دورية أكاديمية
The influence of cation exchange on the possible mechanism of erionite toxicity: A synchrotron-based micro-X-ray fluorescence study on THP-1-derived macrophages exposed to erionite-Na.
| العنوان: | The influence of cation exchange on the possible mechanism of erionite toxicity: A synchrotron-based micro-X-ray fluorescence study on THP-1-derived macrophages exposed to erionite-Na. |
|---|---|
| المؤلفون: | Raneri S; CNR-ICCOM, National Research Council, Institute of Chemistry and OrganoMetallic Compounds, Via G. Moruzzi, 1, 56124, Pisa, Italy., Gianoncelli A; Elettra Sincrotrone Trieste, Strada Statale 14, Km 163.5 in Area Science Park, 34149 Basovizza Trieste, Italy., Bonanni V; Elettra Sincrotrone Trieste, Strada Statale 14, Km 163.5 in Area Science Park, 34149 Basovizza Trieste, Italy., Mirata S; Department of Earth, Environment and Life Sciences, University of Genova, Corso Europa 26, 16132, Genova, Italy., Scarfì S; Department of Earth, Environment and Life Sciences, University of Genova, Corso Europa 26, 16132, Genova, Italy. Electronic address: soniascarfi@unige.it., Fornasini L; Department of Mathematical, Physical and Computer Sciences, University of Parma, Parco Area delle Scienze 7/A, 43124, Parma, Italy., Bersani D; Department of Mathematical, Physical and Computer Sciences, University of Parma, Parco Area delle Scienze 7/A, 43124, Parma, Italy., Baroni D; Istituto di Biofisica, CNR, Via De Marini 6, 15149, Genova, Italy., Picco C; Istituto di Biofisica, CNR, Via De Marini 6, 15149, Genova, Italy., Gualtieri AF; Department of Chemical and Geological Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125, Modena, Italy. |
| المصدر: | Environmental research [Environ Res] 2024 Jul 01; Vol. 252 (Pt 1), pp. 118878. Date of Electronic Publication: 2024 Apr 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0147621 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0953 (Electronic) Linking ISSN: 00139351 NLM ISO Abbreviation: Environ Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Amsterdam : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Zeolites*/toxicity , Zeolites*/chemistry , Macrophages*/drug effects , Synchrotrons*, Humans ; THP-1 Cells ; Cations ; Spectrometry, X-Ray Emission ; Phagocytosis/drug effects ; Calcium/metabolism ; Sodium |
| مستخلص: | Fibrous erionite is the only zeolite classified as Group 1 carcinogen by the International Agency for Research on Cancer (IARC). Carcinogenesis induced by erionite is thought to involve several factors as biopersistence, the iron role and cation exchange processes. To better understand these mechanisms, a detailed investigation at the micro scale was performed, collecting elemental information on iron and cation release and their distribution in biological systems by synchrotron micro-X-ray fluorescence mapping (SR-micro-XRF) and synchrotron micro-X-ray absorption spectroscopy (SR-micro-XANES) at the TwinMic beamline (Elettra synchrotron) and at the ID21 beamline of the European Synchrotron Radiation Facility (ESRF). By microscopy and chemical mapping, highly detailed maps of the chemical and morphological interaction of biological systems with fibres could be produced. In detail, THP-1 cell line derived macrophages, used as in vitro model, were analysed during erionite-Na phagocytosis at different time intervals, after single dose exposure. For comparison, cellular fluorescent probes were also used to evaluate the intracellular free sodium and calcium concentrations. Synchrotron analyses visualised the spatial distribution of both fibre and mineral particle associated metals during the phagocytosis, describing the mechanism of internalisation of erionite-Na and its accessory mineral phases. The intracellular distribution of metals and other cations was mapped to evaluate metal release, speciation changes and/or cation exchange during phagocytosis. The fluorescent probes complemented microchemical data clarifying, and confirming, the cation distribution observed in the SR-micro-XRF maps. The significant cytoplasmic calcium decrease, and the concomitant sodium increase, after the fibre phagocytosis seemed due to activation of plasma membrane cations exchangers triggered by the internalisation while, surprisingly, the ion-exchange capacity of erionite-Na could play a minor role in the disruption of the two cations intracellular homeostasis. These results help to elucidate the role of cations in the toxicity of erionite-treated THP-1 macrophages and add knowledge to its carcinogenicity process. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Alessandro Gualtieri reports financial support was provided by Italian Ministry of University and Research. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cation exchange; Erionite; Iron; Synchrotron radiation; Toxicity |
| المشرفين على المادة: | 1318-02-1 (Zeolites) 12510-42-8 (erionite) 0 (Cations) SY7Q814VUP (Calcium) 9NEZ333N27 (Sodium) |
| تواريخ الأحداث: | Date Created: 20240406 Date Completed: 20240606 Latest Revision: 20240606 |
| رمز التحديث: | 20240606 |
| DOI: | 10.1016/j.envres.2024.118878 |
| PMID: | 38582417 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0953 |
|---|---|
| DOI: | 10.1016/j.envres.2024.118878 |