دورية أكاديمية
Polyphenol analysis in human milk by a rapid, cost-effective, and accurate micromethod: translational development.
العنوان: | Polyphenol analysis in human milk by a rapid, cost-effective, and accurate micromethod: translational development. |
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المؤلفون: | Cortez MV; Universidad Nacional de Córdoba, Facultad de Ciencias Médicas, Escuela de Fonoaudiología, Ciudad de Córdoba, Córdoba, Argentina., Scotta AV; Universidad Nacional de Córdoba, Facultad de Ciencias Médicas, Escuela de Fonoaudiología, Ciudad de Córdoba, Córdoba, Argentina., Miranda AR; Montpellier Interdisciplinary Center on Sustainable Agri-food Systems (MoISA), University of Montpellier, CIRAD, CIHEAM-IAMM, INRAE, Institut Agro, IRD, Montpellier, France., Soria EA; Universidad Nacional de Córdoba, Facultad de Ciencias Médicas, Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Ciudad de Córdoba, Córdoba, Argentina. |
المصدر: | Revista paulista de pediatria : orgao oficial da Sociedade de Pediatria de Sao Paulo [Rev Paul Pediatr] 2024 Mar 25; Vol. 42, pp. e2023186. Date of Electronic Publication: 2024 Mar 25 (Print Publication: 2024). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Sociedade De Pediatria De Sao Paulo Country of Publication: Brazil NLM ID: 9109353 Publication Model: eCollection Cited Medium: Internet ISSN: 1984-0462 (Electronic) Linking ISSN: 01030582 NLM ISO Abbreviation: Rev Paul Pediatr Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2017- : Sao Paulo : Sociedade De Pediatria De Sao Paulo Original Publication: Sao Paulo : Sociedade De Pediatria De Sao Paulo |
مواضيع طبية MeSH: | Polyphenols*/analysis , Milk, Human*/chemistry , Diazonium Compounds*, Infant, Newborn ; Humans ; Bicarbonates ; Cost-Benefit Analysis |
مستخلص: | Objective: To develop a rapid method for analysing polyphenols, which are potentially active antioxidants against neonatal oxidative stress, from small human milk (HM) volumes. Methods: Acid and alkaline extractions were compared using two dyes: Folin-Ciocalteu and Fast Blue BB. Linearity, sensitivity, recovery percentage, polyphenol content, precision, and stability were assessed in 14 HM samples and compared using the Kruskal-Wallis H test (p<0.05). The best technique was applied to 284 HM samples to determine their polyphenolic content and its association with maternal diet by multifactorial linear regression. Results: Acidic extraction successfully recovered the gallic acid reference standard, whereas alkaline extraction overestimated it. Calibration curves for all methods were linear (R2>0.96) up to 500 mg/L. All bicarbonate-based Folin-Ciocalteu methods assayed were stable and repeatable, whereas Fast Blue BB-based variants were not. HM polyphenols (mean=94.68 mg/L) positively correlated to the dietary intake of hydroxycinnamic acids, the most consumed polyphenolic family in this population. Conclusions: A bicarbonate-based Folin-Ciocalteu micromethod allowed the accurate determination of polyphenols in HM, which might be useful for translational research settings and HM banks. |
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المشرفين على المادة: | 0 (Polyphenols) 15518-68-0 (Fast Blue BB) 0 (Bicarbonates) 0 (Diazonium Compounds) |
تواريخ الأحداث: | Date Created: 20240327 Date Completed: 20240329 Latest Revision: 20240329 |
رمز التحديث: | 20240329 |
مُعرف محوري في PubMed: | PMC10962630 |
DOI: | 10.1590/1984-0462/2024/42/2023186 |
PMID: | 38537035 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1984-0462 |
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DOI: | 10.1590/1984-0462/2024/42/2023186 |