دورية أكاديمية
أعرض في EDS

Establishment of a human ovarian clear cell carcinoma cell line mutant in PIK3CB but not PIK3CA.

التفاصيل البيبلوغرافية
العنوان: Establishment of a human ovarian clear cell carcinoma cell line mutant in PIK3CB but not PIK3CA.
المؤلفون: Hoshino H; Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, 23-3 Matsuoka-Shimoaizuki, Eiheiji, Fukui, 910-1193, Japan., Inoue D; Department of Obstetrics and Gynecology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan., Shinagawa A; Department of Obstetrics and Gynecology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan., Yoshida H; Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, 23-3 Matsuoka-Shimoaizuki, Eiheiji, Fukui, 910-1193, Japan., Shigeto S; Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan., Matsuda K; Department of Clinical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto, Japan., Akama TO; Department of Pharmacology, Kansai Medical University, Hirakata, Japan., Yoshida Y; Department of Obstetrics and Gynecology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan., Kobayashi M; Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, 23-3 Matsuoka-Shimoaizuki, Eiheiji, Fukui, 910-1193, Japan. motokoba@u-fukui.ac.jp.
المصدر: Human cell [Hum Cell] 2024 Jul; Vol. 37 (4), pp. 1184-1193. Date of Electronic Publication: 2024 Apr 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Japan NLM ID: 8912329 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1749-0774 (Electronic) Linking ISSN: 09147470 NLM ISO Abbreviation: Hum Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: 2011- : Tokyo : Springer
Original Publication: Tōkyō-to : Hito Saibō Kenkyūkai : Kanishobo, Shōwa 63-nen [1988]-
مواضيع طبية MeSH: Ovarian Neoplasms*/genetics , Ovarian Neoplasms*/pathology , Class I Phosphatidylinositol 3-Kinases*/genetics , Adenocarcinoma, Clear Cell*/genetics , Adenocarcinoma, Clear Cell*/pathology, Humans ; Female ; Cell Line, Tumor ; Middle Aged ; Transcription Factors/genetics ; Transcription Factors/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Nuclear Proteins/genetics ; Nuclear Proteins/metabolism ; Mutation/genetics ; Point Mutation/genetics ; Cell Proliferation/genetics ; Phosphatidylinositol 3-Kinases/metabolism ; Phosphatidylinositol 3-Kinases/genetics
مستخلص: A human ovarian clear cell carcinoma cell line was established from a 46-year-old Japanese woman. That line, designated MTC-22, has proliferated continuously for over 6 months in conventional RPMI 1640 medium supplemented with 10% foetal bovine serum and has been passaged over 50 times. MTC-22 doubling-time is ~ 18 h, which is much shorter than most ovarian clear cell carcinoma lines reported to date. Morphologically, MTC-22 cells exhibit polygonal shapes and proliferate to form a monolayer in a jigsaw puzzle-like arrangement without contact inhibition. Ultrastructurally, cells exhibit numerous intracytoplasmic glycogen granules and well-developed mitochondria. G-band karyotype analysis indicated that cells have a complex karyotype close to tetraploid. We observed that the expression pattern of a series of ovarian carcinoma-related molecules in MTC-22 cells was identical to that seen in the patient's tumour tissue. Notably, MTC-22 cells, and the patient's carcinoma tissue, expressed low-sulphated keratan sulphate recognised by R-10G and 294-1B1 monoclonal antibodies, a hallmark of non-mucinous ovarian carcinoma, and particularly of clear cell ovarian carcinoma. Moreover, characteristic point mutations-one in ARID1A, which encodes the AT-rich interaction domain containing protein 1A, and the other in PIK3CB, which encodes the catalytic subunit of phosphoinositide 3-kinase-were seen in the patient's tumour tissue and retained in MTC-22 cells. Collectively, these findings indicate that MTC-22 cells could serve as a valuable tool for investigating the pathophysiology of ovarian clear cell carcinoma, particularly that harbouring PIK3CB mutations, and for developing and validating new diagnostic and therapeutic approaches to this life-threatening malignancy.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 21H02702 Japan Society for the Promotion of Science
فهرسة مساهمة: Keywords: ARID1A; PIK3CB; Cell line; Clear cell carcinoma; Keratan sulphate; Ovary
المشرفين على المادة: EC 2.7.1.137 (Class I Phosphatidylinositol 3-Kinases)
EC 2.7.1.137 (PIK3CA protein, human)
0 (ARID1A protein, human)
0 (Transcription Factors)
0 (DNA-Binding Proteins)
0 (Nuclear Proteins)
EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
تواريخ الأحداث: Date Created: 20240404 Date Completed: 20240623 Latest Revision: 20240711
رمز التحديث: 20240711
مُعرف محوري في PubMed: PMC11194194
DOI: 10.1007/s13577-024-01058-x
PMID: 38573494
قاعدة البيانات: MEDLINE
الوصف
تدمد:1749-0774
DOI:10.1007/s13577-024-01058-x