دورية أكاديمية
The Use of the Internal Transcribed Spacer Region for Phylogenetic Analysis of the Microsporidian Parasite Enterocytozoon hepatopenaei Infecting Whiteleg Shrimp ( Penaeus vannamei ) and for the Development of a Nested PCR as Its Diagnostic Tool.
| العنوان: | The Use of the Internal Transcribed Spacer Region for Phylogenetic Analysis of the Microsporidian Parasite Enterocytozoon hepatopenaei Infecting Whiteleg Shrimp ( Penaeus vannamei ) and for the Development of a Nested PCR as Its Diagnostic Tool. |
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| المؤلفون: | Lee JH; Laboratory of Aquatic Biomedicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea., Jeon HJ; Laboratory of Aquatic Biomedicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea., Seo S; Laboratory of Aquatic Biomedicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea., Lee C; Laboratory of Aquatic Biomedicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea., Kim B; Laboratory of Aquatic Biomedicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea., Kwak DM; Laboratory of Aquatic Biomedicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea., Rhee MH; Laboratory of Aquatic Biomedicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea., Piamsomboon P; Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.; Veterinary Medical Aquatic Animal Research Center of Excellence, Chulalongkorn University, Bangkok, Thailand., Nuraini YL; Fish Health and Environmental Laboratory, Brackishwater Aquaculture Development Center, Situbondo, Indonesia., Je CU; Ministry of Agriculture, Food and Rural Affairs, Sejong 30110, Republic of Korea., Park SY; Division of Animal and Dairy Sciences, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Republic of Korea., Kim JH; Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of Korea., Han JE; Laboratory of Aquatic Biomedicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.; Institute for Veterinary Biomedical Science, Kyungpook National University, Daegu 41566, Republic of Korea. |
| المصدر: | Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2024 May 28; Vol. 34 (5), pp. 1146-1153. Date of Electronic Publication: 2024 Feb 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Korean Society for Microbiology and Biotechnology Country of Publication: Korea (South) NLM ID: 9431852 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1738-8872 (Electronic) Linking ISSN: 10177825 NLM ISO Abbreviation: J Microbiol Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2002- : Seoul : Korean Society for Microbiology and Biotechnology Original Publication: Seoul : Korean Society for Applied Microbiology, 1991-2001. |
| مواضيع طبية MeSH: | Enterocytozoon*/genetics , Enterocytozoon*/isolation & purification , Enterocytozoon*/classification , Penaeidae*/microbiology , Penaeidae*/parasitology , Phylogeny* , DNA, Ribosomal Spacer*/genetics , Polymerase Chain Reaction*/methods , Microsporidiosis*/microbiology , Microsporidiosis*/diagnosis, Animals ; DNA, Fungal/genetics ; DNA Primers/genetics ; Feces/microbiology ; Feces/parasitology ; Sequence Analysis, DNA ; Thailand |
| مستخلص: | The increasing economic losses associated with growth retardation caused by Enterocytozoon hepatopenaei (EHP), a microsporidian parasite infecting penaeid shrimp, require effective monitoring. The internal transcribed spacer (ITS)-1 region, the non-coding region of ribosomal clusters between 18S and 5.8S rRNA genes, is widely used in phylogenetic studies due to its high variability. In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp ( Penaeus vannamei and P. monodon ) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) genes. Phylogenetic analysis based on the ITS-1 sequences indicated that the Indonesian strain was distinct (86.2% nucleotide sequence identity) from other strains collected from Thailand and South Korea, and also showed the internal diversity among Thailand ( N = 7, divided into four branches) and South Korean ( N = 5, divided into two branches) samples. The results revealed the ability of the ITS-1 region to determine the genetic diversity of EHP from different geographical origins. |
| References: | Bioinformatics. 2007 Nov 1;23(21):2947-8. (PMID: 17846036) Adv Parasitol. 2014;84:209-52. (PMID: 24480315) Trends Genet. 2015 Mar;31(3):157-63. (PMID: 25648500) Mar Genomics. 2017 Dec;36:33-39. (PMID: 28578827) Front Cell Infect Microbiol. 2021 Feb 25;11:631960. (PMID: 33718281) PLoS One. 2016 Nov 10;11(11):e0166320. (PMID: 27832178) Parasitol Res. 2018 May;117(5):1485-1493. (PMID: 29550998) PLoS One. 2012;7(9):e43117. (PMID: 23028445) Nat Commun. 2019 Apr 12;10(1):1702. (PMID: 30979905) Environ Microbiol. 2017 May;19(5):2077-2089. (PMID: 28345194) Dis Aquat Organ. 2016 Aug 9;120(3):225-30. (PMID: 27503918) Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. (PMID: 29722887) Pathogens. 2019 Nov 12;8(4):. (PMID: 31726681) J Invertebr Pathol. 2009 Sep;102(1):21-9. (PMID: 19527727) Infect Genet Evol. 2015 Dec;36:244-249. (PMID: 26423670) J Invertebr Pathol. 2015 Sep;130:37-41. (PMID: 26146228) Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6241-6. (PMID: 22454494) J Comput Biol. 2012 May;19(5):455-77. (PMID: 22506599) Mar Biotechnol (NY). 2001 Jul;3(4):355-61. (PMID: 14961351) Appl Environ Microbiol. 2010 May;76(10):3333-42. (PMID: 20228101) Mol Biochem Parasitol. 1992 Dec;56(2):323-7. (PMID: 1484553) Dis Aquat Organ. 2004 Aug 9;60(2):89-96. (PMID: 15460852) |
| فهرسة مساهمة: | Keywords: Enterocytozoon hepatopenaei; internal transcribed spacer; microsporidia; phylogeny; polymerase chain reaction; shrimp |
| المشرفين على المادة: | 0 (DNA, Ribosomal Spacer) 0 (DNA, Fungal) 0 (DNA Primers) |
| SCR Organism: | Enterocytozoon hepatopenaei |
| تواريخ الأحداث: | Date Created: 20240402 Date Completed: 20240528 Latest Revision: 20240619 |
| رمز التحديث: | 20240619 |
| مُعرف محوري في PubMed: | PMC11180916 |
| DOI: | 10.4014/jmb.2401.01010 |
| PMID: | 38563108 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1738-8872 |
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| DOI: | 10.4014/jmb.2401.01010 |