraluminal vesicles and LC3 turnover, but not for LC3 lipidation onto lysosomal membranes, demonstrating that microautophagy is regulated by PIKfyve downstream of noncanonical autophagy. We further show that TFEB activation requires noncanonical autophagy but not PIKfyve, distinguishing the regulation of biogenesis from microautophagy occurring in response to agents that induce lysosomal stress. |
| References: | Science. 2018 Apr 6;360(6384):. (PMID: 29622626) Dev Cell. 2016 Sep 12;38(5):536-47. (PMID: 27623384) Nature. 2007 Dec 20;450(7173):1253-7. (PMID: 18097414) Proc Natl Acad Sci U S A. 2020 Aug 25;117(34):20803-20813. (PMID: 32764148) FEBS Lett. 2016 Jun;590(11):1576-85. (PMID: 27135648) Dev Cell. 2013 Sep 16;26(5):511-24. (PMID: 23993788) Nat Cell Biol. 2020 Feb;22(2):187-199. (PMID: 31932738) EMBO J. 2018 Nov 2;37(21):. (PMID: 30314966) EMBO J. 1998 Jul 1;17(13):3597-607. (PMID: 9649430) J Cell Sci. 2018 May 21;131(10):. (PMID: 29661845) EMBO Rep. 2023 Dec 6;24(12):e57300. (PMID: 37987447) Nat Cell Biol. 2020 Oct;22(10):1252-1263. (PMID: 32989250) Dev Cell. 2020 Nov 9;55(3):289-297.e4. (PMID: 32916093) Sci Adv. 2022 Oct 28;8(43):eabo1274. (PMID: 36288315) Nat Cell Biol. 2011 Oct 16;13(11):1335-43. (PMID: 22002674) Cells. 2020 Sep 01;9(9):. (PMID: 32882854) Sci Adv. 2021 Oct;7(40):eabj2485. (PMID: 34597140) Trends Cell Biol. 2012 Jul;22(7):374-80. (PMID: 22608991) Chem Biol. 2013 Jul 25;20(7):912-21. (PMID: 23890009) Science. 2009 Jul 24;325(5939):473-7. (PMID: 19556463) Cell. 2001 Jul 27;106(2):145-55. (PMID: 11511343) Dev Cell. 2002 Aug;3(2):271-82. (PMID: 12194857) J Cell Biol. 2008 Jan 28;180(2):389-402. (PMID: 18209100) Autophagy. 2015;11(1):88-99. (PMID: 25484071) Nature. 2000 Nov 23;408(6811):488-92. (PMID: 11100732) J Biol Chem. 2017 May 19;292(20):8424-8435. (PMID: 28360104) Nat Commun. 2012 Mar 13;3:731. (PMID: 22415822) Autophagy. 2017 May 4;13(5):854-867. (PMID: 28296541) EMBO J. 2013 Aug 28;32(17):2336-47. (PMID: 23921551) J Cell Biol. 2018 Aug 6;217(8):2765-2776. (PMID: 29848618) Mol Biol Cell. 2022 Mar 1;33(3):ar26. (PMID: 35020443) Biochem Biophys Res Commun. 2022 Jul 23;614:161-168. (PMID: 35597153) Nat Commun. 2010 Jul 13;1:38. (PMID: 20802798) EMBO Rep. 2008 Feb;9(2):164-70. (PMID: 18188180) Elife. 2017 Jun 07;6:. (PMID: 28590904) |
| معلومات مُعتمدة: | P30 CA008748 United States CA NCI NIH HHS; R35 CA263846 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors) 0 (Ionophores) EC 2.7.1.137 (PIKFYVE protein, human) |
| تواريخ الأحداث: | Date Created: 20240327 Date Completed: 20240418 Latest Revision: 20240702 |
| رمز التحديث: | 20240702 |
| مُعرف محوري في PubMed: | PMC11151102 |
| DOI: | 10.1091/mbc.E23-08-0332 |
| PMID: | 38536415 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1939-4586 |
|---|---|
| DOI: | 10.1091/mbc.E23-08-0332 |