دورية أكاديمية
أعرض في EDS

Analysis of twelve genomes of the bacterium Kerstersia gyiorum from brown-throated sloths ( Bradypus variegatus ), the first from a non-human host.

التفاصيل البيبلوغرافية
العنوان: Analysis of twelve genomes of the bacterium Kerstersia gyiorum from brown-throated sloths ( Bradypus variegatus ), the first from a non-human host.
المؤلفون: Carhuaricra-Huaman D; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil., Gonzalez IHL; Coordenadoria de Fauna Silvestre, Secretaria do Meio Ambiente, São Paulo, SP, Brazil., Ramos PL; Coordenadoria de Fauna Silvestre, Secretaria do Meio Ambiente, São Paulo, SP, Brazil., da Silva AM; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil., Setubal JC; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
المصدر: PeerJ [PeerJ] 2024 Apr 04; Vol. 12, pp. e17206. Date of Electronic Publication: 2024 Apr 04 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: PeerJ Inc Country of Publication: United States NLM ID: 101603425 Publication Model: eCollection Cited Medium: Internet ISSN: 2167-8359 (Electronic) Linking ISSN: 21678359 NLM ISO Abbreviation: PeerJ Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Corte Madera, CA : PeerJ Inc.
مواضيع طبية MeSH: Sloths*/genetics , Alcaligenaceae*/genetics, Animals ; Phylogeny ; Brazil
مستخلص: Kerstersia gyiorum is a Gram-negative bacterium found in various animals, including humans, where it has been associated with various infections. Knowledge of the basic biology of K. gyiorum is essential to understand the evolutionary strategies of niche adaptation and how this organism contributes to infectious diseases; however, genomic data about K. gyiorum is very limited, especially from non-human hosts. In this work, we sequenced 12 K. gyiorum genomes isolated from healthy free-living brown-throated sloths ( Bradypus variegatus ) in the Parque Estadual das Fontes do Ipiranga (São Paulo, Brazil), and compared them with genomes from isolates of human origin, in order to gain insights into genomic diversity, phylogeny, and host specialization of this species. Phylogenetic analysis revealed that these K. gyiorum strains are structured according to host. Despite the fact that sloth isolates were sampled from a single geographic location, the intra-sloth K. gyiorum diversity was divided into three clusters, with differences of more than 1,000 single nucleotide polymorphisms between them, suggesting the circulation of various K. gyiorum lineages in sloths. Genes involved in mobilome and defense mechanisms against mobile genetic elements were the main source of gene content variation between isolates from different hosts. Sloth-specific K. gyiorum genome features include an IncN2 plasmid, a phage sequence, and a CRISPR-Cas system. The broad diversity of defense elements in K. gyiorum (14 systems) may prevent further mobile element flow and explain the low amount of mobile genetic elements in K. gyiorum genomes. Gene content variation may be important for the adaptation of K. gyiorum to different host niches. This study furthers our understanding of diversity, host adaptation, and evolution of K. gyiorum , by presenting and analyzing the first genomes of non-human isolates.
Competing Interests: João C. Setubal is an Academic Editor for PeerJ.
(©2024 Carhuaricra-Huaman et al.)
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فهرسة مساهمة: Keywords: Brown-throated sloths; CRISPR-Cas; Genomics; Host-specific genes; IncN2 plasmid; Kerstersia gyiorum; Mobile genetic elements; Pangenome; Phylogenomics
SCR Organism: Kerstersia gyiorum
تواريخ الأحداث: Date Created: 20240408 Date Completed: 20240409 Latest Revision: 20240409
رمز التحديث: 20240409
مُعرف محوري في PubMed: PMC10999152
DOI: 10.7717/peerj.17206
PMID: 38584940
قاعدة البيانات: MEDLINE
الوصف
تدمد:2167-8359
DOI:10.7717/peerj.17206