دورية أكاديمية
Integrated analysis of intestinal microbiota and transcriptome reveals that a coordinated interaction of the endocrine, immune system and gut microbiota response to heat stress in Litopenaeus vannamei.
| العنوان: | Integrated analysis of intestinal microbiota and transcriptome reveals that a coordinated interaction of the endocrine, immune system and gut microbiota response to heat stress in Litopenaeus vannamei. |
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| المؤلفون: | Liao G; College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China., Wang W; College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China., Yu J; College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China., Li J; College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China., Yan Y; College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China., Liu H; College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China., Chen B; Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China., Fan L; College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China. Electronic address: fanlanfen@scau.edu.cn. |
| المصدر: | Developmental and comparative immunology [Dev Comp Immunol] 2024 Jul; Vol. 156, pp. 105176. Date of Electronic Publication: 2024 Apr 04. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 7708205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0089 (Electronic) Linking ISSN: 0145305X NLM ISO Abbreviation: Dev Comp Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown Ny : Elsevier Science Original Publication: Elmsford, N. Y., Pergamon Press., 1977-2004. |
| مواضيع طبية MeSH: | Penaeidae*/immunology , Penaeidae*/microbiology , Penaeidae*/genetics , Heat-Shock Response*/genetics , Heat-Shock Response*/immunology , Gastrointestinal Microbiome*/immunology , Transcriptome*, Animals ; Intestines/immunology ; Intestines/microbiology ; Immune System/metabolism ; Immune System/immunology ; Gene Expression Profiling ; Hepatopancreas/immunology ; Hepatopancreas/metabolism ; Arthropod Proteins/metabolism ; Arthropod Proteins/genetics ; Antioxidants/metabolism |
| مستخلص: | Due to the ongoing global warming, the risk of heatwaves in the oceans is continuously increasing while our understanding of the physiological response of Litopenaeus vannamei under extreme temperature conditions remains limited. Therefore, this study aimed to evaluate the physiological responses of L. vannamei under heat stress. Our results indicated that as temperature rose, the structure of intestinal and hepatopancreatic tissues was damaged sequentially. Activity of immune-related enzymes (acid phosphatase/alkaline phosphatase) initially increased before decreased, while antioxidant enzymes (superoxide dismutase and glutathione-S transferase) activity and malondialdehyde content increased with rising temperature. In addition, the total antioxidant capacity decreased with rising temperature. With the rising temperature, there was a significant increase in the expression of caspase-3, heat shock protein 70, lipopolysaccharide-induced tumor necrosis factor-α, transcriptional enhanced associate domain and yorkie in intestinal and hepatopancreatic tissues. Following heat stress, the number of potentially beneficial bacteria (Rhodobacteraceae and Gemmonbacter) increased which maintain balance and promote vitamin synthesis. Intestinal transcriptome analysis revealed 852 differentially expressed genes in the heat stress group compared with the control group. KEGG functional annotation results showed that the endocrine system was the most abundant in Organismal systems followed by the immune system. These results indicated that heat stress leads to tissue damage in shrimp, however the shrimp may respond to stress through a coordinated interaction strategy of the endocrine system, immune system and gut microbiota. This study revealed the response mechanism of L. vannamei to acute heat stress and potentially provided a theoretical foundation for future research on shrimp environmental adaptations. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Heat stress; Intestinal microbiota; Litopenaeus vannamei; Oxidative stress; Transcriptome |
| المشرفين على المادة: | 0 (Arthropod Proteins) 0 (Antioxidants) |
| تواريخ الأحداث: | Date Created: 20240406 Date Completed: 20240501 Latest Revision: 20240501 |
| رمز التحديث: | 20240502 |
| DOI: | 10.1016/j.dci.2024.105176 |
| PMID: | 38582249 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0089 |
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| DOI: | 10.1016/j.dci.2024.105176 |