Contrast-enhanced ultrasound imaging detects anatomical and functional changes in rat cervical spine microvasculature with normal aging.
| العنوان: | Contrast-enhanced ultrasound imaging detects anatomical and functional changes in rat cervical spine microvasculature with normal aging. |
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| المؤلفون: | Harmon JN; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA., Chandran P; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA., Chandrasekaran A; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA., Hyde JE; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA., Hernandez GJ; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA., Reed MJ; Department of Gerontology and Geriatric Medicine, University of Washington, Seattle, WA, USA., Bruce MF; Applied Physics Laboratory, University of Washington, Seattle, WA, USA., Khaing ZZ; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Mar 14. Date of Electronic Publication: 2024 Mar 14. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet ISSN: 2692-8205 (Electronic) Linking ISSN: 26928205 NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | Normal aging is associated with significant deleterious cerebrovascular changes; these have been implicated in disease pathogenesis and increased susceptibility to ischemic injury. While these changes are well documented in the brain, few studies have been conducted in the spinal cord. Here, we utilize specialized contrast-enhanced ultrasound (CEUS) imaging to investigate age-related changes in cervical spinal vascular anatomy and hemodynamics in male Fisher 344 rats, a common strain in aging research. Aged rats (24-26 mo., N=6) exhibited significant tortuosity in the anterior spinal artery and elevated vascular resistance compared to adults (4-6 mo., N=6; tortuosity index 2.20±0.15 vs 4.74±0.45, p<0.05). Baseline blood volume was lower in both larger vessels and the microcirculation in the aged cohort, specifically in white matter (4.44e14±1.37e13 vs 3.66e14±2.64e13 CEUS bolus AUC, p<0.05). To elucidate functional differences, animals were exposed to a hypoxia challenge; whereas adult rats exhibited significant functional hyperemia in both gray and white matter (GM: 1.13±0.10-fold change from normoxia, p<0.05; WM: 1.16±0.13, p<0.05), aged rats showed no response. Immunohistochemistry revealed reduced pericyte coverage and activated microglia behavior in aged rats, which may partially explain the lack of vascular response. This study provides the first in vivo description of age-related hemodynamic differences in the cervical spinal cord. Competing Interests: Competing interests Declarations of interest: none. |
| التعليقات: | Update in: J Gerontol A Biol Sci Med Sci. 2024 Aug 27:glae215. doi: 10.1093/gerona/glae215. (PMID: 39188137) |
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| معلومات مُعتمدة: | F32 HD107806 United States HD NICHD NIH HHS; R01 NS121191 United States NS NINDS NIH HHS; R03 AG073929 United States AG NIA NIH HHS; R21 AG073676 United States AG NIA NIH HHS |
| فهرسة مساهمة: | Keywords: Cervical spinal cord; hypoxia; perfusion; pericytes |
| تواريخ الأحداث: | Date Created: 20240401 Latest Revision: 20240903 |
| رمز التحديث: | 20240903 |
| مُعرف محوري في PubMed: | PMC10980054 |
| DOI: | 10.1101/2024.03.12.584672 |
| PMID: | 38559128 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2692-8205 |
|---|---|
| DOI: | 10.1101/2024.03.12.584672 |