Age-related Morphofunctional Changes in Sickle Cell Mice Bone Marrow Mesenchymal Stromal Cells.

التفاصيل البيبلوغرافية
العنوان:	Age-related Morphofunctional Changes in Sickle Cell Mice Bone Marrow Mesenchymal Stromal Cells.
المؤلفون:	Rós FA; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil.; Laboratory of Medical Investigation in Pathogenesis and Directed Therapy in Onco-Immuno-Hematology (LIM-31), Department of Hematology and Cell Therapy, Hospital das Clínicas da Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo, 01246-000, Brazil., da Costa PNM; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil., Milhomens J; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil., de La-Roque DGL; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil., Ferreira FU; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil., de Matos Maçonetto J; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil., de Oliveira Menezes Bonaldo CC; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil., de Carvalho JV; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil., Palma PVB; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil., El Nemer W; Établissement Français du Sang PACA-Corse, Aix Marseille University, EFS, CNRS, ADES, 'Biologie des Groupes Sanguins', F-13005, Marseille, France., Covas DT; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil., Kashima S; Blood Center of Ribeirão Preto - Ribeirão Preto Medical School, University of São Paulo, 2501 Tenente Catão Roxo Avenue, 14051-060, Ribeirão Preto, São Paulo, Brazil.
المصدر:	Hematology/oncology and stem cell therapy [Hematol Oncol Stem Cell Ther] 2024 Mar 22; Vol. 17 (2), pp. 120-129.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: King Faisal Cancer Center, King Faisal Specialist Hospital and Research Centre Country of Publication: Saudi Arabia NLM ID: 101468532 Publication Model: Print Cited Medium: Internet ISSN: 2589-0646 (Electronic) Linking ISSN: 25890646 NLM ISO Abbreviation: Hematol Oncol Stem Cell Ther Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Riyadh : King Faisal Cancer Center, King Faisal Specialist Hospital and Research Centre
مواضيع طبية MeSH:	Mesenchymal Stem Cells/metabolism , Anemia, Sickle Cell, Humans ; Animals ; Mice ; Bone Marrow ; Mice, Inbred C57BL ; Hematopoietic Stem Cells/metabolism ; Bone Marrow Cells/metabolism ; Cell Differentiation
مستخلص:	Background and Objectives: Bone marrow mesenchymal stromal cells (BM-MSCs) are key elements of the hematopoietic niche and participate in the regulatory mechanisms of hematopoietic stem cells (HSCs). Hematological diseases can affect MSCs and their functions. However, the dysregulations caused by sickle cell disease (SCD) are not fully elucidated. This work explored changes in BM-MSCs and their relationship with age using sickle cell mice (Townes-SS). Materials and Methods: BM-MSCs were isolated from Townes-SS, and control groups 30- and 60-day-old Townes-AA and C57BL/6 J. Results: The BM-MSCs showed no morphological differences in culture and demonstrated a murine MSC-like immunophenotypic profile (Sca-1+, CD29+, CD44+, CD90.2+, CD31-, CD45-, and CD117-). Subsequently, all BM-MSCs were able to differentiate into adipocytes and osteocytes in vitro. Finally, 30-day-old BM-MSCs of Townes-SS showed higher expression of genes related to the maintenance of HSCs (Cxcl12, Vegfa, and Angpt1) and lower expression of pro-inflammatory genes (Tnfa and Il-6). However, 60-day-old BM-MSCs of Townes-SS started to show expression of genes related to reduced HSC maintenance and increased expression of pro-inflammatory genes. Conclusion: These results indicates age as a modifying factor of gene expression of BM-MSCs in the context of SCD.
تواريخ الأحداث:	Date Created: 20240401 Date Completed: 20240403 Latest Revision: 20240403
رمز التحديث:	20240403
DOI:	10.56875/2589-0646.1115
PMID:	38560971
قاعدة البيانات:	MEDLINE

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تدمد:	2589-0646
DOI:	10.56875/2589-0646.1115