دورية أكاديمية
أعرض في EDS

Changes in Gravitaxis and Gene-Expression in an Euglena gracilis Culture over Time.

التفاصيل البيبلوغرافية
العنوان: Changes in Gravitaxis and Gene-Expression in an Euglena gracilis Culture over Time.
المؤلفون: Krüger J; Gravitational Biology Group, Department of Biology, Friedrich-Alexander University, Staudtstraße 5, 91058 Erlangen, Germany., Richter P; Gravitational Biology Group, Department of Biology, Friedrich-Alexander University, Staudtstraße 5, 91058 Erlangen, Germany., Stoltze J; Gravitational Biology Group, Department of Biology, Friedrich-Alexander University, Staudtstraße 5, 91058 Erlangen, Germany., Prasad B; Gravitational Biology Group, Department of Biology, Friedrich-Alexander University, Staudtstraße 5, 91058 Erlangen, Germany., Strauch SM; Gravitational Biology Group, Department of Biology, Friedrich-Alexander University, Staudtstraße 5, 91058 Erlangen, Germany.; Postgraduate Program in Health and Environment, University of Joinville Region, Rua Paulo Malschitzki, 10-Zona Industrial Norte, Joinville 89219-710, SC, Brazil., Krüger M; Environmental Cell Biology Group, Department of Microgravity and Translational Regenerative Medicine, Otto von Guericke University, 39106 Magdeburg, Germany.; Research Group 'Magdeburger Arbeitsgemeinschaft für Forschung unter Raumfahrt- und Schwerelosigkeitsbedingungen' (MARS), Otto von Guericke University, 39106 Magdeburg, Germany., Nasir A; Univ Brest, INSERM, EFS, UMR 1078, GGB-GTCA, 29200 Brest, France., Lebert M; Gravitational Biology Group, Department of Biology, Friedrich-Alexander University, Staudtstraße 5, 91058 Erlangen, Germany.
المصدر: Biomolecules [Biomolecules] 2024 Mar 09; Vol. 14 (3). Date of Electronic Publication: 2024 Mar 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101596414 Publication Model: Electronic Cited Medium: Internet ISSN: 2218-273X (Electronic) Linking ISSN: 2218273X NLM ISO Abbreviation: Biomolecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, 2011-
مواضيع طبية MeSH: Euglena gracilis*/genetics, Reproducibility of Results ; Phototaxis ; Photosynthesis ; Signal Transduction
مستخلص: Age-dependent changes in the transcription levels of 5-day-old Euglena gracilis cells, which showed positive gravitaxis, 6-day-old cells without gravitactic orientation, and older cells (9- and 11-day-old, which displayed a precise negative gravitaxis) were determined through microarray analysis. Hierarchical clustering of four independent cell cultures revealed pronounced similarities in transcription levels at the same culture age, which proves the reproducibility of the cultivation method. Employing the non-oriented cells from the 6-day-old culture as a reference, about 2779 transcripts were found to be differentially expressed. While positively gravitactic cells (5-day-old culture) showed only minor differences in gene expression compared to the 6-day reference, pronounced changes of mRNAs (mainly an increase) were found in older cells compared to the reference culture. Among others, genes coding for adenylyl cyclases, photosynthesis, and metabolic enzymes were identified to be differentially expressed. The investigated cells were grown in batch cultures, so variations in transcription levels most likely account for factors such as nutrient depletion in the medium and self-shading. Based on these findings, a particular transcript (e.g., transcript 19556) was downregulated using the RNA interference technique. Gravitaxis and phototaxis were impaired in the transformants, indicating the role of this transcript in signal transduction. Results of the experiment are discussed regarding the increasing importance of E. gracilis in biotechnology as a source of valuable products and the possible application of E. gracilis in life-support systems.
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معلومات مُعتمدة: 50WB1128 and 50WB1528 German Aerospace Center
فهرسة مساهمة: Keywords: Euglena gracilis; culture age; gravitaxis; microarray analysis; phototaxis
تواريخ الأحداث: Date Created: 20240328 Date Completed: 20240329 Latest Revision: 20240330
رمز التحديث: 20240330
مُعرف محوري في PubMed: PMC10967724
DOI: 10.3390/biom14030327
PMID: 38540747
قاعدة البيانات: MEDLINE
الوصف
تدمد:2218-273X
DOI:10.3390/biom14030327