دورية أكاديمية
أعرض في EDS

Transcriptomic Analysis of Alzheimer's Disease Pathways in a Pakistani Population.

التفاصيل البيبلوغرافية
العنوان: Transcriptomic Analysis of Alzheimer's Disease Pathways in a Pakistani Population.
المؤلفون: Mondal T; Department of Biology, Howard University, Washington, DC, USA., Noreen Z; Department of Healthcare Biotechnology, National University of Sciences and Technology (NUST), Islamabad, Pakistan., Loffredo CA; Department of Oncology, Georgetown University, Washington, DC, USA., Johnson J; Department of Biology, Howard University, Washington, DC, USA., Bhatti A; Department of Healthcare Biotechnology, National University of Sciences and Technology (NUST), Islamabad, Pakistan., Nunlee-Bland G; Department of Pediatrics and Child Health, College of Medicine, Howard University, Washington, DC, USA., Quartey R; Department of Internal Medicine, College of Medicine, Howard University, Washington, DC, USA., Howell CD; Department of Internal Medicine, College of Medicine, Howard University, Washington, DC, USA., Moses G; Department of Biology, Howard University, Washington, DC, USA., Nnanabu T; Department of Biology, Howard University, Washington, DC, USA., Cotin ST; Department of Biology, Howard University, Washington, DC, USA., Clark M; Department of Biology, Howard University, Washington, DC, USA., Chandra V; Department of Neurology, Primus Hospital, New Delhi, India., Jana SS; Laboratory of Molecular and Cellular Biology (LMCB), Indian Association for the Cultivation of Science, Kolkata, India., Kwabi-Addo B; Department of Biochemistry, College of Medicine, Howard University, Washington, DC, USA., Korba BE; Department of Microbiology and Immunology, Georgetown University, Washington, DC, USA (retired, May 2022)., Shahzad S; Primary and Secondary Healthcare Department, Basic Health Unit, Union council Mundekey, Kasur, Pakistan., Bhatti MF; Department of Medicine, Pakistan Institute of Medical Sciences, Islamabad, Pakistan., Ghosh S; Department of Biology, Howard University, Washington, DC, USA.; Department of Pediatrics and Child Health, College of Medicine, Howard University, Washington, DC, USA.
المصدر: Journal of Alzheimer's disease reports [J Alzheimers Dis Rep] 2024 Mar 19; Vol. 8 (1), pp. 479-493. Date of Electronic Publication: 2024 Mar 19 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: IOS Press Country of Publication: Netherlands NLM ID: 101705500 Publication Model: eCollection Cited Medium: Internet ISSN: 2542-4823 (Electronic) Linking ISSN: 25424823 NLM ISO Abbreviation: J Alzheimers Dis Rep Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : IOS Press, [2017]-
مستخلص: Background: Alzheimer's disease (AD) is a multifactorial neurodegenerative disorder that is most prevalent in elderly individuals, especially in developed countries, and its prevalence is now increasing in developing countries like Pakistan.
Objective: Our goal was to characterize key genes and their levels of expression and related molecular transcriptome networks associated with AD pathogenesis in a pilot case-control study in a Pakistani population.
Methods: To obtain the spectrum of molecular networks associated with pathogenesis in AD patients in Pakistan (comparing cases and controls), we used high-throughput qRT-PCR (TaqMan Low-Density Array; n  = 33 subjects) coupled with Affymetrix Arrays ( n  = 8) and Ingenuity Pathway Analysis (IPA) to identify signature genes associated with Amyloid processing and disease pathways.
Results: We confirmed 16 differentially expressed AD-related genes, including maximum fold changes observed in CAPNS2 and CAPN1 . The global gene expression study observed that 61% and 39% of genes were significantly ( p -value 0.05) up- and downregulated, respectively, in AD patients compared to healthy controls. The key pathways include, e.g., Amyloid Processing , Neuroinflammation Signaling , and ErbB4 Signaling . The top-scoring networks in Diseases and Disorders Development were Neurological Disease , Organismal Injury and Abnormalities , and Psychological Disorders .
Conclusions: Our pilot study offers a non-invasive and efficient way of investigating gene expression patterns by combining TLDA and global gene expression method in AD patients by utilizing whole blood. This provides valuable insights into the expression status of genes related to Amyloid Processing , which could play potential role in future studies to identify sensitive, early biomarkers of AD in general.
Competing Interests: The authors have no conflict of interest to report.
(© 2024 – The authors. Published by IOS Press.)
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فهرسة مساهمة: Keywords: Alzheimer’s disease; amyloid processing; gene expression; pathogenesis; transcriptomic
تواريخ الأحداث: Date Created: 20240329 Latest Revision: 20240330
رمز التحديث: 20240330
مُعرف محوري في PubMed: PMC10977463
DOI: 10.3233/ADR-230146
PMID: 38549628
قاعدة البيانات: MEDLINE
الوصف
تدمد:2542-4823
DOI:10.3233/ADR-230146