دورية أكاديمية
MEK signalling pathway is required for hypoblast specification and migration in ovine.
| العنوان: | MEK signalling pathway is required for hypoblast specification and migration in ovine. |
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| المؤلفون: | Martínez de Los Reyes N; Department of Animal Reproduction, INIA, CSIC, Madrid, Spain., Flores-Borobia I; Department of Animal Reproduction, INIA, CSIC, Madrid, Spain., Carvajal-Serna M; Department of Animal Reproduction, INIA, CSIC, Madrid, Spain., Marigorta P; Department of Animal Reproduction, INIA, CSIC, Madrid, Spain., Bermejo-Álvarez P; Department of Animal Reproduction, INIA, CSIC, Madrid, Spain., Ramos-Ibeas P; Department of Animal Reproduction, INIA, CSIC, Madrid, Spain. |
| المصدر: | Reproduction (Cambridge, England) [Reproduction] 2024 Apr 29; Vol. 167 (6). Date of Electronic Publication: 2024 Apr 29 (Print Publication: 2024). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for the Society for Reproduction and Fertility by BioScientifica Country of Publication: England NLM ID: 100966036 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1741-7899 (Electronic) Linking ISSN: 14701626 NLM ISO Abbreviation: Reproduction Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004->: Bristol, UK : Published for the Society for Reproduction and Fertility by BioScientifica Original Publication: Cambridge, UK : Journals of Reproduction and Fertility, Ltd. c2001- |
| مواضيع طبية MeSH: | Cell Differentiation* , Cell Movement* , Embryonic Development* , MAP Kinase Signaling System*, Animals ; Female ; Pregnancy ; Blastocyst/metabolism ; Blastocyst/cytology ; Blastocyst Inner Cell Mass/metabolism ; Blastocyst Inner Cell Mass/cytology ; Cell Lineage ; Sheep ; Signal Transduction ; Mice |
| مستخلص: | In Brief: MEK signalling pathway is required for hypoblast differentiation in mouse embryos, but its role in ungulate embryos remains controversial. This paper demonstrates that MEK is required for hypoblast specification in the inner cell mass of the ovine blastocyst and that it plays a role during the hypoblast migration occurring following blastocyst hatching. Abstract: Early embryo development requires the differentiation of three cell lineages in two differentiation events. The second lineage specification differentiates the inner cell mass into epiblast, which will form the proper fetus, and hypoblast, which together with the trophectoderm will form the extraembryonic membranes and the fetal part of the placenta. MEK signalling pathway is required for hypoblast differentiation in mouse embryos, but its role in ungulate embryos remains controversial. The aim of this work was to analyse the role of MEK signalling on hypoblast specification at the blastocyst stage and on hypoblast migration during post-hatching stages in vitro in the ovine species. Using well-characterized and reliable lineage markers, and different MEK inhibitor concentrations, we demonstrate that MEK signalling pathway is required for hypoblast specification in the inner cell mass of the ovine blastocyst, and that it plays a role during the hypoblast migration occurring following blastocyst hatching. These results show that the role of MEK signalling pathway on hypoblast specification is conserved in phylogenetically distant mammals. |
| تواريخ الأحداث: | Date Created: 20240329 Date Completed: 20240429 Latest Revision: 20240501 |
| رمز التحديث: | 20240501 |
| DOI: | 10.1530/REP-24-0003 |
| PMID: | 38552319 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1741-7899 |
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| DOI: | 10.1530/REP-24-0003 |