دورية أكاديمية
Plant growth-promoting and biocontrol traits of endophytic Bacillus licheniformis against soft rot causing Pythium myriotylum in ginger plant.
العنوان: | Plant growth-promoting and biocontrol traits of endophytic Bacillus licheniformis against soft rot causing Pythium myriotylum in ginger plant. |
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المؤلفون: | Alarjani KM; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia., Elshikh MS; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia. |
المصدر: | Journal of basic microbiology [J Basic Microbiol] 2024 Jul; Vol. 64 (7), pp. e2300643. Date of Electronic Publication: 2024 Apr 05. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 8503885 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-4028 (Electronic) Linking ISSN: 0233111X NLM ISO Abbreviation: J Basic Microbiol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: <2005->: Weinheim, Germany : Wiley-VCH Verlag Original Publication: Berlin : Akademie-Verlag, [1985- |
مواضيع طبية MeSH: | Pythium*/growth & development , Endophytes*/isolation & purification , Endophytes*/metabolism , Endophytes*/physiology , Plant Diseases*/microbiology , Plant Diseases*/prevention & control , Zingiber officinale*/microbiology , Zingiber officinale*/growth & development , Bacillus licheniformis*/growth & development , Bacillus licheniformis*/metabolism, Rhizome/microbiology ; Rhizome/growth & development ; Mycelium/growth & development ; Antibiosis ; Biological Control Agents/pharmacology ; Secondary Metabolism ; Chitinases/metabolism |
مستخلص: | Bacterial endophytes from plants harbor diverse metabolites that play major roles in biocontrol and improve plant growth. In this study, a total of 12 endophytic bacteria were isolated from the ginger rhizome. The strain K3 was highly effective in preventing mycelia growth of Pythium myriotylum (78.5 ± 1.5% inhibition) in dual culture. The cell-free extract (2.5%) of endophyte K3 inhibited 76.3 ± 4.8% mycelia growth, and 92.4 ± 4.2% inhibition was observed at a 5% sample concentration. The secondary metabolites produced by Bacillus licheniformis K3 showed maximum activity against Pseudomonas syringae (24 ± 1 mm zone of inhibition) and Xanthomonas campestris (28 ± 3 mm zone of inhibition). The strain K3 produced 28.3 ± 1.7 IU mL -1 protease, 28.3 ± 1.7 IU mL -1 cellulase, and 2.04 ± 0.13 IU mL -1 chitinase, respectively. The ginger rhizome treated with K3 in the greenhouse registered 53.8 ± 1.4% soft rot incidence, and the streptomycin-treated pot registered 78.3 ± 1.7% disease incidence. The selected endophyte K3 improved ascorbate peroxidase (1.37 ± 0.009 µmole ASC min -1 mg -1 protein), catalase (8.7 ± 0.28 µmole min -1 mg -1 protein), and phenylalanine ammonia-lyase (26.2 ± 0.99 Umg -1 ) in the greenhouse. In addition, K3 treatment in the field trial improved rhizome yield (730 ± 18.4 g) after 180 days (p < 0.01). The shoot length was 46 ± 8.3 cm in K3-treated plants, and it was about 31% higher than the control treatment (p < 0.01). The lytic enzyme-producing and growth-promoting endophyte is useful in sustainable crop production through the management of biotic stress. (© 2024 Wiley‐VCH GmbH.) |
