دورية أكاديمية
Differences in diversity and community composition of the shell microbiome of apparently healthy lobsters Homarus americanus across Atlantic Canada.
العنوان: | Differences in diversity and community composition of the shell microbiome of apparently healthy lobsters Homarus americanus across Atlantic Canada. |
---|---|
المؤلفون: | Koepper S; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada., Clark KF; Department of Animal Sciences and Aquaculture, Faculty of Agriculture, Dalhousie University, Truro, NS, Canada., McClure JT; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada., Revie CW; Department of Computer and Information Sciences, University of Strathclyde, Glasgow, United Kingdom., Stryhn H; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada., Thakur KK; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada. |
المصدر: | Frontiers in microbiology [Front Microbiol] 2024 Mar 18; Vol. 15, pp. 1320812. Date of Electronic Publication: 2024 Mar 18 (Print Publication: 2024). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
مستخلص: | Host-microbe dynamics are of increasing interest in marine research due to their role in host health and productivity. Changes in the shell microbiome of American lobsters have been associated with epizootic shell disease, a syndrome that is spreading northwards across the eastern U.S. and Canadian Atlantic coast. This study analyzed differences in alpha and beta diversity, as well as differentially abundant taxa, in the shell-associated bacterial community of apparently healthy lobsters from four lobster fishing areas (LFAs) in Atlantic Canada. Over 180 lobsters from New Brunswick, Nova Scotia and Prince Edward Island (PEI) were sampled during seven sampling events over four sampling months. The bacterial community was identified using novel PacBio long-read sequencing, while alpha and beta diversity parameters were analyzed using linear regression models and weighted UniFrac distances. The bacterial richness, diversity and evenness differed by sampling location, sampling month, and molt stage, but not by lobster sex or size, nor sampling depth. Similarly, based on LFA, sampling month, year and lobster molt stage, the shell microbiome differed in microbial community composition with up to 34 out of 162 taxa differing significantly in abundance between sampling groups. This large-scale microbial survey suggests that the shell microbial diversity of apparently healthy lobsters is influenced by spatial and temporal factors such as geographic location, as well as the length of time the carapace is exposed to the surrounding seawater. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Koepper, Clark, McClure, Revie, Stryhn and Thakur.) |
References: | Nat Commun. 2022 Jan 17;13(1):342. (PMID: 35039521) Cell. 2011 Sep 30;147(1):44-56. (PMID: 21962506) Front Microbiol. 2023 Apr 05;14:1093312. (PMID: 37089549) Arch Microbiol. 2021 Sep;203(7):3793-3802. (PMID: 34120200) Microb Ecol Health Dis. 2015 May 29;26:27663. (PMID: 26028277) Front Microbiol. 2023 Nov 06;14:1245818. (PMID: 38029079) J Hazard Mater. 2022 Jul 5;433:128832. (PMID: 35390615) Curr Psychiatry Rep. 2013 Feb;15(2):337. (PMID: 23307560) Sci Rep. 2021 Dec 16;11(1):24100. (PMID: 34916552) J Exp Bot. 2016 Feb;67(4):995-1002. (PMID: 26547794) Science. 2013 Jul 5;341(6141):1237439. (PMID: 23828941) mSystems. 2018 Mar 13;3(2):. (PMID: 29556539) Environ Microbiol. 2017 Aug;19(8):3087-3097. (PMID: 28464425) Cell Host Microbe. 2012 Nov 15;12(5):611-22. (PMID: 23159051) ISME J. 2017 Jun;11(6):1412-1422. (PMID: 28234350) Front Microbiol. 2017 Nov 07;8:2114. (PMID: 29163406) Comput Struct Biotechnol J. 2015 Jun 09;13:390-401. (PMID: 26137199) ISME J. 2012 Mar;6(3):610-8. (PMID: 22134646) Dis Aquat Organ. 2012 Aug 27;100(2):149-58. (PMID: 23186702) Glob Chang Biol. 2019 Nov;25(11):3906-3917. (PMID: 31344307) PLoS One. 2018 Feb 23;13(2):e0192772. (PMID: 29474389) FEMS Microbiol Ecol. 2019 Jun 1;95(6):. (PMID: 31107952) Microb Ecol. 2008 Apr;55(3):425-34. (PMID: 17624487) J Fish Dis. 2014 Jun;37(6):577-81. (PMID: 23952709) Sci Rep. 2021 Jun 14;11(1):12480. (PMID: 34127690) Front Microbiol. 2016 Jun 21;7:991. (PMID: 27446031) Clin Microbiol Infect. 2012 Jul;18 Suppl 4:2-4. (PMID: 22647038) Sci Rep. 2017 Sep 18;7(1):11783. (PMID: 28924190) Gastroenterology. 2008 Feb;134(2):577-94. (PMID: 18242222) Gigascience. 2019 Sep 1;8(9):. (PMID: 31544212) Am Nat. 2018 Nov;192(5):E163-E177. (PMID: 30332587) Appl Environ Microbiol. 2005 Dec;71(12):8228-35. (PMID: 16332807) NPJ Biofilms Microbiomes. 2020 Dec 2;6(1):60. (PMID: 33268781) Microb Ecol. 2015 Aug;70(2):534-44. (PMID: 25704317) Front Cell Neurosci. 2013 Sep 17;7:153. (PMID: 24062644) Dis Aquat Organ. 2017 Mar 30;124(1):41-54. (PMID: 28357985) Gut Microbes. 2017 May 4;8(3):268-275. (PMID: 28051919) ISME J. 2011 Feb;5(2):169-72. (PMID: 20827291) iScience. 2023 Apr 08;26(5):106606. (PMID: 37128602) Appl Environ Microbiol. 2007 Mar;73(5):1576-85. (PMID: 17220268) mSphere. 2020 May 20;5(3):. (PMID: 32434844) Front Microbiol. 2020 Oct 22;11:586387. (PMID: 33193237) J Anim Ecol. 2010 Nov;79(6):1324-31. (PMID: 20722873) Cell. 2009 Oct 30;139(3):485-98. (PMID: 19836068) Curr Opin Clin Nutr Metab Care. 2010 Jul;13(4):432-8. (PMID: 20531179) |
فهرسة مساهمة: | Keywords: 16S rRNA sequencing; American lobster; UniFrac distance; alpha and beta diversity; aquatic microbiomes |
تواريخ الأحداث: | Date Created: 20240403 Latest Revision: 20240404 |
رمز التحديث: | 20240404 |
مُعرف محوري في PubMed: | PMC10986177 |
DOI: | 10.3389/fmicb.2024.1320812 |
PMID: | 38567078 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1664-302X |
---|---|
DOI: | 10.3389/fmicb.2024.1320812 |