دورية أكاديمية
miR-1202 regulates BPH-1 cell proliferation, apoptosis, and epithelial-to-mesenchymal transition through targeting HMGCL.
العنوان: | miR-1202 regulates BPH-1 cell proliferation, apoptosis, and epithelial-to-mesenchymal transition through targeting HMGCL. |
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المؤلفون: | Wang Z, Yin X, Yang P, Gong B, Liu H |
المصدر: | Acta biochimica et biophysica Sinica [Acta Biochim Biophys Sin (Shanghai)] 2024 May 25; Vol. 56 (5), pp. 675-687. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: China Science Publishing & Media Ltd Country of Publication: China NLM ID: 101206716 Publication Model: Print Cited Medium: Internet ISSN: 1745-7270 (Electronic) Linking ISSN: 16729145 NLM ISO Abbreviation: Acta Biochim Biophys Sin (Shanghai) Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2022- : Shanghai : China Science Publishing & Media Ltd. Original Publication: Shanghai : Shanghai Scientific and Technical Publishers, 2004- |
مواضيع طبية MeSH: | Apoptosis*/genetics , Apoptosis*/drug effects , Cell Proliferation*/genetics , Cell Proliferation*/drug effects , Epithelial-Mesenchymal Transition*/genetics , Epithelial-Mesenchymal Transition*/drug effects , MicroRNAs*/genetics , MicroRNAs*/metabolism , Prostatic Hyperplasia*/pathology , Prostatic Hyperplasia*/metabolism , Prostatic Hyperplasia*/genetics, Animals ; Humans ; Male ; Rats ; Cell Line ; Transforming Growth Factor beta/metabolism ; Wnt Signaling Pathway/genetics ; Wnt Signaling Pathway/drug effects |
مستخلص: | Benign prostatic hyperplasia (BPH) is the expansion of the prostate gland that results in urinary symptoms. Both the epithelial-to-mesenchymal transition (EMT) and the Wnt signaling pathway are associated with BPH pathology. In this study, we find that miR-1202 is increased in BPH samples. Overexpression of miR-1202 in TGF-β-treated BPH-1 cells enhances cell survival and DNA synthesis and inhibits cell apoptosis, whereas miR-1202 inhibition partially abolishes the effects of TGF-β on BPH-1 cells. miR-1202 overexpression reduces E-cadherin level but elevates vimentin, N-cadherin, and snail levels, whereas miR-1202 inhibition partially attenuates the effects of TGF-β on EMT markers. Regarding the Wnt/β-catenin pathway, miR-1202 overexpression significantly enhances, whereas miR-1202 inhibition partially decreases, the promotive effects of TGF-β on Wnt1, c-Myc, and cyclin D1 proteins. 3-Hydroxy-3-methylglutaryl-CoA lyase (HMGCL) is a direct downstream target of miR-1202, and miR-1202 inhibits HMGCL expression through binding to its 3'UTR. Overexpression of HMGCL significantly reduces the effect of miR-1202 overexpression on the phenotypes of BPH-1 cells by inhibiting cell survival and promoting apoptosis. Similarly, HMGCL overexpression has the opposite effects on EMT markers and the Wnt/β-catenin signaling, and markedly alleviates the effects of miR-1202 overexpression. Finally, in the BPH rat model, Ki67 and vimentin levels are elevated, but E-cadherin and HMGCL levels are reduced. In conclusion, miR-1202 is upregulated in benign prostatic hyperplasia; miR-1202 enhances epithelial cell proliferation, suppresses cell apoptosis, and promotes EMT by targeting HMGCL. The Wnt/β-catenin pathway may participate in the miR-1202/HMGCL axis-mediated regulation of BPH-1 cell phenotypes. |
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فهرسة مساهمة: | Keywords: 3-hydroxy-3-methylglutaryl-CoA lyase (HMGCL); Wnt/β-catenin pathway; benign prostate hyperplasia (BPH); epithelial-to-mesenchymal transition (EMT); miR-1202 |
المشرفين على المادة: | 0 (MicroRNAs) 0 (Transforming Growth Factor beta) EC 4.1.3.4 (3-hydroxy-3-methylglutaryl-coenzyme A lyase) |
تواريخ الأحداث: | Date Created: 20240329 Date Completed: 20240530 Latest Revision: 20240616 |
رمز التحديث: | 20240616 |
مُعرف محوري في PubMed: | PMC11177111 |
DOI: | 10.3724/abbs.2024001 |
PMID: | 38551020 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1745-7270 |
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DOI: | 10.3724/abbs.2024001 |