دورية أكاديمية
Circular extrachromosomal DNA in Euglena gracilis under normal and stress conditions.
| العنوان: | Circular extrachromosomal DNA in Euglena gracilis under normal and stress conditions. |
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| المؤلفون: | Gumińska N; Institute of Evolutionary Biology, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, 101 Żwirki i Wigury Street, 02-089 Warsaw, Poland; Laboratory of RNA Biology, International Institute of Molecular and Cell Biology in Warsaw, 4 Ks. Trojdena Street, 02-109 Warsaw, Poland. Electronic address: nguminska@iimcb.gov.pl., Hałakuc P; Institute of Evolutionary Biology, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, 101 Żwirki i Wigury Street, 02-089 Warsaw, Poland., Zakryś B; Institute of Evolutionary Biology, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, 101 Żwirki i Wigury Street, 02-089 Warsaw, Poland., Milanowski R; Institute of Evolutionary Biology, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, 101 Żwirki i Wigury Street, 02-089 Warsaw, Poland. Electronic address: r.milanowski@uw.edu.pl. |
| المصدر: | Protist [Protist] 2024 Jun; Vol. 175 (3), pp. 126033. Date of Electronic Publication: 2024 Mar 29. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: G. Fischer Country of Publication: Germany NLM ID: 9806488 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0941 (Electronic) Linking ISSN: 14344610 NLM ISO Abbreviation: Protist Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Jena : G. Fischer, 1998- |
| مواضيع طبية MeSH: | Euglena gracilis*/genetics , DNA, Circular*/genetics, DNA, Protozoan/genetics ; Ultraviolet Rays ; Stress, Physiological |
| مستخلص: | Extrachromosomal circular DNA (eccDNA) enhances genomic plasticity, augmenting its coding and regulatory potential. Advances in high-throughput sequencing have enabled the investigation of these structural variants. Although eccDNAs have been investigated in numerous taxa, they remained understudied in euglenids. Therefore, we examined eccDNAs predicted from Illumina sequencing data of Euglena gracilis Z SAG 1224-5/25, grown under optimal photoperiod and exposed to UV irradiation. We identified approximately 1000 unique eccDNA candidates, about 20% of which were shared across conditions. We also observed a significant enrichment of mitochondrially encoded eccDNA in the UV-irradiated sample. Furthermore, we found that the heterogeneity of eccDNA was reduced in UV-exposed samples compared to cells that were grown in optimal conditions. Hence, eccDNA appears to play a role in the response to oxidative stress in Euglena, as it does in other studied organisms. In addition to contributing to the understanding of Euglena genomes, our results contribute to the validation of bioinformatics pipelines on a large, non-model genome. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier GmbH. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Circular DNA; Euglena gracilis; Euglenozoa; eccDNA; mtDNA; rDNA |
| المشرفين على المادة: | 0 (DNA, Circular) 0 (DNA, Protozoan) |
| تواريخ الأحداث: | Date Created: 20240404 Date Completed: 20240511 Latest Revision: 20240511 |
| رمز التحديث: | 20240512 |
| DOI: | 10.1016/j.protis.2024.126033 |
| PMID: | 38574508 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1618-0941 |
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| DOI: | 10.1016/j.protis.2024.126033 |