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In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β-galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450.

التفاصيل البيبلوغرافية
العنوان: In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β-galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450.
المؤلفون: Fara A; Laboratorio de Ecofisiología Tecnológica, CERELA-CONICET, San Miguel de Tucumán, Argentina., Hernández Hernández O; Grupo de Química y Funcionalidad de Carbohidratos y Derivados, Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CSIC-UAM), Madrid, Spain., Palacios J; Laboratorio de Ecofisiología Tecnológica, CERELA-CONICET, San Miguel de Tucumán, Argentina., Montilla A; Grupo de Química y Funcionalidad de Carbohidratos y Derivados, Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CSIC-UAM), Madrid, Spain., Zárate G; Laboratorio de Ecofisiología Tecnológica, CERELA-CONICET, San Miguel de Tucumán, Argentina.; Universidad de San Pablo Tucumán, Av. Solano Vera y Camino a Villa Nougués, Tucumán, Argentina.
المصدر: Journal of the science of food and agriculture [J Sci Food Agric] 2024 Aug 30; Vol. 104 (11), pp. 6769-6777. Date of Electronic Publication: 2024 Apr 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons Country of Publication: England NLM ID: 0376334 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0010 (Electronic) Linking ISSN: 00225142 NLM ISO Abbreviation: J Sci Food Agric Subsets: MEDLINE
أسماء مطبوعة: Publication: <2005-> : Chichester, West Sussex : John Wiley & Sons
Original Publication: London, Society of Chemical Industry.
مواضيع طبية MeSH: Prebiotics*/analysis , beta-Galactosidase*/metabolism , beta-Galactosidase*/chemistry , Oligosaccharides*/metabolism , Oligosaccharides*/chemistry , Digestion* , Mice, Inbred C57BL*, Animals ; Mice ; Rats ; Swine ; Male ; Lactulose/metabolism ; Lactulose/chemistry ; Bacterial Proteins/metabolism ; Bacterial Proteins/chemistry ; Intestine, Small/metabolism ; Intestine, Small/enzymology ; Lactobacillus/metabolism ; Lactobacillus/enzymology ; Hydrolysis ; Lactose/metabolism ; Lactose/chemistry
مستخلص: Background: The general assumption that prebiotics reach the colon without any alterations has been challenged. Some in vitro and in vivo studies have demonstrated that 'non-digestible' oligosaccharides are digested to different degrees depending on their structural composition. In the present study, we compared different methods aiming to assess the digestibility of oligosaccharides synthesized by β-galactosidase (β-gal) of Lactobacillus delbruecki subsp. bulgaricus CRL450 (CRL450-β-gal) from lactose, lactulose and lactitol.
Results: In the simulated gastrointestinal fluid method, no changes were observed. However, the oligosaccharides synthesized by CRL450-β-gal were partially hydrolyzed in vitro, depending on their structure and composition, with rat small intestinal extract (RSIE) and small intestinal brush-border membrane vesicles (BBMV) from pig. Digestion of some oligosaccharides increased when mixtures were fed to C57BL/6 mice used as in vivo model; however, lactulose-oligosaccharides were the most resistant to the physiological conditions of mice. In general β (1→6) linked products showed higher resistance compared to β (1→3) oligosaccharides.
Conclusion: In vitro digestion methods, without disaccharidases, may underestimate the importance of carbohydrates hydrolysis in the small intestine. Although BVMM and RSIE digestion assays are appropriate in vitro methods for these studies, in vivo studies remain the most reliable for understanding what actually happens in the digestion of oligosaccharides. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
(© 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.)
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معلومات مُعتمدة: European Regional Development Fund; Agencia Estatal de Investigación; Consejo Superior de Investigaciones Científicas; Ministerio de Ciencia e Innovación; Universidad de San Pablo Tucumán, Argentina; Agencia Nacional de Promoción Científica y Tecnológica, Argentina.
فهرسة مساهمة: Keywords: Lactobacillus delbruecki subsp. bulgaricus; oligosaccharides; brush border membrane vesicles (BBMV); in vivo digestion; rat small intestinal extract (RSIE)
المشرفين على المادة: 0 (Prebiotics)
EC 3.2.1.23 (beta-Galactosidase)
0 (Oligosaccharides)
4618-18-2 (Lactulose)
0 (Bacterial Proteins)
J2B2A4N98G (Lactose)
تواريخ الأحداث: Date Created: 20240402 Date Completed: 20240713 Latest Revision: 20240718
رمز التحديث: 20240718
DOI: 10.1002/jsfa.13504
PMID: 38563403
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-0010
DOI:10.1002/jsfa.13504