Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV.

التفاصيل البيبلوغرافية
العنوان: Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV.
المؤلفون: Chao CW; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Graduate Program in Molecular and Cellular Biology, University of Washington, Seattle, WA 98195, USA., Sprouse KR; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Miranda MC; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Catanzaro NJ; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Hubbard ML; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Addetia A; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Stewart C; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Brown JT; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Dosey A; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Valdez A; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Ravichandran R; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Hendricks GG; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Ahlrichs M; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Dobbins C; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Hand A; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Treichel C; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Willoughby I; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Walls AC; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., McGuire AT; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Leaf EM; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Baric RS; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Schäfer A; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Veesler D; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Howard Hughes Medical Institute, Seattle, WA 98195, USA., King NP; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
المصدر: BioRxiv : the preprint server for biology [bioRxiv] 2024 Mar 14. Date of Electronic Publication: 2024 Mar 14.
نوع المنشور: Preprint
اللغة: English
بيانات الدورية: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE
مستخلص: Middle East respiratory syndrome coronavirus (MERS-CoV) is a zoonotic betacoronavirus that causes severe and often lethal respiratory illness in humans. The MERS-CoV spike (S) protein is the viral fusogen and the target of neutralizing antibodies, and has therefore been the focus of vaccine design efforts. Currently there are no licensed vaccines against MERS-CoV and only a few candidates have advanced to Phase I clinical trials. Here we developed MERS-CoV vaccines utilizing a computationally designed protein nanoparticle platform that has generated safe and immunogenic vaccines against various enveloped viruses, including a licensed vaccine for SARS-CoV-2. Two-component protein nanoparticles displaying MERS-CoV S-derived antigens induced robust neutralizing antibody responses and protected mice against challenge with mouse-adapted MERS-CoV. Electron microscopy polyclonal epitope mapping and serum competition assays revealed the specificities of the dominant antibody responses elicited by immunogens displaying the prefusion-stabilized S-2P trimer, receptor binding domain (RBD), or N-terminal domain (NTD). An RBD nanoparticle vaccine elicited antibodies targeting multiple non-overlapping epitopes in the RBD, whereas anti-NTD antibodies elicited by the S-2P- and NTD-based immunogens converged on a single antigenic site. Our findings demonstrate the potential of two-component nanoparticle vaccine candidates for MERS-CoV and suggest that this platform technology could be broadly applicable to betacoronavirus vaccine development.