References: | Taheri E, Tarighi S, Taheri P. An endophytic bacterium with biocontrol activity against important wheat pathogens. Biol Control. 2023;183:105243. Wang S, Wang J, Zhou Y, Huang Y, Tang X. Prospecting the plant growth–promoting activities of endophytic bacteria Franconibacter sp. YSD YN2 isolated from Cyperus esculentus L. var. sativus leaves. Ann Microbiol. 2022;72:1–5. Cui L, Yang C, Wang Y, Ma T, Cai F, Wei L, et al. Potential of an endophytic bacteria Bacillus amyloliquefaciens 3–5 as biocontrol agent against potato scab. Microb Pathog. 2022;163:105382. Bódalo A, Borrego R, Garrido C, Bolivar‐Anillo HJ, Cantoral JM, Vela‐Delgado MD, et al. In vitro studies of endophytic bacteria isolated from ginger (Zingiber officinale) as potential plant‐growth‐promoting and biocontrol agents against Botrytis cinerea and Colletotrichum acutatum. Plants. 2023;12:4032. Paparu P, Acur A, Kato F, Acam C, Nakibuule J, Musoke S, et al. Prevalence and incidence of four common bean root rots in Uganda. Exp Agric. 2018;54:888–900. Zou L, Wang Q, Wu R, Zhang Y, Wu Q, Li M, et al. Biocontrol and plant growth promotion potential of endophytic Bacillus subtilis JY‐7‐2L on Aconitum carmichaelii Debx. Front Microbiol. 2023;13:1059549. Yasmin S, Rahman Bakar MA, Malik KA, Hafeez FY. Isolation, characterization and beneficial effects of rice associated plant growth promoting bacteria from Zanzibar soils. J Basic Microbiol. 2004;44:241–252. Adesemoye AO, Torbert HA, Kloepper JW. Plant growth‐promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microb Ecol. 2009;58:921–929. Do QT. Antagonistic activities of endophytic bacteria isolated from rice roots against the fungus Magnaporthe oryzae, a causal of rice blast disease. Egypt J Biol Pest Control. 2022;32:69. Zhang S, Kou X, Zhao H, Mak KK, Balijepalli MK, Pichika MR. Zingiber officinale var. rubrum: red ginger's medicinal uses. Molecules. 2022;27:775. Yadav D, Gaurav H, Yadav R, Waris R, Afzal K, Chandra Shukla A. A comprehensive review on soft rot disease management in ginger (Zingiber officinale) for enhancing its pharmaceutical and industrial values. Heliyon. 2023;9:e18337. Al‐Hussini HS, Al‐Rawahi AY, Al‐Marhoon AA, Al‐Abri SA, Al‐Mahmooli IH, Al‐Sadi AM, et al. Biological control of damping‐off of tomato caused by Pythium aphanidermatum by using native antagonistic rhizobacteria isolated from Omani soil. J Plant Pathol. 2019;101:315–322. Kumar A, Shukla R, Singh P, Prasad CS, Dubey NK. Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against post harvest fungal infestation of food commodities. Innovative Food Sci Emerging Technol. 2008;9:575–580. Pandey DK, Chandra H, Tripathi NN. Volatile fungitoxic activity of some higher plants with special reference to that of Callistemon lanceolatus DC. J Phytopath. 1982;105:175–182. Vijayaraghavan P, Arun A, Al‐Dhabi NA, Vincent SGP, Arasu MV, Choi KC. Novel Bacillus subtilis IND19 cell factory for the simultaneous production of carboxy methyl cellulase and protease using cow dung substrate in solid‐substrate fermentation. Biotechnol Biofuels. 2016;9:73. Devi KA, Pandey G, Rawat AKS, Sharma GD, Pandey P. The endophytic symbiont ‐ Pseudomonas aeruginosa stimulates the antioxidant activity and growth of Achyranthes aspera L. Front Microbiol. 2017;8:1897. Orhan F. Alleviation of salt stress by halotolerant and halophilic plant growth‐promoting bacteria in wheat (Triticum aestivum). Braz J Microbiol. 2016;47:621–627. Goswami D, Dhandhukia P, Patel P, Thakker JN. Screening of PGPR from saline desert of kutch: growth promotion in Arachis hypogea by Bacillus licheniformis A2. Microbiol Res. 2014;169:66–75. Li L, Mohamad OAA, Ma J, Friel AD, Su Y, Wang Y, et al. Synergistic plant–microbe interactions between endophytic bacterial communities and the medicinal plant Glycyrrhiza uralensis F. Antonie Van Leeuwenhoek. 