دورية أكاديمية
Adipose-derived stem cell exosomes act as delivery vehicles of microRNAs in a dog model of chronic hepatitis.
| العنوان: | Adipose-derived stem cell exosomes act as delivery vehicles of microRNAs in a dog model of chronic hepatitis. |
|---|---|
| المؤلفون: | Zanolla I; Department of Medical Sciences, University of Ferrara, 44121, Ferrara, Italy., Trentini M; Department of Translational Medicine, University of Ferrara, 44121, Ferrara, Italy., Tiengo E; Department of Translational Medicine, University of Ferrara, 44121, Ferrara, Italy., Zanotti F; Department of Translational Medicine, University of Ferrara, 44121, Ferrara, Italy., Pusceddu T; Department of Translational Medicine, University of Ferrara, 44121, Ferrara, Italy., Rubini A; University of Bologna, 40126, Bologna, Italy., Rubini G; Ultravet Diagnostic, 40017, San Giovanni in Persiceto, Italy., Brugnoli F; Ultravet Diagnostic, 40017, San Giovanni in Persiceto, Italy., Licastro D; AREA Science Park, 34149, Trieste, Italy., Debortoli M; AREA Science Park, 34149, Trieste, Italy., Delogu LG; Department of Biomedical Sciences, University of Padua, 35122, Padua, Italy., Ferroni L; Department of Biomedical Sciences, University of Padua, 35122, Padua, Italy., Lovatti L; Department of Translational Medicine, University of Ferrara, 44121, Ferrara, Italy., Zavan B; Department of Translational Medicine, University of Ferrara, 44121, Ferrara, Italy. |
| المصدر: | Nanotheranostics [Nanotheranostics] 2024 Mar 09; Vol. 8 (3), pp. 298-311. Date of Electronic Publication: 2024 Mar 09 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Ivyspring International Publishers Country of Publication: Australia NLM ID: 101698994 Publication Model: eCollection Cited Medium: Internet ISSN: 2206-7418 (Electronic) Linking ISSN: 22067418 NLM ISO Abbreviation: Nanotheranostics Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Sydney, NSW : Ivyspring International Publishers, 2017- |
| مواضيع طبية MeSH: | MicroRNAs*/genetics , Exosomes*/genetics , Exosomes*/metabolism , Extracellular Vesicles*/metabolism, Dogs ; Animals ; Hepatitis, Chronic/metabolism ; Stem Cells/metabolism |
| مستخلص: | Exosomes are nanosized extracellular vesicles secreted by all cell types, including canine adipose-derived stem cells (cADSCs). By mediating intercellular communication, exosomes modulate the biology of adjacent and distant cells by transferring their cargo. In the present work after isolation and characterization of exosomes derived from canine adipose tissue, we treated the same canine donors affected by hepatopathies with the previously isolated exosomes. We hypothesize that cADSC-sourced miRNAs are among the factors responsible for a regenerative and anti-inflammatory effect in the treatment of hepatopathies in dogs, providing the clinical veterinary field with an effective and innovative cell-free therapy. Exosomes were isolated and characterized for size, distribution, surface markers, and for their miRNomic cargo by microRNA sequencing. 295 dogs affected with hepatopathies were treated and followed up for 6 months to keep track of their biochemical marker levels. Results confirmed that exosomes derived from cADSCs exhibited an average diameter of 91 nm, and positivity to 8 known exosome markers. The administration of exosomes to dogs affected by liver-associated inflammatory pathologies resulted in the recovery of the animal alongside the normalization of biochemical parameters of kidney function. In conclusion, cADSCs-derived exosomes are a promising therapeutic tool for treating inflammatory disorders in animal companions. Competing Interests: Competing Interests: The authors have declared that no competing interest exists. (© The author(s).) |
