دورية أكاديمية
أعرض في EDS

Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes.

التفاصيل البيبلوغرافية
العنوان: Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes.
المؤلفون: Kandil OM; Department of Animal Reproduction & Artificial Insemination, Veterinary Research Institute, National Research Centre, Cairo, Egypt. om.kandil@nrc.sci.eg.; Accredited (ISO 17025) Embryo and Genetic Resources Conservation Bank in National Research Centre (NRC), Cairo, Egypt. om.kandil@nrc.sci.eg., Rahman SMAE; Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt., Ali RS; Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt., Ismail EA; Department of Animal Reproduction & Artificial Insemination, Veterinary Research Institute, National Research Centre, Cairo, Egypt., Ibrahim NM; Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt.
المصدر: Reproductive biology and endocrinology : RB&E [Reprod Biol Endocrinol] 2024 Apr 05; Vol. 22 (1), pp. 39. Date of Electronic Publication: 2024 Apr 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101153627 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-7827 (Electronic) Linking ISSN: 14777827 NLM ISO Abbreviation: Reprod Biol Endocrinol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2003-
مواضيع طبية MeSH: Buffaloes* , Melatonin*/pharmacology, Animals ; Oocytes ; Cryopreservation/veterinary ; Vitrification ; Fertilization in Vitro
مستخلص: Background: In livestock breeding, oocyte cryopreservation is crucial for preserving and transferring superior genetic traits. This study was conducted to examine the additional effect of melatonin to maturation and vitrification media on the in vitro developmental capacity, mitochondrial distribution, and intensity of buffalo oocytes. The study involved obtaining ovaries from a slaughterhouse and conducting two phases. In the first phase, high-quality oocytes were incubated in a maturation medium with or without 10 -9 M melatonin for 22 h (at 38.5°C in 5% CO 2 ). Matured oocytes were fertilized in vitro and cultured in SOF media for seven days. In the second phase, vitrified in vitro matured oocytes were stored in vitrified media (basic media (BM) containing a combination of cryoprotectants (20% Ethyl Glycol and 20% Dimethyl sulfoxide), with or without melatonin, and then stored in liquid nitrogen. Normal vitrified/thawed oocytes were fertilized in vitro and cultured as described. Finally, the matured oocytes from the fresh and vitrified/thawed groups, both with and without melatonin, were stained using DAPI and Mitotracker red to detect their viability (nuclear maturation), mitochondrial intensity, and distribution using a confocal microscope. The study found that adding 10 -9 M melatonin to the maturation media significantly increased maturation (85.47%), fertilization rate (84.21%)cleavage (89.58%), and transferable embryo (48.83%) rates compared to the group without melatonin (69.85%,79.88%, 75.55%, and 37.25% respectively). Besides that, the addition of melatonin to the vitrification media improved the recovery rate of normal oocytes (83.75%), as well as the cleavage (61.80%) and transferable embryo (27.00%) rates when compared to the vitrified TCM group (67.46%, 51.40%, and 17.00%, respectively). The diffuse mitochondrial distribution was higher in fresh with melatonin (TCM + Mel) (80%) and vitrified with melatonin (VS2 + Mel groups) (76.70%), Furthermore, within the same group, while the mitochondrial intensity was higher in the TCM + Mel group (1698.60) than other group. In conclusion, Melatonin supplementation improves the developmental competence and mitochondrial distribution in buffalo oocytes in both cases(in vitro maturation and vitrification).
(© 2024. The Author(s).)
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فهرسة مساهمة: Keywords: Buffalo; In-vitro embryo development; Melatonin; Mitochondria; Vitrification
المشرفين على المادة: JL5DK93RCL (Melatonin)
تواريخ الأحداث: Date Created: 20240405 Date Completed: 20240408 Latest Revision: 20240408
رمز التحديث: 20240408
مُعرف محوري في PubMed: PMC10996257
DOI: 10.1186/s12958-024-01209-7
PMID: 38580962
قاعدة البيانات: MEDLINE
الوصف
تدمد:1477-7827
DOI:10.1186/s12958-024-01209-7