دورية أكاديمية
Peritoneal carcinomatosis in mouse models.
| العنوان: | Peritoneal carcinomatosis in mouse models. |
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| المؤلفون: | Arrizabalaga L; Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain. Electronic address: larrizabala@unav.es., Di Trani CA; Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain., Risson A; Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain., Belsúe V; Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain., Gomar C; Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain., Ardaiz N; Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain., Berrondo P; Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain; Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Madrid, Spain., Aranda F; Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain. Electronic address: faranda@unav.es., Bella Á; Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain. Electronic address: abellacarre@unav.es. |
| المصدر: | Methods in cell biology [Methods Cell Biol] 2024; Vol. 185, pp. 67-78. Date of Electronic Publication: 2024 Mar 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0373334 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0091-679X (Print) Linking ISSN: 0091679X NLM ISO Abbreviation: Methods Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Academic Press |
| مواضيع طبية MeSH: | Peritoneal Neoplasms*/drug therapy , Peritoneal Neoplasms*/pathology , Peritoneal Neoplasms*/secondary, Mice ; Female ; Animals ; Disease Models, Animal |
| مستخلص: | Peritoneal carcinomatosis (PCa) represents a metastatic stage of a disease with unmet therapeutic options. Malignant cells from primary tumors (gastrointestinal or gynecologic malignancies) invade the peritoneal cavity and eventually seed onto peritoneal surfaces, with the omentum being the most common nest area. With a median survival of less than 6 months, PCa has a dismal prognosis that can be improved with treatments only available to a select few individuals with low tumor burden. Thus, the discovery of novel and effective therapies for this disease depends on reliable animal models. Here, we describe a method to generate syngeneic PCa mouse models based on intraperitoneal (i.p.) administration of tumor cells. This model allows to follow-up cancer progression in PCa models from ovarian and colorectal origins monitoring mice bodyweight changes, ascites development and overall survival. Moreover, luciferase-expressing tumor cells can also be used to assess tumor growth after i.p. injection through in vivo bioluminescence quantification. The establishment of reliable, easy-to-monitor and reproducible intraperitoneal syngeneic tumors models, as described here, is the first step to develop cutting-edge therapies against PCa. Competing Interests: Disclosures This study was supported by Instituto de Salud Carlos III (PI19/01128, and PI20/00002) co-financed by Fondos Feder and Gobierno de Navarra Proyecto ARNMUNE Ref: 0011-1411-2023. P.B. receives a 2022 Leonardo Grant for Researchers and Cultural Creators (BBVA Foundation). F.A. receives a Miguel Servet I (CP19/00114) contract from ISCIII (Instituto de Salud Carlos III) co-financed by FSE (Fondo Social Europeo). A.B. is the recipient of PFIS fellowship from ISCIII (FI20/00058), and L.A. receives a FPU grant (FPU21/00042) from Spanish Ministry of Education and Professional training. (Copyright © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.) |
| فهرسة مساهمة: | Keywords: Animal models; Ascites; Omentum; Peritoneal carcinomatosis; Tumor models |
| تواريخ الأحداث: | Date Created: 20240331 Date Completed: 20240402 Latest Revision: 20240402 |
| رمز التحديث: | 20240402 |
| DOI: | 10.1016/bs.mcb.2024.02.005 |
| PMID: | 38556452 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0091-679X |
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| DOI: | 10.1016/bs.mcb.2024.02.005 |