دورية أكاديمية
أعرض في EDS

Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineus.

التفاصيل البيبلوغرافية
العنوان: Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineus.
المؤلفون: Zhao X; Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan University, Haikou, 570228, Hainan, China.; One Health Institute, Hainan University, Haikou, 570228, Hainan, China., Zhao J; Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan University, Haikou, 570228, Hainan, China.; One Health Institute, Hainan University, Haikou, 570228, Hainan, China., Wang J; Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan University, Haikou, 570228, Hainan, China.; One Health Institute, Hainan University, Haikou, 570228, Hainan, China., Liao C; Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan University, Haikou, 570228, Hainan, China.; One Health Institute, Hainan University, Haikou, 570228, Hainan, China., Guan Q; Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan University, Haikou, 570228, Hainan, China. 992930@hainanu.edu.cn.; One Health Institute, Hainan University, Haikou, 570228, Hainan, China. 992930@hainanu.edu.cn., Han Q; Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life Sciences, Hainan University, Haikou, 570228, Hainan, China. qianhan@hainanu.edu.cn.; One Health Institute, Hainan University, Haikou, 570228, Hainan, China. qianhan@hainanu.edu.cn.
المصدر: Scientific reports [Sci Rep] 2024 Apr 02; Vol. 14 (1), pp. 7703. Date of Electronic Publication: 2024 Apr 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Rhipicephalus sanguineus* , Rhipicephalus*/metabolism , Vaccines* , Animals, Outbred Strains*, Mice ; Animals ; Serine Proteinase Inhibitors/metabolism ; Nymph ; Larva
مستخلص: Bioactive molecules in tick saliva are considered to be key to successful feeding and further the transmission of tick-borne pathogens. Problems such as pathogen transmission and animal weight loss result in tick infestation can cause tremendous economic losses to the livestock industry. Therefore, the development of a universal tick vaccine is urgently needed. In this paper, three serine protease inhibitor (serpin) proteins RMS-3, L7LRK7 and L7LTU1 were analyzed with bioinformatics methods. Subsequently the proteins were expressed and purified, and inoculated into Kunming mice for immune protection analysis. The amino acid sequence similarities between RMS-3, L7LRK7 and L7LTU1 were up to 90% in Rhipicephalus sanguineus. The recombinant RMS-3 + L7LRK7 + L7LTU1 showed anticoagulant reaction function and could inhibit the activity of CD4 + lymphocytes, when inoculated into Kunming mice. Additionally, After the immunized mice were challenged with Rhipicephalus sanguineus, the percentage of larvae and nymphs that were fully engorged dropped to 40.87% (P < 0.05) and 87.68% (P > 0.05) in the RmS-3 + L7LRK7 immune group, 49.57% (P < 0.01) and 52.06% (P < 0.05) in the RmS-3 + L7LTU1 group, and 45.22% (P < 0.05) and 60.28% (P < 0.05) in the RmS-3 + L7LRK7 + L7LTU1 immune group, in comparison with the control group. These data indicate that RmS-3 + L7LRK7 + L7LTU1 has good immune protection and has the potential to be developed into a vaccine against the larvae and nymphs of R. sanguineus.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 821RC530 Natural Science Foundation of Hainan Province; ZDKJ2021035 Hainan Province Science and Technology Special Fund; U22A20363 National Natural Science Foundation of China
فهرسة مساهمة: Keywords: R. sanguineus; Bioinformatics; Immune protection; Serpins; Vaccine
المشرفين على المادة: 0 (Serine Proteinase Inhibitors)
0 (Vaccines)
SCR Organism: Kunming mice
تواريخ الأحداث: Date Created: 20240402 Date Completed: 20240404 Latest Revision: 20240405
رمز التحديث: 20240405
مُعرف محوري في PubMed: PMC10987660
DOI: 10.1038/s41598-024-58303-4
PMID: 38565937
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-58303-4