دورية أكاديمية
Protocol for generating mouse morula-like cells resembling 8- to 16-cell stage embryo cells.
| العنوان: | Protocol for generating mouse morula-like cells resembling 8- to 16-cell stage embryo cells. |
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| المؤلفون: | Li H; Guangzhou Medical University, Panyu District, Guangzhou 511495, Guangdong Province, China; Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou 510005, Guangdong Province, China. Electronic address: li_huanhuan@gzlab.ac.cn., Chang L; Guangzhou Medical University, Panyu District, Guangzhou 511495, Guangdong Province, China; Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou 510005, Guangdong Province, China., Huang J; Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou 510005, Guangdong Province, China; Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China., Silva JCR; Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou 510005, Guangdong Province, China. Electronic address: jose_silva@gzlab.ac.cn. |
| المصدر: | STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 102934. Date of Electronic Publication: 2024 Mar 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Morula*/cytology, Animals ; Mice ; Embryo, Mammalian/cytology ; Blastocyst/cytology ; Blastocyst/metabolism ; Embryonic Development/physiology ; Cell Culture Techniques/methods ; Female ; Cell Differentiation/physiology |
| مستخلص: | Generating cell types with properties of embryo cells with full developmental potential is of great biological importance. Here, we present a protocol for generating mouse morula-like cells (MLCs) resembling 8- to 16-cell stage embryo cells. We describe steps for induction, via increasing Stat3 activation, and the isolation of MLCs. We then detail procedures for segregating MLCs into blastocyst cell fates and how to create embryo-like structures from them. This system provides a stem-cell-based embryo model to study early embryo development. For complete details on the use and execution of this protocol, please refer to Li et al. 1 . Competing Interests: Declaration of interests J.C.R.S. and H.L. submitted a patent application (202210437013.X) related to this work (filed by Guangzhou National Laboratory). (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Developmental biology; Organoids; Stem Cells |
| تواريخ الأحداث: | Date Created: 20240331 Date Completed: 20240625 Latest Revision: 20240625 |
| رمز التحديث: | 20240625 |
| مُعرف محوري في PubMed: | PMC10998243 |
| DOI: | 10.1016/j.xpro.2024.102934 |
| PMID: | 38555588 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2024.102934 |