2018;111:1735–1748. Ferreira SC, Nakasone AK, do Nascimento SMC, de Oliveira DA, Siqueira AS, Cunha EFM, et al. Isolation and characterization of cassava root endophytic bacteria with the ability to promote plant growth and control the in vitro and in vivo growth of Phytopythium sp. Physiol Mol Plant Path. 2021;116:101709. Chen GX, Asada K. Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol. 1989;30:987–998. Cheng GW, Breen PJ. Activity of phenylalanine ammonia‐lyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. J Am Soc Hortic Sci. 1991;116:865–869. Wang L, Xi N, Lang D, Zhou L, Zhang Y, Zhang X. Potential biocontrol and plant growth promotion of an endophytic bacteria isolated from Glycyrrhiza uralensis seeds. Egypt J Biol Pest Control. 2022;32:55. Lopes R, Tsui S, Gonçalves PJRO, de Queiroz MV. A look into a multifunctional toolbox: endophytic Bacillus species provide broad and underexploited benefits for plants. World J Microbiol Biotechnol. 2018;34:94. Ait‐Ouakrim EH, Chakhchar A, El Modafar C, Douira A, Amir S, Ibnsouda‐Koraichi S, et al. Valorization of Moroccan phosphate sludge through isolation and characterization of phosphate solubilizing bacteria and assessment of their growth promotion effect on Phaseolus vulgaris. Waste Biomass Valorization. 2023;14:2673–2690. Boss BL, Wanees AE, Zaslow SJ, Normile TG, Izquierdo JA. Comparative genomics of the plant‐growth promoting bacterium Sphingobium sp. strain AEW4 isolated from the rhizosphere of the beachgrass Ammophila breviligulata. BMC Genomics. 2022;23:508. Dutta J, Handique PJ, Thakur D. Assessment of culturable tea rhizobacteria isolated from tea estates of Assam, India for growth promotion in commercial tea cultivars. Front Microbiol. 2015;6:1252. Baard V, Bakare OO, Daniel AI, Nkomo M, Gokul A, Keyster M, et al. Biocontrol potential of Bacillus subtilis and Bacillus tequilensis against four Fusarium species. Pathogens. 2023;12:254. Singh DP, Singh V, Gupta VK, Shukla R, Prabha R, Sarma BK, et al. Microbial inoculation in rice regulates antioxidative reactions and defense related genes to mitigate drought stress. Sci Rep. 2020;10:4818. Mittler R. ROS are good. Trends Plant Sci. 2017;22:11–19. Dinesh R, Anandaraj M, Kumar A, Bini YK, Subila KP, Aravind R. Isolation, characterization, and evaluation of multi‐trait plant growth promoting rhizobacteria for their growth promoting and disease suppressing effects on ginger. Microbiol Res. 2015;173:34–43. Jabborova D, Davranov K, Jabbarov Z, Bhowmik SN, Ercisli S, Danish S, et al. Dual inoculation of plant growth‐promoting Bacillus endophyticus and Funneliformis mosseae improves plant growth and soil properties in ginger. ACS Omega. 2022;7:34779–34788. Peter P, Divakaran K, Kaniyala H, Nair AR. Endophytic Bacillus spp. from Zingiber zerumbet rhizome, rhizosphere and axenic cultures: characterisation and identification of isolates with plant growth promoting activities and antagonism to soft‐rot causative Pythium myriotylum. Arch Phytopathol Plant Prot. 2021;54:2007–2022. |
معلومات مُعتمدة: | RSP2024R185 King Saud University |
فهرسة مساهمة: | Keywords: disease control; endophyte; lytic enzymes; pathogenic fungi; soft‐rot |
المشرفين على المادة: | 0 (Biological Control Agents) EC 3.2.1.14 (Chitinases) |
تواريخ الأحداث: | Date Created: 20240405 Date Completed: 20240703 Latest Revision: 20240703 |
رمز التحديث: | 20240703 |
DOI: | 10.1002/jobm.202300643 |
PMID: | 38578065 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1521-4028 |
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DOI: | 10.1002/jobm.202300643 |