دورية أكاديمية
Molecular Determination of Tumor Necrosis Factor-alpha, Interleukin-8, Interleukin-10, and C-X-C Chemokine Receptor-2 Genetic Variations and their Association with Disease Susceptibility and Mortality in COVID-19 Patients.
| العنوان: | Molecular Determination of Tumor Necrosis Factor-alpha, Interleukin-8, Interleukin-10, and C-X-C Chemokine Receptor-2 Genetic Variations and their Association with Disease Susceptibility and Mortality in COVID-19 Patients. |
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| المؤلفون: | Alsayed BA; Department of Internal Medicine, Faculty of Medicine, University of Tabuk, Tabuk, 71491, Saudi Arabia., Mir R; Department of Medical Lab technology Prince Fahad Bin Sultan Chair for Biomedical Research, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, 71491, Saudi Arabia., Mir MM; Department of Basic Medical Sciences (Biochemistry), College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia., Alnour TMS; Department of Medical Lab technology Prince Fahad Bin Sultan Chair for Biomedical Research, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, 71491, Saudi Arabia., Fawzy S; Department of Medical Microbiology, Faculty of Medicine, University of Tabuk, Tabuk, 71491, Saudi Arabia., M Ahmed M; Department of Medical Microbiology, Faculty of Medicine, University of Tabuk, Tabuk, 71491, Saudi Arabia., Amle D; Department of Biochemistry, All India Institute of Medical Sciences, Nagpur, 441108, India. |
| المصدر: | Current genomics [Curr Genomics] 2024 Feb 23; Vol. 25 (1), pp. 12-25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bentham Science Publishers Country of Publication: United Arab Emirates NLM ID: 100960527 Publication Model: Print Cited Medium: Print ISSN: 1389-2029 (Print) Linking ISSN: 13892029 NLM ISO Abbreviation: Curr Genomics Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: Saif Zone, Sharjah, U.A.E. : Bentham Science Publishers Original Publication: Hilversum, Netherlands ; Boca Raton, FL : Bentham Science Publishers, c2000- |
| مستخلص: | Background: Altered cytokine levels have been associated with poor outcomes among COVID-19 patients. TNF-α, IL-8 and IL-10 are key cytokines in COVID-19 pathogenesis, and CXCR-2 is a major chemokine receptor involved in inflammatory response. Polymorphisms in the genes of these proteins are proposed to influence disease outcomes. In this study, we aimed to find out the association of genetic polymorphisms in TNF-α, IL-8, IL-10 and CXCR-2 genes with susceptibility to and mortality of COVID-19. Methods: The present case-control study was conducted on 230 subjects, among whom 115 were clinically diagnosed and RT-PCR-confirmed COVID-19 patients and 115 healthy control subjects. The polymorphisms in TNFα -308 G>A (rs1800629), IL-8 -251T>A (rs4073), CXCR2 +785 C>T (rs2230054) genes were detected by ARMS -PCR assay whereas for IL-10 (-1082 G>A), rs1800896 G>A allele-specific PCR assay was used and their association with COVID-19 susceptibility and mortality was estimated by multivariate analysis. The results were analyzed for risk of infection and mortality through different inheritance models. Results: Frequencies of TNF-α rs1800629 GA, AA, IL-8 rs4073 TA, AA, IL-10 (-1082 G>A), rs1800896 GA and GG, and CXCR2 rs2230054 CT genotypes were significantly higher in COVID-19 patients compared to the control group ( p < 0.05). Furthermore, COVID-19 patients had a higher frequency of the polymorphic A allele of TNF-α, the A allele of IL-8, the G allele of IL-10, and the T allele of CXCR2. The risk of susceptibility to COVID-19 was significantly associated with TNF-α rs1800629 GA, GA+AA genotypes and the A allele, IL-8 rs4073 TA, AA genotypes and A allele, IL-10 rs1800872 GA and CC genotypes and C allele, and CXCR2 rs2230054 CT and CT+CC genotypes. TNF-α-GA and AA genotypes and A allele, IL-8 TA and AA genotypes and A allele and CXCR-2 CC and CT genotypes have significant associations with mortality risk in COVID-19 patients, while GA and GG genotypes of the IL-10 are shown to confer significant protection against mortality from COVID-19. Conclusion: The findings of this study provide important insights into the COVID-19 disease and susceptibility risk. The polymorphisms in TNFα -308 G>A (rs1800629), IL-8 -251T>A (rs4073), IL-10 (-1082 G>A), rs1800896 and CXCR2 +785 C>T (rs2230054) are associated with the risk of susceptibility to COVID-19 and with mortality in COVID-19 patients. Further studies with larger sample sizes are necessary to confirm our findings. Competing Interests: The authors declare no conflict of interest, financial or otherwise. (© 2024 Bentham Science Publishers.) |
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| فهرسة مساهمة: | Keywords: CXC chemokine receptor-2 (CXCR-2); Coronavirus disease-2019 (COVID-19); genetic polymorphisms; interleukin 10 (IL-10); interleukin 8 (IL-8); tumor necrosis factor alpha (TNF-α) |
| تواريخ الأحداث: | Date Created: 20240328 Latest Revision: 20240824 |
| رمز التحديث: | 20240826 |
| مُعرف محوري في PubMed: | PMC10964085 |
| DOI: | 10.2174/0113892029272497240103052359 |
| PMID: | 38544825 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1389-2029 |
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| DOI: | 10.2174/0113892029272497240103052359 |