دورية أكاديمية
Identification of immunity-related genes distinctly regulated by Manduca sexta Spӓtzle-1/2 and Escherichia coli peptidoglycan.
العنوان: | Identification of immunity-related genes distinctly regulated by Manduca sexta Spӓtzle-1/2 and Escherichia coli peptidoglycan. |
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المؤلفون: | Miao Z; Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA., Xiong C; Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA., Wang Y; Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA., Shan T; Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA., Jiang H; Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA. Electronic address: haobo.jiang@okstate.edu. |
المصدر: | Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2024 May; Vol. 168, pp. 104108. Date of Electronic Publication: 2024 Mar 27. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 9207282 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0240 (Electronic) Linking ISSN: 09651748 NLM ISO Abbreviation: Insect Biochem Mol Biol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: <2003->: Amsterdam : Elsevier Science Original Publication: Oxford [England] ; New York : Pergamon Press, c1992- |
مواضيع طبية MeSH: | Manduca*/metabolism , Cecropins*/metabolism, Animals ; Escherichia coli/genetics ; Peptidoglycan ; Insect Proteins/metabolism ; Cytokines/metabolism ; Drosophila/metabolism |
مستخلص: | The immune system of Manduca sexta has been well studied to understand molecular mechanisms of insect antimicrobial responses. While evidence supports the existence of major immune signaling pathways in this species, it is unclear how induced production of defense proteins is specifically regulated by the Toll and Imd pathways. Our previous studies suggested that diaminopimelic acid-type peptidoglycans (DAP-PG) from Gram-negative and some Gram-positive bacteria, more than Lys-type peptidoglycans (Lys-PG) from other Gram-positive bacteria, triggers both pathways through membrane-bound receptors orthologous to Drosophila Toll and PGRP-LC. In this study, we produced M. sexta proSpätzle-1 and proSpätzle-2 in Sf9 cells, identified their processing enzymes, and used prophenoloxidase activating protease-3 to activate the cytokine precursors. After Spätzle-1 and -2 were isolated from the reaction mixtures, we separately injected the purified cytokines into larval hemocoel to induce gene transcription in fat body through the Toll pathway solely. On the other hand, we treated a M. sexta cell line with E. coli DAP-PG to only induce the Imd pathway and target gene expression. RNA-Seq analysis of the fat body and cultured cells collected at 0, 6, and 24 h after treatment indicated that expression of diapausin-4, -10, -12, -13, cecropin-2, -4, -5, attacin-5, -11, and lebocin D is up-regulated predominantly via Toll signaling, whereas transcription of cecropin-6, gloverin, lysozyme-1, and gallerimycin-2 is mostly induced by DAP-PG via Imd signaling. Other antimicrobial peptides are expressed in response to both pathways. Transcripts of most Toll-specific genes (e.g., lebocin D) peaked at 6 h, contrasting the gradual increase and plateauing of drosomycin mRNA level at 24-48 h in Drosophila. We also used T (oll)-I (md) ratios to estimate relative contributions of the two pathways to transcriptional regulation of other components of the immune system. The differences in pathway specificity and time course of transcriptional regulation call for further investigations in M. sexta and other insects. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
فهرسة مساهمة: | Keywords: Fat body; Hemolymph proteins; Imd pathway; Insect immunity; RNA-Seq; Toll pathway |
المشرفين على المادة: | 0 (Peptidoglycan) 0 (Cecropins) 0 (Insect Proteins) 0 (Cytokines) |
تواريخ الأحداث: | Date Created: 20240329 Date Completed: 20240417 Latest Revision: 20240417 |
رمز التحديث: | 20240417 |
DOI: | 10.1016/j.ibmb.2024.104108 |
PMID: | 38552808 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1879-0240 |
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DOI: | 10.1016/j.ibmb.2024.104108 |