دورية أكاديمية
أعرض في EDS

Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation.

التفاصيل البيبلوغرافية
العنوان: Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation.
المؤلفون: Catandi GD; Equine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, 3101 Rampart Road, Fort Collins, CO, 80521, USA.; Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA.; Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK, 74078, USA., Fresa KJ; Equine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, 3101 Rampart Road, Fort Collins, CO, 80521, USA.; Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA., Cheng MH; Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, 80523, USA., Whitcomb LA; Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA., Broeckling CD; Proteomics and Metabolomics Facility, Colorado State University, Fort Collins, CO, 80523, USA., Chen TW; Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, 80523, USA.; School of Biomedical Engineering, Colorado State University, Fort Collins, CO, 80523, USA., Chicco AJ; Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA., Carnevale EM; Equine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, 3101 Rampart Road, Fort Collins, CO, 80521, USA. elaine.carnevale@colostate.edu.; Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA. elaine.carnevale@colostate.edu.
المصدر: Scientific reports [Sci Rep] 2024 Mar 30; Vol. 14 (1), pp. 7571. Date of Electronic Publication: 2024 Mar 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Ovarian Follicle*/metabolism , Oocytes*/metabolism, Humans ; Horses ; Animals ; Female ; Follicular Fluid ; Obesity/metabolism ; Lipids ; Dietary Supplements
مستخلص: Obesity is a growing concern in human and equine populations, predisposing to metabolic pathologies and reproductive disturbances. Cellular lipid accumulation and mitochondrial dysfunction play an important role in the pathologic consequences of obesity, which may be mitigated by dietary interventions targeting these processes. We hypothesized that obesity in the mare promotes follicular lipid accumulation and altered mitochondrial function of oocytes and granulosa cells, potentially contributing to impaired fertility in this population. We also predicted that these effects could be mitigated by dietary supplementation with a combination of targeted nutrients to improve follicular cell metabolism. Twenty mares were grouped as: Normal Weight [NW, n = 6, body condition score (BCS) 5.7 ± 0.3], Obese (OB, n = 7, BCS 7.7 ± 0.2), and Obese Diet Supplemented (OBD, n = 7, BCS 7.7 ± 0.2), and fed specific feed regimens for ≥ 6 weeks before sampling. Granulosa cells, follicular fluid, and cumulus-oocyte complexes were collected from follicles ≥ 35 mm during estrus and after induction of maturation. Obesity promoted several mitochondrial metabolic disturbances in granulosa cells, reduced L-carnitine availability in the follicle, promoted lipid accumulation in cumulus cells and oocytes, and increased basal oocyte metabolism. Diet supplementation of a complex nutrient mixture mitigated most of the metabolic changes in the follicles of obese mares, resulting in parameters similar to NW mares. In conclusion, obesity disturbs the equine ovarian follicle by promoting lipid accumulation and altering mitochondrial function. These effects may be partially mitigated with targeted nutritional intervention, thereby potentially improving fertility outcomes in the obese female.
(© 2024. The Author(s).)
References: Biol Trace Elem Res. 2020 Feb;193(2):334-341. (PMID: 30977089)
Biol Reprod. 2015 Jan;92(1):16. (PMID: 25297544)
Methods Mol Biol. 2019;1978:121-135. (PMID: 31119660)
Biosens Bioelectron. 2019 May 15;133:39-47. (PMID: 30909011)
Biol Reprod. 2016 Jan;94(1):23. (PMID: 26658709)
Vet Clin North Am Equine Pract. 2016 Dec;32(3):379-399. (PMID: 27726987)
J Vet Intern Med. 2012 Nov-Dec;26(6):1413-8. (PMID: 22946995)
J Nutr. 2017 Dec;147(12):2212-2219. (PMID: 29021369)
J Biol Chem. 2009 Aug 21;284(34):22840-52. (PMID: 19553674)
Biol Reprod. 2022 Feb 22;106(2):338-350. (PMID: 34918035)
Hum Reprod Update. 2021 Jan 4;27(1):27-47. (PMID: 33020823)
