دورية أكاديمية
Comparison of salpingectomy and tubal detorsion procedures after experimental ischemia-reperfusion injury in a rat fallopian tube model: biochemical and histopathological evaluation.
| العنوان: | Comparison of salpingectomy and tubal detorsion procedures after experimental ischemia-reperfusion injury in a rat fallopian tube model: biochemical and histopathological evaluation. |
|---|---|
| المؤلفون: | Koyan Karadeniz GN; Department of Gynaecology and Obstetrics, University of Health Sciences, Basaksehir Cam and Sakura City Hospital, Istanbul, Turkey. Electronic address: gizkyn@gmail.com., Karadeniz O; Department of Gynaecology and Obstetrics, Arnavutkoy State Hospital, Istanbul, Turkey., Bulutlar E; Department of Obstetrics and Gynecology, Health Sciences University, Zeynep Kamil Women and Children's Diseases Training and Research Hospital, Istanbul, Turkey., Yilmaz B; Department of Gynaecology and Obstetrics, University of Health Sciences, Basaksehir Cam and Sakura City Hospital, Istanbul, Turkey., Gedikbasi A; Department of Biochemistry, Faculty of Medicine, Istanbul University, Istanbul, Turkey., Arslan HS; Department of Pathology, University of Health Sciences, Basaksehir Cam ve Sakura City Hospital, Istanbul, Turkey., Cetin BA; Department of Gynaecology and Obstetrics, University of Health Sciences, Basaksehir Cam and Sakura City Hospital, Istanbul, Turkey., Polat İ; Department of Gynaecology and Obstetrics, University of Health Sciences, Basaksehir Cam and Sakura City Hospital, Istanbul, Turkey. |
| المصدر: | F&S science [F S Sci] 2024 May; Vol. 5 (2), pp. 195-203. Date of Electronic Publication: 2024 Apr 04. |
| نوع المنشور: | Journal Article; Comparative Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc Country of Publication: United States NLM ID: 101765857 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-335X (Electronic) Linking ISSN: 2666335X NLM ISO Abbreviation: F S Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [New York] : Elsevier Inc., [2020]- |
| مواضيع طبية MeSH: | Salpingectomy* , Reperfusion Injury*/pathology , Reperfusion Injury*/metabolism , Rats, Sprague-Dawley* , Fallopian Tubes*/pathology , Fallopian Tubes*/surgery , Fallopian Tubes*/injuries, Animals ; Female ; Rats ; Disease Models, Animal ; Anti-Mullerian Hormone/blood ; Malondialdehyde/metabolism ; Malondialdehyde/blood ; Superoxide Dismutase/metabolism ; Glutathione Peroxidase/metabolism ; Oxidative Stress ; Catalase/metabolism |
| مستخلص: | Objective: To compare salpingectomy and detorsion procedures and investigate the biochemical and histopathological changes in the fallopian tubes in the experimentally isolated fallopian tube torsion model in rats. Design: Experimental study. Setting: Experimental surgery laboratory in a training and research hospital. Animal(s): Twenty-seven Sprague-Dawley rats in the reproductive period. Intervention(s): Group 1, control group (n = 6); group 2, bilateral total salpingectomy group after 4 hours of tubal ischemia (n = 7); group 3: 4 hours of bilateral tubal ischemia plus 1 week of reperfusion (n = 7); and group 4, 4-hour period of bilateral tubal ischemia plus 30 days of reperfusion (n = 7). A 22-gauge catheter was administered before and after surgery using methylene blue through the uterine horn of the rat to evaluate tubal patency. Main Outcome Measure(s): Preoperative and postoperative serum antimüllerian hormone (AMH) levels, histopathological examination of the rat tuba uterine and histopathological damage scores, antioxidant compounds (superoxide dismutase [SOD], catalase, and glutathione peroxidase [GSH-Px]), and oxidative stress end product levels (malondialdehyde [MDA] and 8-hydroxy-2'-deoxyguanosine [8-OHdG]). Result(s): Although a significant difference was observed in the tissue SOD, GSH-Px, MDA, and 8-OHdG values, no significant difference was observed between the groups in serum samples. The tissue SOD and tissue GSH-Px levels in group 2 significantly decreased, and a significant increase was observed in the tissue MDA and 8-OHdG values in group 2. Among the histopathological parameters, epithelial changes, vascular congestion, and the total fallopian tube mean damage score of 4 showed a significant decrease in group 4. When the methylene blue transitions before and after ischemia-reperfusion injury were compared, the values of the methylene blue transition after ischemia-reperfusion injury in groups 2-4 significantly decreased. When the serum AMH levels were analyzed, the postoperative AMH value in group 2 significantly increased. Conclusion(s): This study reveals that biochemical and histopathological improvement is observed in the fallopian tube tissues gradually when the detorsion procedure is performed for the necrotized tubal tissue instead of salpingectomy. Although there is restoration of epithelial integrity after reperfusion, tubal passage remains absent. Clinical Trial Registration Number: This study was approved by the Local Ethics Committee for Animal Experiments of the Health Sciences University, Istanbul Hamidiye Medicine Faculty (approval number 27.05.2022-9269). The study followed the ethics standards recommended by the Declaration of Helsinki. Competing Interests: Declaration of Interests G.N.K.K. has nothing to disclose. O.K. has nothing to disclose. E.B. has nothing to disclose. B.Y. has nothing to disclose. A.G. has nothing to disclose. H.S.A. has nothing to disclose. B.A.C. has nothing to disclose. İ.P. has nothing to disclose. (Copyright © 2024 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Detorsion; ischemia; reperfusion; salpingectomy; tubal torsion |
| المشرفين على المادة: | 80497-65-0 (Anti-Mullerian Hormone) 4Y8F71G49Q (Malondialdehyde) EC 1.15.1.1 (Superoxide Dismutase) EC 1.11.1.9 (Glutathione Peroxidase) EC 1.11.1.6 (Catalase) |
| تواريخ الأحداث: | Date Created: 20240405 Date Completed: 20240523 Latest Revision: 20240530 |
| رمز التحديث: | 20240530 |
| DOI: | 10.1016/j.xfss.2024.03.003 |
| PMID: | 38580179 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2666-335X |
|---|---|
| DOI: | 10.1016/j.xfss.2024.03.003 |