دورية أكاديمية
INOS ablation promotes corneal wound healing via activation of Akt signaling.
| العنوان: | INOS ablation promotes corneal wound healing via activation of Akt signaling. |
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| المؤلفون: | Sun Z; Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, 300070, Tianjin, China., Lu K; Tianjin Eye Hospital, Tianjin Key Lab of Ophthalmology and Visual Science, 300070, Tianjin, China., He Q; Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, 300070, Tianjin, China., Tang Y; Qingdao State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, 266071, Qingdao, China., Li H; Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, 300070, Tianjin, China., Pazo EE; Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, 300070, Tianjin, China., Hu L; Basic Medical College, Immunology Department, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Medical University, Tianjin, 300070, China. Electronic address: lizhihu@tmu.edu.cn., Wei R; Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, 300070, Tianjin, China. Electronic address: rwei@tmu.edu.cn. |
| المصدر: | Experimental eye research [Exp Eye Res] 2024 Jun; Vol. 243, pp. 109886. Date of Electronic Publication: 2024 Apr 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 0370707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0007 (Electronic) Linking ISSN: 00144835 NLM ISO Abbreviation: Exp Eye Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Academic Press Original Publication: London. |
| مواضيع طبية MeSH: | Cell Movement*/physiology , Corneal Injuries*/metabolism , Corneal Injuries*/pathology , Disease Models, Animal* , Epithelium, Corneal*/metabolism , Nitric Oxide Synthase Type II*/metabolism , Proto-Oncogene Proteins c-akt*/metabolism , Signal Transduction*/physiology , Wound Healing*/physiology, Animals ; Humans ; Male ; Mice ; Blotting, Western ; Mice, Inbred C57BL ; Mice, Knockout ; Nitric Oxide/metabolism ; Phosphorylation |
| مستخلص: | Corneal injury leads to impaired normal structure of the cornea. Improving the wound healing process in epithelial cells significantly contributes to ocular damage treatments. Here, we aimed to investigate the potential mechanisms of nitric oxide (NO) and its mediator, inducible nitric oxide synthase (iNOS), in the process of corneal wound healing. We established a corneal injury model of iNOS -/- mice, and treated human corneal epithelial cell lines (HCE-2) with the iNOS inhibitor L-INL, with or without NO replenishment by supplying sodium nitroferricyanide dihydrate (SNP). Our findings showed that inhibition of NO/iNOS accelerated corneal repair, enhanced uPAR (a receptor protein indicating the migration ability), and improved epithelial cell migration. Furthermore, NO/iNOS ablation activated Akt phosphorylation, reduced neutrophil marker protein MPO expression, and downregulated the transcription of inflammation cytokines CXCL-1, CXCL-2, IL-1β, IL-6, and TNF-α. However, the protective effects of NO/iNOS inhibition are significantly reduced by NO replenishment when treated with SNP. Therefore, we confirmed that inhibiting NO/iNOS improved the corneal wound healing by facilitating epithelial cell migration and reducing inflammatory reactions, which might be related to the activation of the Akt signaling pathway. Competing Interests: Declaration of competing interest The authors declare no conflict of interest. (Copyright © 2024. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Akt signaling pathway; Corneal wound healing; Epithelial cell migration; Inducible nitric oxide synthase; Nitric oxide |
| المشرفين على المادة: | 31C4KY9ESH (Nitric Oxide) EC 1.14.13.39 (Nitric Oxide Synthase Type II) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) EC 1.14.13.39 (NOS2 protein, human) EC 1.14.13.39 (Nos2 protein, mouse) |
| تواريخ الأحداث: | Date Created: 20240407 Date Completed: 20240519 Latest Revision: 20240528 |
| رمز التحديث: | 20240528 |
| DOI: | 10.1016/j.exer.2024.109886 |
| PMID: | 38583755 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0007 |
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| DOI: | 10.1016/j.exer.2024.109886 |