Anal Biochem. 2014 Feb 1;446:64-8. (PMID: 24161612)
Development. 2015 Feb 15;142(4):681-91. (PMID: 25670793)
Diabetes. 2013 Jan;62(1):1-8. (PMID: 23258903)
BMC Bioinformatics. 2008 Nov 28;9:504. (PMID: 19040729)
Taiwan J Obstet Gynecol. 2017 Apr;56(2):159-164. (PMID: 28420500)
Semin Reprod Med. 2009 Jan;27(1):32-42. (PMID: 19197803)
Anal Chem. 2006 Feb 1;78(3):779-87. (PMID: 16448051)
Reprod Fertil Dev. 2022 Dec;35(2):1-18. (PMID: 36592978)
Am J Physiol Regul Integr Comp Physiol. 2020 May 1;318(5):R972-R980. (PMID: 32233925)
Reproduction. 2014 Jul;148(1):R15-27. (PMID: 24760880)
J Ovarian Res. 2020 Jan 6;13(1):4. (PMID: 31907049)
Obstet Gynecol. 2011 Jul;118(1):63-70. (PMID: 21691164)
Placenta. 2018 Apr;64:44-52. (PMID: 29626980)
Reproduction. 2022 Jan 28;163(4):183-198. (PMID: 37379450)
Antioxidants (Basel). 2021 Apr 13;10(4):. (PMID: 33924341)
PLoS One. 2012;7(11):e49217. (PMID: 23152876)
J Diabetes Sci Technol. 2009 Jan;3(1):163-74. (PMID: 20046661)
Int J Obes Suppl. 2019 Apr;9(1):65-72. (PMID: 31391925)
Reprod Fertil Dev. 2009;21(3):419-27. (PMID: 19261219)
J Reprod Immunol. 2018 Nov;130:25-29. (PMID: 30174020)
Reprod Fertil Dev. 2009;21(4):615-23. (PMID: 19383268)
J Lipid Res. 2014 Apr;55(4):635-44. (PMID: 24395925)
Reprod Biol Endocrinol. 2014 Feb 05;12:13. (PMID: 24498875)
PLoS One. 2010 Apr 09;5(4):e10074. (PMID: 20404917)
Biol Reprod. 2013 Jun 27;88(6):164. (PMID: 23616596)
Reprod Domest Anim. 2008 Oct;43(5):623-32. (PMID: 18384498)
Nutrients. 2021 May 28;13(6):. (PMID: 34071499)
Curr Biol. 2018 Apr 2;28(7):1124-1131.e3. (PMID: 29576478)
Biol Reprod. 2016 Jan;94(1):15. (PMID: 26632608)
Mol Hum Reprod. 2015 Jan;21(1):23-30. (PMID: 24923276)
Reproduction. 2013 Jan 08;145(1):33-44. (PMID: 23108110)
Cell Metab. 2008 Jan;7(1):45-56. (PMID: 18177724)
J Equine Vet Sci. 2019 Aug;79:131-138. (PMID: 31405493)
Equine Vet J. 1983 Oct;15(4):371-2. (PMID: 6641685)
Vet J. 2009 Feb;179(2):204-10. (PMID: 18440844)
Fertil Steril. 2012 Jun;97(6):1438-43. (PMID: 22440252)
Endocrinology. 2010 Nov;151(11):5438-45. (PMID: 20861227)
Hum Reprod. 2016 Sep;31(9):2090-7. (PMID: 27432748)
Theriogenology. 2017 Oct 1;101:15-25. (PMID: 28708512)
Reproduction. 2021 Apr;161(4):399-409. (PMID: 33539317)
Nucleic Acids Res. 2001 May 1;29(9):e45. (PMID: 11328886)
Nutrients. 2023 Jan 25;15(3):. (PMID: 36771318)
Cell Tissue Res. 1997 Apr;288(1):119-26. (PMID: 9042779)
Biol Trace Elem Res. 2018 Sep;185(1):48-55. (PMID: 29307112)
J Assist Reprod Genet. 2012 Nov;29(11):1289-97. (PMID: 22968515)
Reprod Fertil Dev. 2006;18(6):609-17. (PMID: 16930507)
Animals (Basel). 2021 Aug 04;11(8):. (PMID: 34438761)
Vet Immunol Immunopathol. 2012 Jan 15;145(1-2):283-9. (PMID: 22169327)
Theriogenology. 2008 Jan 1;69(1):23-30. (PMID: 17976712)
Reprod Biol Endocrinol. 2018 Mar 9;16(1):22. (PMID: 29523133)
J Equine Vet Sci. 2020 Jun;89:103097. (PMID: 32563445)
Adv Nutr. 2021 Dec 1;12(6):2372-2386. (PMID: 34139003)
Biol Reprod. 2004 Oct;71(4):1195-201. (PMID: 15189824)
Reprod Biol Endocrinol. 2013 Jul 17;11:67. (PMID: 23866102)
J Anim Sci. 2004 Aug;82(8):2321-8. (PMID: 15318731)
Sci Rep. 2020 Jun 17;10(1):9806. (PMID: 32555236)
Anal Chem. 2014 Jul 15;86(14):6812-7. (PMID: 24927477)
J Hematol Oncol. 2013 Feb 25;6:19. (PMID: 23442817)
PLoS One. 2018 Jan 26;13(1):e0190309. (PMID: 29373573)
Cell Metab. 2012 May 2;15(5):764-77. (PMID: 22560225)
PLoS One. 2017 Jan 12;12(1):e0169295. (PMID: 28081146)
J Equine Vet Sci. 2020 Jun;89:103022. (PMID: 32563447)
J Lipid Res. 2016 Nov;57(11):1976-1986. (PMID: 27009116)
Hum Reprod. 2012 Dec;27(12):3531-9. (PMID: 23019302)
Vet J. 2021 Apr;270:105627. (PMID: 33641803)
J Clin Endocrinol Metab. 2015 May;100(5):1845-54. (PMID: 25695883)
Reprod Fertil Dev. 2015 May;27(4):593-601. (PMID: 25751151)
معلومات مُعتمدة: Grant No. COLV 2021-09 / Project Accession No. 1026913 USDA National Institute of Food and Agriculture Animal Health and Disease
المشرفين على المادة: 0 (Lipids)
تواريخ الأحداث: Date Created: 20240330 Date Completed: 20240401 Latest Revision: 20240403
رمز التحديث: 20240403
مُعرف محوري في PubMed: PMC10981747
DOI: 10.1038/s41598-024-58323-0
PMID: 38555310
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-58323-0