دورية أكاديمية
Diagnosis of thrombotic thrombocytopenic purpura: easy-to-use fiber optic surface plasmon resonance immunoassays for automated ADAMTS-13 antigen and conformation evaluation.
| العنوان: | Diagnosis of thrombotic thrombocytopenic purpura: easy-to-use fiber optic surface plasmon resonance immunoassays for automated ADAMTS-13 antigen and conformation evaluation. |
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| المؤلفون: | Bonnez Q; Laboratory for Thrombosis Research, IRF Life Sciences, KU Leuven Campus Kulak Kortrijk, Kortrijk, Belgium. Electronic address: https://twitter.com/BonnezQuintijn., Dekimpe C; Laboratory for Thrombosis Research, IRF Life Sciences, KU Leuven Campus Kulak Kortrijk, Kortrijk, Belgium., Bekaert T; Laboratory for Thrombosis Research, IRF Life Sciences, KU Leuven Campus Kulak Kortrijk, Kortrijk, Belgium., Tellier E; Institut National de la Santé et de la Recherche Médicale, INRAE, C2VN, Aix-Marseille University, Marseille, France., Kaplanski G; Institut National de la Santé et de la Recherche Médicale, INRAE, C2VN, Aix-Marseille University, Marseille, France; Service de Médecine Interne et Immunologie Clinique, CHU Conception, Aix-Marseille University, APHM, Marseille, France., Joly BS; Service d'Hématologie Biologique Hôpital Lariboisière, AP-HP and EA3518, IRSL, Université Paris Cité, Paris, France., Veyradier A; Service d'Hématologie Biologique Hôpital Lariboisière, AP-HP and EA3518, IRSL, Université Paris Cité, Paris, France; Centre de Référence des Microangiopathies Thrombotiques, AP-HP, Paris, France., Coppo P; Department of Hematology, Reference Center for Thrombotic Microangiopathies, Saint-Antoine University Hospital, AP-HP, Paris, France., Lammertyn J; Department of Biosystems, Biosensors Group, KU Leuven, Leuven, Belgium., Tersteeg C; Laboratory for Thrombosis Research, IRF Life Sciences, KU Leuven Campus Kulak Kortrijk, Kortrijk, Belgium., De Meyer SF; Laboratory for Thrombosis Research, IRF Life Sciences, KU Leuven Campus Kulak Kortrijk, Kortrijk, Belgium., Vanhoorelbeke K; Laboratory for Thrombosis Research, IRF Life Sciences, KU Leuven Campus Kulak Kortrijk, Kortrijk, Belgium. Electronic address: Karen.Vanhoorelbeke@kuleuven.be. |
| المصدر: | Journal of thrombosis and haemostasis : JTH [J Thromb Haemost] 2024 Jul; Vol. 22 (7), pp. 1936-1946. Date of Electronic Publication: 2024 Mar 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 101170508 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1538-7836 (Electronic) Linking ISSN: 15387836 NLM ISO Abbreviation: J Thromb Haemost Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [New York] : Elsevier Original Publication: Oxford : Blackwell Pub. |
| مواضيع طبية MeSH: | ADAMTS13 Protein*/blood , ADAMTS13 Protein*/immunology , Purpura, Thrombotic Thrombocytopenic*/diagnosis , Purpura, Thrombotic Thrombocytopenic*/blood , Purpura, Thrombotic Thrombocytopenic*/immunology , Surface Plasmon Resonance* , Enzyme-Linked Immunosorbent Assay*/methods, Humans ; Case-Control Studies ; Biomarkers/blood ; Reproducibility of Results ; Protein Conformation ; Predictive Value of Tests ; Immunoassay/methods ; Automation, Laboratory ; Female ; Male |
| مستخلص: | Background: Laboratory diagnosis of immune-mediated thrombotic thrombocytopenic purpura (iTTP) remains challenging when ADAMTS-13 activity ranges between 10% and 20%. To prevent misdiagnosis, open ADAMTS-13 conformation gained clinical attention as a novel biomarker, especially to diagnose acute iTTP in patients with diagnostic undecisive ADAMTS-13 activity. Plasma ADAMTS-13 conformation analysis corrects for ADAMTS-13 antigen, with both parameters being characterized in enzyme-linked immunosorbent assay (ELISA)-based reference assays requiring expert technicians. Objectives: To design ADAMTS-13 antigen and conformation assays on automated, easy-to-use fiber optic surface plasmon resonance (FO-SPR) technology to promote assay accessibility and diagnose challenging iTTP patients. Methods: ADAMTS-13 antigen and conformation assays were designed on FO-SPR technology. Plasma of 20 healthy donors and 20 acute iTTP patients were quantified, and data from FO-SPR and ELISA reference assays were compared. Results: Following assay design, both antigen and conformation FO-SPR assays were optimized and characterized, presenting strong analytical sensitivity (detection limit of 0.001 μg/mL) and repeatability (interassay variation of 14.4%). Comparative analysis suggested positive correlation (Spearman r of 0.92) and good agreement between FO-SPR and ELISA assays. As expected, FO-SPR assays showed a closed or open ADAMTS-13 conformation in healthy donors and acute iTTP patients, respectively. Conclusion: Both ADAMTS-13 antigen and conformation assays were transferred onto automated, easy-to-use FO-SPR technology, displaying potent analytical sensitivity and reproducibility. ADAMTS-13 antigen and conformation were determined for healthy donors and acute iTTP patients showing strong correlation with ELISA reference. Introducing FO-SPR technology in clinical context could support routine diagnosis of acute iTTP patients, notably when ADAMTS-13 activity fluctuates between 10% and 20%. Competing Interests: Declaration of competing interests J.L. is a member of the Board of Directors of FOx Biosystems. Q.B., C.D., T.B., E.T., G.K., B.S.J., A.V., P.C., C.T., S.F.D.M., and K.V. have no conflicts of interest to disclose. (Copyright © 2024 International Society on Thrombosis and Haemostasis. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: ADAMTS-13 protein; enzyme immunoassay; laboratory diagnosis; purpura; surface plasmon resonance; thrombotic thrombocytopenic |
| المشرفين على المادة: | EC 3.4.24.87 (ADAMTS13 Protein) EC 3.4.24.87 (ADAMTS13 protein, human) 0 (Biomarkers) |
| تواريخ الأحداث: | Date Created: 20240330 Date Completed: 20240630 Latest Revision: 20240722 |
| رمز التحديث: | 20240722 |
| DOI: | 10.1016/j.jtha.2024.03.012 |
| PMID: | 38554935 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1538-7836 |
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| DOI: | 10.1016/j.jtha.2024.03.012 |