دورية أكاديمية
Identification of citrullinated α1-antitrypsin (A1AT) in saliva in a mouse model of rheumatoid arthritis.
| العنوان: | Identification of citrullinated α1-antitrypsin (A1AT) in saliva in a mouse model of rheumatoid arthritis. |
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| المؤلفون: | Sakaguchi W; Department of Pathology and Histomorphology, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan. Electronic address: sakaguchi@kdu.ac.jp., Saruta J; Department of Education Planning, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan. Electronic address: saruta@kdu.ac.jp., Yamamoto Y; Department of Dental Hygiene, Kanagawa Dental University, Junior College, 82 Inaoka, Yokosuka, Kanagawa 2388580, Japan. Electronic address: yamamoto.yuko@kdu.ac.jp., Shimizu T; Department of Implantology and Periodontology, Kanagawa Dental University, 3-31-6 Tsuruya, Kanagawa-ku, Yokohama 2210835, Kanagawa, Japan. Electronic address: shimizu@kdu.ac.jp., Fuchida S; Department of Education Planning, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan. Electronic address: fuchida@kdu.ac.jp., Tsukinoki K; Department of Pathology and Histomorphology, Kanagawa Dental University, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan. Electronic address: tsukinoki@kdu.ac.jp. |
| المصدر: | Journal of oral biosciences [J Oral Biosci] 2024 Jun; Vol. 66 (2), pp. 473-482. Date of Electronic Publication: 2024 Mar 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101226721 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1880-3865 (Electronic) Linking ISSN: 13490079 NLM ISO Abbreviation: J Oral Biosci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2012- : Amsterdam : Elsevier B.V. Original Publication: Tokyo : Japanese Association for Oral Biology, [2004]- |
| مواضيع طبية MeSH: | alpha 1-Antitrypsin*/metabolism , Arthritis, Rheumatoid*/metabolism , Arthritis, Rheumatoid*/pathology , Saliva*/chemistry , Saliva*/metabolism , Disease Models, Animal* , Blotting, Western* , Enzyme-Linked Immunosorbent Assay* , Mice, Inbred DBA*, Animals ; Mice ; Biomarkers/metabolism ; Proteomics ; Citrullination ; Ankle Joint/pathology |
| مستخلص: | Objectives: Rheumatoid arthritis (RA) is an autoimmune disease characterized by progressive joint destruction. Early diagnosis and treatment, before joint deformation or destruction occurs, are crucial. Identifying novel biomarkers for RA in saliva could potentially enable early detection of the disease, prior to its onset. Methods: We conducted a comprehensive proteomic analysis of salivary proteins in a mouse model of RA. Proteins were identified using western blotting and enzyme-linked immunosorbent assay in the serum, saliva, and ankle joints of DBA/1JJmsSlc mice, a model of RA. Ankle joints and submandibular glands were stained with hematoxylin and eosin and immunostained, and the results were compared with those of control mice. Results: Citrullinated alpha-1 antitrypsin (A1AT, 46 kDa) was commonly detected in the saliva, serum, and ankle joints of mice with severe RA and was confirmed through proteomic analysis. Western blotting showed a band corresponding to 46 kDa in the serum, saliva, and ankle joints. Immunostaining of the ankle joints with the A1AT antibody showed a strong positive signal in the synovium. Conclusions: In DBA/1JJmsSlc mice, cyclic citrullinated peptide antibodies and A1AT may be involved in citrullination and contribute to the development and severity of RA, making them valuable treatment targets requiring further study. Competing Interests: Declaration of competing interest All authors are required to disclose any COI within the period of 12 months prior to the submission of any manuscript in the subject matter of which any company, entity, or organization has an interest. (Copyright © 2024 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: A1AT; DBA/1JJmsSlc mice; Proteomic analysis; RA; Saliva |
| المشرفين على المادة: | 0 (alpha 1-Antitrypsin) 0 (Biomarkers) |
| تواريخ الأحداث: | Date Created: 20240330 Date Completed: 20240613 Latest Revision: 20240627 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1016/j.job.2024.03.007 |
| PMID: | 38554831 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1880-3865 |
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| DOI: | 10.1016/j.job.2024.03.007 |