دورية أكاديمية
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Evaluation of nanoplastics-induced redox imbalance in cells, larval zebrafish, and daphnia magna with a superoxide anion radical fluorescent probe.

التفاصيل البيبلوغرافية
العنوان: Evaluation of nanoplastics-induced redox imbalance in cells, larval zebrafish, and daphnia magna with a superoxide anion radical fluorescent probe.
المؤلفون: Wang Y; CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China; University of Chinese Academy of Sciences, Beijing, 100049, China., Huang Y; CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China; School of Pharmacy, Binzhou Medical University, Yantai, 264003, China. Electronic address: yhuang@yic.ac.cn., Fu L; CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China., Wang X; CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China; School of Pharmacy, Binzhou Medical University, Yantai, 264003, China., Chen L; CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China; School of Pharmacy, Binzhou Medical University, Yantai, 264003, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, China. Electronic address: lxchen@yic.ac.cn.
المصدر: Chemosphere [Chemosphere] 2024 May; Vol. 356, pp. 141829. Date of Electronic Publication: 2024 Mar 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 0320657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1298 (Electronic) Linking ISSN: 00456535 NLM ISO Abbreviation: Chemosphere Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford : Elsevier Science Ltd
Original Publication: Oxford, New York, : Pergamon Press.
مواضيع طبية MeSH: Daphnia magna*/drug effects , Microplastics*/toxicity , Reactive Oxygen Species*/metabolism , Superoxides*/metabolism , Water Pollutants, Chemical*/toxicity , Zebrafish*, Animals ; Fluorescent Dyes/chemistry ; Larva/drug effects ; Larva/metabolism ; Nanoparticles/toxicity ; NF-kappa B/metabolism ; Oxidation-Reduction
مستخلص: Nanoplastics (NPs) is a novel plastic contaminant that could be taken up by cells and lead to severe biotoxicity toxicity, NPs in cells can cause oxidant damage by inducing reactive oxygen species (ROS) production and lead to acute inflammation. As a major ROS which related to many kinds of physiological and pathological processes, superoxide anion radical (O 2 •- ) could be utilized as a signal of oxidant damage effected by NPs exposure in vivo. To detect the toxic damage mechanism of NPs, a fluorescence probe Bcy-OTf has been developed to monitor O 2 •- fluctuations content in cells and aquatic organisms after exposure to NPs. The probe has a high sensitivity (LOD = 20 nM) and a rapid responsive time (within 6 min), and it has high selectivity and low cytotoxicity to analysis the levels of the endogenous O 2 •- . Endogenous O 2 •- induced by NPs in living cells, Daphnia magna and larval zebrafish were analyzed. Moreover, the results confirmed the key role of MAPK and NF-κB pathway in NPs stimulation mechanisms in cells. This study indicated that Bcy-OTf can precisely assess the fluctuations of endogenous O 2 •- , which has potential for applying in further analysis mechanisms of NPs biological risks.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Environmental pollutant; Fluorescent probe; Nanoplastics; Redox imbalance; Superoxide anion radical
المشرفين على المادة: 0 (Fluorescent Dyes)
0 (Microplastics)
0 (NF-kappa B)
0 (Reactive Oxygen Species)
11062-77-4 (Superoxides)
0 (Water Pollutants, Chemical)
تواريخ الأحداث: Date Created: 20240328 Date Completed: 20240430 Latest Revision: 20240801
رمز التحديث: 20240801
DOI: 10.1016/j.chemosphere.2024.141829
PMID: 38548081
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1298
DOI:10.1016/j.chemosphere.2024.141829