| References: | Biochim Biophys Acta. 2008 Nov;1779(11):735-42. (PMID: 18407843) Life Sci. 2019 Sep 15;233:116733. (PMID: 31394127) Theranostics. 2021 Mar 11;11(11):5248-5266. (PMID: 33859745) J Orthop Surg Res. 2022 Dec 26;17(1):567. (PMID: 36572886) Sci Rep. 2018 Jan 23;8(1):1419. (PMID: 29362496) Science. 2020 Feb 7;367(6478):. (PMID: 32029601) Front Endocrinol (Lausanne). 2018 Aug 03;9:402. (PMID: 30123182) Int J Mol Sci. 2020 Nov 09;21(21):. (PMID: 33182515) Stem Cells Transl Med. 2016 Jan;5(1):75-86. (PMID: 26582907) BMC Vet Res. 2020 Nov 2;16(1):417. (PMID: 33138803) Front Endocrinol (Lausanne). 2023 Mar 20;14:1129884. (PMID: 37020588) Vet J. 2019 Mar;245:77-81. (PMID: 30819430) Cancer Med. 2022 Oct;11(19):3687-3699. (PMID: 35441482) J Vet Intern Med. 2019 May;33(3):1173-1200. (PMID: 30844094) Eur J Pharmacol. 2019 Jan 15;843:307-315. (PMID: 30537490) Wound Repair Regen. 2013 May-Jun;21(3):382-94. (PMID: 23627495) Front Vet Sci. 2021 Oct 11;8:729929. (PMID: 34708100) J Orthop Translat. 2022 Jul 11;36:8-17. (PMID: 35891923) Int J Mol Sci. 2018 Dec 15;19(12):. (PMID: 30558283) J Cell Mol Med. 2022 Feb;26(3):624-635. (PMID: 34953040) J Transl Med. 2015 Sep 19;13:308. (PMID: 26386558) J Vet Sci. 2022 Sep;23(5):e79. (PMID: 36174983) Front Physiol. 2018 Jun 26;9:790. (PMID: 30013485) Pharmaceuticals (Basel). 2021 Aug 04;14(8):. (PMID: 34451863) BMB Rep. 2022 May;55(5):205-212. (PMID: 35410640) Int J Biol Sci. 2021 Jan 1;17(1):163-177. (PMID: 33390841) Cell Commun Signal. 2020 Sep 11;18(1):149. (PMID: 32917227) Biomedicines. 2022 Feb 10;10(2):. (PMID: 35203624) BMC Res Notes. 2012 Feb 13;5:92. (PMID: 22330228) Biotechnol Adv. 2018 Jan - Feb;36(1):328-334. (PMID: 29248680) Ann Transl Med. 2021 Apr;9(8):735. (PMID: 33987433) Hum Gene Ther. 2020 Mar;31(5-6):367-375. (PMID: 31950871) Stem Cell Res Ther. 2016 Aug 31;7(1):125. (PMID: 27581859) Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):4110-4118. (PMID: 31682476) Genome Biol. 2014 Jun 25;15(6):R83. (PMID: 24964909) Front Mol Neurosci. 2020 Apr 15;13:53. (PMID: 32351363) Cells. 2022 Dec 07;11(24):. (PMID: 36552714) Front Genet. 2022 Aug 23;13:915141. (PMID: 36081990) J Extracell Vesicles. 2018 Nov 23;7(1):1535750. (PMID: 30637094) Sci Rep. 2019 Sep 25;9(1):13848. (PMID: 31554838) Animals (Basel). 2022 Oct 10;12(19):. (PMID: 36230457) Stem Cell Res Ther. 2015 Jul 01;6:127. (PMID: 26129847) Cells. 2021 Aug 01;10(8):. (PMID: 34440728) Stem Cell Res Ther. 2018 Nov 21;9(1):320. (PMID: 30463593) Bone. 2021 Oct;151:116058. (PMID: 34144232) Int J Mol Sci. 2019 Feb 17;20(4):. (PMID: 30781550) Diagnostics (Basel). 2021 Feb 16;11(2):. (PMID: 33669374) Int J Mol Sci. 2023 Mar 22;24(6):. (PMID: 36983075) Pharmaceutics. 2022 Apr 21;14(5):. (PMID: 35631496) |
| فهرسة مساهمة: | Keywords: exosomes; extracellular nanovesicles; liver diseases; miRNA; regenerative medicine. |
| المشرفين على المادة: | 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20240405 Date Completed: 20240408 Latest Revision: 20240516 |
| رمز التحديث: | 20240516 |
| مُعرف محوري في PubMed: | PMC10988209 |
| DOI: | 10.7150/ntno.93064 |
| PMID: | 38577321 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2206-7418 |
|---|---|
| DOI: | 10.7150/ntno.93064 |