دورية أكاديمية
Differences between cultured astrocytes from neonatal and adult Wistar rats: focus on in vitro aging experimental models.
| العنوان: | Differences between cultured astrocytes from neonatal and adult Wistar rats: focus on in vitro aging experimental models. |
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| المؤلفون: | Weber FB; Programa de Pós-Graduação Em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil., Santos CL; Programa de Pós-Graduação Em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil., da Silva A; Programa de Pós-Graduação Em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil., Schmitz I; Programa de Pós-Graduação Em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil., Rezena E; Programa de Pós-Graduação Em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil., Gonçalves CA; Programa de Pós-Graduação Em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil.; Programa de Pós-Graduação Em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Ramiro Barcelos, 2600, Porto Alegre, RS, 90035-003, Brazil.; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil., Quincozes-Santos A; Programa de Pós-Graduação Em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil.; Programa de Pós-Graduação Em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Ramiro Barcelos, 2600, Porto Alegre, RS, 90035-003, Brazil.; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil., Bobermin LD; Programa de Pós-Graduação Em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil. larissabobermin@gmail.com.; Programa de Pós-Graduação Em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Ramiro Barcelos, 2600, Porto Alegre, RS, 90035-003, Brazil. larissabobermin@gmail.com. |
| المصدر: | In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 2024 Apr; Vol. 60 (4), pp. 420-431. Date of Electronic Publication: 2024 Mar 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 9418515 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1543-706X (Electronic) Linking ISSN: 10712690 NLM ISO Abbreviation: In Vitro Cell Dev Biol Anim Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Berlin : Springer Original Publication: Columbia, MD : Tissue Culture Association, c1993- |
| مواضيع طبية MeSH: | Astrocytes*/metabolism , Animals, Newborn* , Rats, Wistar*, Animals ; Cells, Cultured ; Aging ; Reactive Oxygen Species/metabolism ; Rats ; Oxidative Stress ; Antioxidants/metabolism ; Glutamic Acid/metabolism ; Cellular Senescence ; Glucose/metabolism ; Glutamate-Ammonia Ligase/metabolism ; NF-kappa B/metabolism |
| مستخلص: | Astrocytes play key roles regulating brain homeostasis and accumulating evidence has suggested that glia are the first cells that undergo functional changes with aging, which can lead to a decline in brain function. In this context, in vitro models are relevant tools for studying aged astrocytes and, here, we investigated functional and molecular changes in cultured astrocytes obtained from neonatal or adult animals submitted to an in vitro model of aging by an additional period of cultivation of cells after confluence. In vitro aging induced different metabolic effects regarding glucose and glutamate uptake, as well as glutamine synthetase activity, in astrocytes obtained from adult animals compared to those obtained from neonatal animals. In vitro aging also modulated glutathione-related antioxidant defenses and increased reactive oxygen species and cytokine release especially in astrocytes from adult animals. Interestingly, in vitro aged astrocytes from adult animals exposed to pro-oxidant, inflammatory, and antioxidant stimuli showed enhanced oxidative and inflammatory responses. Moreover, these functional changes were correlated with the expression of the senescence marker p21, cytoskeleton markers, glutamate transporters, inflammatory mediators, and signaling pathways such as nuclear factor κB (NFκB)/nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1). Alterations in these genes are remarkably associated with a potential neurotoxic astrocyte phenotype. Therefore, considering the experimental limitations due to the need for long-term maintenance of the animals for studying aging, astrocyte cultures obtained from adult animals further aged in vitro can provide an improved experimental model for understanding the mechanisms associated with aging-related astrocyte dysfunction. (© 2024. The Society for In Vitro Biology.) |
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| فهرسة مساهمة: | Keywords: Aging; Glutamate homeostasis; In vitro astrocytes; Inflammatory response; Oxidative stress |
| المشرفين على المادة: | 0 (Reactive Oxygen Species) 0 (Antioxidants) 3KX376GY7L (Glutamic Acid) IY9XDZ35W2 (Glucose) EC 6.3.1.2 (Glutamate-Ammonia Ligase) 0 (NF-kappa B) |
| تواريخ الأحداث: | Date Created: 20240328 Date Completed: 20240506 Latest Revision: 20240506 |
| رمز التحديث: | 20240506 |
| DOI: | 10.1007/s11626-024-00896-1 |
| PMID: | 38546817 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1543-706X |
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| DOI: | 10.1007/s11626-024-00896-1 |