References: Science. 2021 Dec 24;374(6575):1621-1626. (PMID: 34751595)
Nat Commun. 2021 Mar 17;12(1):1715. (PMID: 33731724)
Protein Sci. 2018 Jan;27(1):14-25. (PMID: 28710774)
Cell. 2021 Apr 29;184(9):2332-2347.e16. (PMID: 33761326)
Lancet Infect Dis. 2020 Jul;20(7):816-826. (PMID: 32325038)
Nature. 2020 Aug;584(7821):437-442. (PMID: 32555388)
Nature. 2020 Mar;579(7798):270-273. (PMID: 32015507)
Science. 2022 Aug 12;377(6607):735-742. (PMID: 35857703)
Science. 2020 Mar 13;367(6483):1260-1263. (PMID: 32075877)
Nat Struct Mol Biol. 2019 Dec;26(12):1151-1157. (PMID: 31792450)
Proc Natl Acad Sci U S A. 2021 Sep 21;118(38):. (PMID: 34493582)
Immunity. 2005 Aug;23(2):165-75. (PMID: 16111635)
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7348-E7357. (PMID: 28807998)
EClinicalMedicine. 2023 Sep 07;64:102140. (PMID: 37711219)
Virology. 2018 Apr;517:98-107. (PMID: 29277291)
Science. 2022 Aug 5;377(6606):eabq0839. (PMID: 35857620)
J Virol. 2018 Apr 27;92(10):. (PMID: 29514901)
Science. 2021 Aug 6;373(6555):648-654. (PMID: 34210893)
Cell Rep. 2022 Jan 11;38(2):110217. (PMID: 35021101)
iScience. 2022 Dec 22;25(12):105649. (PMID: 36439375)
Cell. 2020 Nov 25;183(5):1367-1382.e17. (PMID: 33160446)
Cell. 2020 Dec 10;183(6):1732. (PMID: 33306956)
Cell Rep. 2023 Oct 31;42(10):113248. (PMID: 37858337)
Nat Commun. 2023 Oct 4;14(1):6195. (PMID: 37794071)
Emerg Microbes Infect. 2019;8(1):516-530. (PMID: 30938227)
Cell. 2021 Aug 19;184(17):4593-4595. (PMID: 34416148)
Cell. 2021 Mar 4;184(5):1188-1200.e19. (PMID: 33577765)
J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254)
Cell Res. 2015 Nov;25(11):1237-49. (PMID: 26391698)
Nature. 2013 Aug 8;500(7461):227-31. (PMID: 23831647)
Proc Natl Acad Sci U S A. 2021 Sep 21;118(38):. (PMID: 34470866)
NPJ Vaccines. 2022 May 23;7(1):55. (PMID: 35606518)
Nature. 2020 Oct;586(7830):567-571. (PMID: 32756549)
Proc Natl Acad Sci U S A. 2015 Aug 18;112(33):10473-8. (PMID: 26216974)
Sci Immunol. 2020 Jun 11;5(48):. (PMID: 32527802)
Nat Methods. 2017 Mar;14(3):290-296. (PMID: 28165473)
EClinicalMedicine. 2021 Nov;41:101191. (PMID: 34805807)
Cell. 2021 Apr 1;184(7):1804-1820.e16. (PMID: 33691139)
Nature. 2023 Sep;621(7979):592-601. (PMID: 37648855)
Sci Transl Med. 2014 Apr 30;6(234):234ra59. (PMID: 24778414)
Science. 2021 Feb 12;371(6530):735-741. (PMID: 33436524)
Nat Commun. 2022 Oct 23;13(1):6309. (PMID: 36274085)
Cell Rep. 2019 Sep 24;28(13):3395-3405.e6. (PMID: 31553909)
PLoS Pathog. 2020 Aug 11;16(8):e1008665. (PMID: 32780770)
Adv Sci (Weinh). 2024 Mar;11(9):e2303366. (PMID: 38105421)
Nature. 2020 Aug;584(7821):450-456. (PMID: 32698192)
Science. 2016 Jul 22;353(6297):389-94. (PMID: 27463675)
Proc Natl Acad Sci U S A. 2017 Oct 3;114(40):E8508-E8517. (PMID: 28923942)
Sci Transl Med. 2022 Aug 17;14(658):eabq4130. (PMID: 35976993)
Nat Commun. 2015 Jul 28;6:7712. (PMID: 26218507)
Emerg Microbes Infect. 2020 Dec;9(1):1080-1091. (PMID: 32471334)
Nature. 2021 Jun;594(7862):253-258. (PMID: 33873199)
Nat Commun. 2021 Jan 12;12(1):324. (PMID: 33436573)
NPJ Vaccines. 2021 May 13;6(1):70. (PMID: 33986287)
Cell. 2020 Dec 10;183(6):1735. (PMID: 33306958)
Nat Biomed Eng. 2020 Dec;4(12):1128-1133. (PMID: 33293724)
Cell Host Microbe. 2021 May 12;29(5):819-833.e7. (PMID: 33789084)
Science. 2020 Sep 18;369(6510):1501-1505. (PMID: 32703906)
Cell Rep. 2021 Dec 21;37(12):110143. (PMID: 34919799)
Nature. 2021 Sep;597(7874):97-102. (PMID: 34261126)
Nat Commun. 2019 Jul 11;10(1):3068. (PMID: 31296843)
Proc Natl Acad Sci U S A. 2014 May 13;111(19):E2018-26. (PMID: 24778221)
Nat Commun. 2021 Feb 9;12(1):883. (PMID: 33563988)
