دورية أكاديمية
Vegfc-expressing cells form heterotopic bone after musculoskeletal injury.
| العنوان: | Vegfc-expressing cells form heterotopic bone after musculoskeletal injury. |
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| المؤلفون: | Vishlaghi N; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA., Guo L; Department of Population and Data Sciences, University of Texas Southwestern, Dallas, TX, USA., Griswold-Wheeler D; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA., Sun Y; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA., Booker C; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA., Crossley JL; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA., Bancroft AC; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA., Juan C; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA., Korlakunta S; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA., Ramesh S; Department of Pathology, Johns Hopkins University, Baltimore, MD, USA., Pagani CA; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA., Xu L; Department of Population and Data Sciences, University of Texas Southwestern, Dallas, TX, USA., James AW; Department of Pathology, Johns Hopkins University, Baltimore, MD, USA., Tower RJ; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA., Dellinger M; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA. Electronic address: michael.dellinger@utsouthwestern.edu., Levi B; Department of Surgery, University of Texas Southwestern, Dallas, TX, USA. Electronic address: benjamin.levi@utsouthwestern.edu. |
| المصدر: | Cell reports [Cell Rep] 2024 Apr 23; Vol. 43 (4), pp. 114049. Date of Electronic Publication: 2024 Apr 03. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, c 2012- |
| مواضيع طبية MeSH: | Ossification, Heterotopic*/metabolism , Ossification, Heterotopic*/pathology , Ossification, Heterotopic*/genetics , Vascular Endothelial Growth Factor C*/metabolism , Vascular Endothelial Growth Factor C*/genetics , Lymphangiogenesis* , Mesenchymal Stem Cells*/metabolism, Animals ; Mice ; Lymphatic Vessels/metabolism ; Lymphatic Vessels/pathology ; Cell Differentiation ; Tenocytes/metabolism ; Osteogenesis ; Haploinsufficiency ; Mice, Inbred C57BL ; Disease Models, Animal ; Male |
| مستخلص: | Heterotopic ossification (HO) is a challenging condition that occurs after musculoskeletal injury and is characterized by the formation of bone in non-skeletal tissues. While the effect of HO on blood vessels is well established, little is known about its impact on lymphatic vessels. Here, we use a mouse model of traumatic HO to investigate the relationship between HO and lymphatic vessels. We show that injury triggers lymphangiogenesis at the injury site, which is associated with elevated vascular endothelial growth factor C (VEGF-C) levels. Through single-cell transcriptomic analyses, we identify mesenchymal progenitor cells and tenocytes as sources of Vegfc. We demonstrate by lineage tracing that Vegfc-expressing cells undergo osteochondral differentiation and contribute to the formation of HO. Last, we show that Vegfc haploinsufficiency results in a nearly 50% reduction in lymphangiogenesis and HO formation. These findings shed light on the complex mechanisms underlying HO formation and its impact on lymphatic vessels. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CP: Cell biology; CP: Developmental biology; MPCs; Vegfc, VEGFC; heterotopic ossification; lymphangiogenesis |
| المشرفين على المادة: | 0 (Vascular Endothelial Growth Factor C) 0 (vascular endothelial growth factor C, mouse) |
| تواريخ الأحداث: | Date Created: 20240404 Date Completed: 20240426 Latest Revision: 20240430 |
| رمز التحديث: | 20240430 |
| DOI: | 10.1016/j.celrep.2024.114049 |
| PMID: | 38573853 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2211-1247 |
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| DOI: | 10.1016/j.celrep.2024.114049 |