دورية أكاديمية
The ex vivo perfused mouse adrenal gland-a new model to study aldosterone secretion.
| العنوان: | The ex vivo perfused mouse adrenal gland-a new model to study aldosterone secretion. |
|---|---|
| المؤلفون: | Plain A; Medical Cell Biology, University of Regensburg, Regensburg, Germany., Knödl L; Medical Cell Biology, University of Regensburg, Regensburg, Germany., Tegtmeier I; Medical Cell Biology, University of Regensburg, Regensburg, Germany., Bandulik S; Medical Cell Biology, University of Regensburg, Regensburg, Germany., Warth R; Medical Cell Biology, University of Regensburg, Regensburg, Germany. richard.warth@ur.de. |
| المصدر: | Pflugers Archiv : European journal of physiology [Pflugers Arch] 2024 Jun; Vol. 476 (6), pp. 911-922. Date of Electronic Publication: 2024 Mar 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 0154720 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2013 (Electronic) Linking ISSN: 00316768 NLM ISO Abbreviation: Pflugers Arch Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, New York, Springer. |
| مواضيع طبية MeSH: | Aldosterone*/metabolism , Adrenal Glands*/metabolism, Animals ; Mice ; Mice, Inbred C57BL ; Male ; Perfusion/methods ; Zona Glomerulosa/metabolism |
| مستخلص: | Aldosterone is a steroid hormone that is important for maintaining the volume and ionic composition of extracellular fluids and is produced in the zona glomerulosa of the adrenal cortex. The basic mechanisms controlling aldosterone secretion are known. However, more detailed studies on the regulation of aldosterone secretion often fail due to the lack of suitable models: although secretion can be studied in cultured adrenocortical cells under defined conditions, the differentiation status of the cells is difficult to control and the complex anatomy of the adrenal cortex is lost. In living animals, the physiological context is intact, but the influences are manifold and the examination conditions cannot be sufficiently controlled. One method that closes the gap between cell models and studies in living animals is the isolated perfused adrenal gland. In the past, this method has provided important data on the pathophysiology of adrenal glands from larger animals, but the technique was not used in mice. Here, we developed a method for isolation and perfusion of the mouse adrenal gland to study aldosterone secretion. This technique preserves the complex anatomical and functional context of the mouse adrenal cortex, to ensure defined experimental conditions and to minimize extra-adrenal influences. Initial series of experiments with the ex vivo perfused mouse adrenal gland show that this model offers the possibility for unique insights into pathophysiological regulatory principles and is suitable for the use of genetically modified mouse models. (© 2024. The Author(s).) |
| References: | Horm Metab Res. 2010 Jun;42(6):450-7. (PMID: 20049674) Hypertension. 2022 May;79(5):887-897. (PMID: 35139664) Horm Metab Res. 2010 Jun;42(6):446-9. (PMID: 20013646) Nat Rev Endocrinol. 2020 Oct;16(10):578-589. (PMID: 32724183) Biochem Biophys Res Commun. 1986 Aug 29;139(1):46-51. (PMID: 3767963) Int J Mol Sci. 2022 Nov 19;23(22):. (PMID: 36430866) Annu Rev Physiol. 2021 Feb 10;83:451-475. (PMID: 33176563) Endocrine. 2004 Jun;24(1):61-71. (PMID: 15249705) Hypertension. 2012 May;59(5):999-1005. (PMID: 22493079) Nat Genet. 2018 Mar;50(3):355-361. (PMID: 29403012) Endocrinology. 2016 Jun;157(6):2489-99. (PMID: 27035656) Am J Physiol. 1989 Jun;256(6 Pt 1):E760-4. (PMID: 2735401) Am J Physiol. 1997 Jan;272(1 Pt 2):R183-7. (PMID: 9039007) Clin Sci (Lond). 2018 Jan 16;132(1):145-156. (PMID: 29222092) Pflugers Arch. 2015 May;467(5):1027-42. (PMID: 25339223) J Endocrinol. 1986 Dec;111(3):391-6. (PMID: 3805967) J Mol Endocrinol. 2009 May;42(5):407-13. (PMID: 19221003) Endocrinology. 1985 Apr;116(4):1621-6. (PMID: 3971930) Methods Mol Biol. 2023;2565:91-103. (PMID: 36205889) J Vis Exp. 2016 Nov 17;(117):. (PMID: 27911373) J Physiol. 1961 Nov;159:40-57. (PMID: 13887557) Endocrinology. 1984 Dec;115(6):2195-204. (PMID: 6499766) Endocrinology. 2013 Aug;154(8):2712-22. (PMID: 23698720) J Physiol. 1970 Aug;209(3):539-56. (PMID: 4322584) Trends Cardiovasc Med. 2022 May;32(4):228-233. (PMID: 33775861) Endocrinology. 2012 Oct;153(10):4740-8. (PMID: 22878402) Am J Physiol. 1987 Jan;252(1 Pt 1):E118-23. (PMID: 3028148) J Endocrinol. 1981 Nov;91(2):313-23. (PMID: 6271893) Ann Anat. 2017 Sep;213:69-77. (PMID: 28578926) Nat Genet. 2013 Apr;45(4):440-4, 444e1-2. (PMID: 23416519) Physiol Rev. 2004 Apr;84(2):489-539. (PMID: 15044681) Endocrinology. 2014 Apr;155(4):1353-62. (PMID: 24506072) Am J Physiol. 1988 Mar;254(3 Pt 1):E243-7. (PMID: 3348386) Ann Anat. 2021 May;235:151679. (PMID: 33516785) |
| معلومات مُعتمدة: | WA 1275/6 and 471535567 Deutsche Forschungsgemeinschaft |
| فهرسة مساهمة: | Keywords: Adrenal cortex; Aldosteronism; Mouse models; Pathophysiology; Secretion |
| المشرفين على المادة: | 4964P6T9RB (Aldosterone) |
| تواريخ الأحداث: | Date Created: 20240328 Date Completed: 20240530 Latest Revision: 20240603 |
| رمز التحديث: | 20240603 |
| مُعرف محوري في PubMed: | PMC11139715 |
| DOI: | 10.1007/s00424-024-02950-z |
| PMID: | 38538989 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1432-2013 |
|---|---|
| DOI: | 10.1007/s00424-024-02950-z |