دورية أكاديمية
أعرض في EDS

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water.

التفاصيل البيبلوغرافية
العنوان: Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water.
المؤلفون: Lopes RN; Electrical Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-598, Brazil.; Programa de Pós-Graduação em Biotecnologia Vegetal e Bioprocessos, CCS, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-598, Brazil., Pinto PHS; Electrical Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-598, Brazil., Vargas JDL; Nanotechnology Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-598, Brazil., Dante A; Electrical Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-598, Brazil., Macrae A; Programa de Pós-Graduação em Biotecnologia Vegetal e Bioprocessos, CCS, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-598, Brazil., Allil RCB; Electrical Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-598, Brazil., Werneck MM; Electrical Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-598, Brazil.; Nanotechnology Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-598, Brazil.
المصدر: Polymers [Polymers (Basel)] 2024 Mar 21; Vol. 16 (6). Date of Electronic Publication: 2024 Mar 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101545357 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4360 (Electronic) Linking ISSN: 20734360 NLM ISO Abbreviation: Polymers (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مستخلص: Conventional methods for pathogen detection in water rely on time-consuming enrichment steps followed by biochemical identification strategies, which require assay times ranging from 24 hours to a week. However, in recent years, significant efforts have been made to develop biosensing technologies enabling rapid and close-to-real-time detection of waterborne pathogens. In previous studies, we developed a plastic optical fiber (POF) immunosensor using an optoelectronic configuration consisting of a U-Shape probe connected to an LED and a photodetector. Bacterial detection was evaluated with the immunosensor immersed in a bacterial suspension in water with a known concentration. Here, we report on the sensitivity of a new optoelectronic configuration consisting of two POF U-shaped probes, one as the reference and the other as the immunosensor, for the detection of Escherichia coli . In addition, another methos of detection was tested where the sensors were calibrated in the air, before being immersed in a bacterial suspension and then read in the air. This modification improved sensor sensitivity and resulted in a faster detection time. After the immunocapture, the sensors were DAPI-stained and submitted to confocal microscopy. The histograms obtained confirmed that the responses of the immunosensors were due to the bacteria. This new sensor detected the presence of E. coli at 10 4 CFU/mL in less than 20 min. Currently, sub-20 min is faster than previous studies using fiber-optic based biosensors. We report on an inexpensive and faster detection technology when compared with conventional methods.
References: Biosensors (Basel). 2022 Aug 12;12(8):. (PMID: 36005031)
Biosensors (Basel). 2022 Oct 21;12(10):. (PMID: 36291044)
Biosens Bioelectron. 2011 Dec 15;30(1):328-32. (PMID: 21993139)
Anal Bioanal Chem. 2012 Jan;402(1):117-27. (PMID: 21956262)
Biosensors (Basel). 2022 Jul 28;12(8):. (PMID: 36004971)
Electrophoresis. 2016 Feb;37(3):529-35. (PMID: 26534833)
Sensors (Basel). 2018 Feb 22;18(2):. (PMID: 29470426)
Analyst. 2018 Jan 15;143(2):359-373. (PMID: 29271425)
Sensors (Basel). 2021 Mar 01;21(5):. (PMID: 33804378)
Sensors (Basel). 2017 Dec 19;17(12):. (PMID: 29257045)
Biosens Bioelectron. 2014 Apr 15;54:661-6. (PMID: 24334281)
Anal Chem. 2020 Jan 7;92(1):397-430. (PMID: 31665884)
Anal Bioanal Chem. 2002 Mar;372(5-6):664-82. (PMID: 11941437)
Appl Opt. 2021 Sep 10;60(26):8137-8144. (PMID: 34613077)
Sensors (Basel). 2022 Jan 19;22(3):. (PMID: 35161497)
Microbiologyopen. 2016 Dec;5(6):901-922. (PMID: 27397728)
Int J Microbiol. 2020 Jun 15;2020:2534130. (PMID: 32612658)
معلومات مُعتمدة: Finance Code 001 Coordenação de Aperfeicoamento de Pessoal de Nível Superior
فهرسة مساهمة: Keywords: Escherichia coli; biosensor; immunosensor; plastic optical fiber sensor
تواريخ الأحداث: Date Created: 20240328 Latest Revision: 20240330
رمز التحديث: 20240330
مُعرف محوري في PubMed: PMC10975820
DOI: 10.3390/polym16060861
PMID: 38543466
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4360
DOI:10.3390/polym16060861