دورية أكاديمية
أعرض في EDS

[Construction of mono/di-rhamnolipid ratios-manipulable strains and characterization of their corresponding surfactants' activity].

التفاصيل البيبلوغرافية
العنوان: [Construction of mono/di-rhamnolipid ratios-manipulable strains and characterization of their corresponding surfactants' activity].
المؤلفون: Zhao M; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.; University of Chinese Academy of Sciences, Beijing 100049, China., Zheng Y; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.; University of Chinese Academy of Sciences, Beijing 100049, China., Yu H; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China., Ma L; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.; University of Chinese Academy of Sciences, Beijing 100049, China.
المصدر: Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2024 Mar 25; Vol. 40 (3), pp. 786-798.
نوع المنشور: English Abstract; Journal Article
اللغة: Chinese
بيانات الدورية: Publisher: Ke xue chu ban she Country of Publication: China NLM ID: 9426463 Publication Model: Print Cited Medium: Internet ISSN: 1872-2075 (Electronic) Linking ISSN: 10003061 NLM ISO Abbreviation: Sheng Wu Gong Cheng Xue Bao Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Beijing : Ke xue chu ban she,
مواضيع طبية MeSH: Arabinose* , Glycolipids*/pharmacology, Anti-Bacterial Agents/pharmacology ; Surface-Active Agents/pharmacology ; Pseudomonas aeruginosa/genetics
مستخلص: Rhamnolipids (RLs) have emerged as one of the most promising classes of biosurfactants. The ratio of mono-RL to di-RL plays a significant role in determining its performance. Therefore, strains whose production of mono-RL and di-RL are manuplable, have advantage on applications in various scenarios. In this study, we developed a rhlC deletion mutant strain in Pseudomonas aeruginosa PAO1, which produced primarily mono-RL. Subsequently, we generated two complemented strains by integrating the arabinose-induced P BAD - rhlC gene, either directly into the chromosomes or expressing it on plasmids. Our results indicate that the ratio of mono-RL to di-RL synthesized by the complemented strain gradually decreased as the concentration of arabinose (the inducer) increased. Consequently, there was a decrease in emulsification ability and an increase in surface tension and critical micelle concentration (CMC) of the corresponding rhamnolipids. The complemented strains without inducer can produce a small amount of di-rhamnolipids, which enhanced the surfactant properties. Notably, the rhamnolipids induced by 0.10% arabinose exhibited the most potent antibacterial effect.
فهرسة مساهمة: Keywords: Pseudomonas aeruginosa PAO1; arabinose induction; ratio of mono- to di-rhamnolipid; rhamnosyltransferase Ⅱ (RhlC)
المشرفين على المادة: 0 (rhamnolipid)
B40ROO395Z (Arabinose)
0 (Glycolipids)
0 (Anti-Bacterial Agents)
0 (Surface-Active Agents)
تواريخ الأحداث: Date Created: 20240328 Date Completed: 20240329 Latest Revision: 20240329
رمز التحديث: 20240329
DOI: 10.13345/j.cjb.230413
PMID: 38545977
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-2075
DOI:10.13345/j.cjb.230413