NPJ Vaccines. 2020 Aug 5;5(1):72. (PMID: 32802411)
PLoS Pathog. 2020 Dec 28;16(12):e1009169. (PMID: 33370407)
One Health Outlook. 2022 Jun 24;4(1):12. (PMID: 35739576)
Nat Commun. 2022 May 13;13(1):2674. (PMID: 35562337)
Cell. 2019 Mar 7;176(6):1420-1431.e17. (PMID: 30849373)
Nat Commun. 2023 Apr 17;14(1):2149. (PMID: 37069151)
Cell Host Microbe. 2020 Sep 9;28(3):445-454.e6. (PMID: 32585135)
Commun Biol. 2022 Apr 11;5(1):342. (PMID: 35411021)
Cell Host Microbe. 2022 Dec 14;30(12):1759-1772.e12. (PMID: 36400021)
Nature. 2013 Mar 14;495(7440):251-4. (PMID: 23486063)
Science. 2021 Sep 03;373(6559):1109-1116. (PMID: 34344823)
Nat Commun. 2019 Sep 19;10(1):4272. (PMID: 31537780)
Sci Transl Med. 2023 Sep 27;15(715):eadg5567. (PMID: 37756379)
Nature. 2021 Jun;594(7864):553-559. (PMID: 33971664)
Nat Microbiol. 2016 Nov 28;2:16226. (PMID: 27892925)
Elife. 2023 Mar 21;12:. (PMID: 36942851)
Vaccine. 2014 May 30;32(26):3169-3174. (PMID: 24736006)
Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):3048-53. (PMID: 26976607)
Sci Adv. 2022 May 6;8(18):eabn2911. (PMID: 35507649)
J Infect Dis. 2023 Jun 15;227(12):1433-1441. (PMID: 35876700)
Science. 2020 Nov 27;370(6520):1089-1094. (PMID: 33082295)
Elife. 2020 Aug 04;9:. (PMID: 32748788)
Lancet Infect Dis. 2020 Jul;20(7):827-838. (PMID: 32325037)
Immunity. 2023 Mar 14;56(3):669-686.e7. (PMID: 36889306)
Emerg Microbes Infect. 2017 Jun 28;6(6):e60. (PMID: 28655936)
Nat Struct Mol Biol. 2021 Jun;28(6):478-486. (PMID: 33981021)
Cell. 2018 Mar 8;172(6):1319-1334. (PMID: 29522750)
Front Microbiol. 2022 Sep 28;13:988298. (PMID: 36246239)
Nat Commun. 2022 Nov 25;13(1):7271. (PMID: 36434005)
Sci Transl Med. 2021 Jun 30;13(600):. (PMID: 34103407)
J Exp Med. 2021 Mar 1;218(3):. (PMID: 33211088)
ACS Nano. 2021 Feb 23;15(2):2738-2752. (PMID: 33464829)
Cell Res. 2013 Aug;23(8):986-93. (PMID: 23835475)
Cell Rep. 2021 Nov 2;37(5):109929. (PMID: 34710354)
Ann Intern Med. 2014 Mar 18;160(6):389-97. (PMID: 24474051)
Sci Immunol. 2022 Dec 23;7(78):eadf1421. (PMID: 36356052)
Vaccine. 2023 Aug 7;41(35):5201-5210. (PMID: 37451877)
EClinicalMedicine. 2022 Jul 22;51:101569. (PMID: 35879941)
Nat Commun. 2021 Jan 22;12(1):542. (PMID: 33483491)
Front Immunol. 2021 Jun 29;12:710263. (PMID: 34267764)
Nature. 2021 Apr;592(7855):623-628. (PMID: 33762730)
Immunity. 2023 Oct 10;56(10):2425-2441.e14. (PMID: 37689061)
Lancet Infect Dis. 2019 Sep;19(9):1013-1022. (PMID: 31351922)
NPJ Vaccines. 2022 Feb 28;7(1):27. (PMID: 35228534)
Nat Commun. 2024 Jan 3;15(1):200. (PMID: 38172512)
Nat Med. 2015 Dec;21(12):1508-13. (PMID: 26552008)
Nature. 2020 Dec;588(7839):682-687. (PMID: 33045718)
Immunity. 2022 Dec 13;55(12):2419-2435.e10. (PMID: 36370711)
Nat Microbiol. 2022 Jul;7(7):1063-1074. (PMID: 35773398)
Cell. 2021 Oct 14;184(21):5432-5447.e16. (PMID: 34619077)
Immunity. 2020 Jul 14;53(1):98-105.e5. (PMID: 32561270)
Nat Commun. 2017 Nov 23;8(1):1735. (PMID: 29170370)
Sci Transl Med. 2023 May 10;15(695):eadg7404. (PMID: 37163615)
Nat Microbiol. 2023 Apr;8(4):569-580. (PMID: 37012355)
Cell. 2020 Nov 12;183(4):1024-1042.e21. (PMID: 32991844)
Science. 2022 Feb 25;375(6583):864-868. (PMID: 35076256)
Science. 2021 Mar 25;:. (PMID: 33766944)
Immunity. 2018 Aug 21;49(2):288-300.e8. (PMID: 30097292)
Nat Struct Mol Biol. 2016 Sep;23(9):811-820. (PMID: 27478931)
معلومات مُعتمدة: 75N93022C00036 United States AI NIAID NIH HHS; P01 AI167966 United States AI NIAID NIH HHS
تواريخ الأحداث: Date Created: 20240401 Latest Revision: 20240405
رمز التحديث: 20240405
مُعرف محوري في PubMed: PMC10979991
DOI: 10.1101/2024.03.13.584735
PMID: 38558973
قاعدة البيانات: MEDLINE
الوصف
DOI:10.1101/2024.03.13.584735