التفاصيل البيبلوغرافية
| العنوان: |
Somatic polyploidy supports biosynthesis and tissue function by increasing transcriptional output. |
| المؤلفون: |
Lessenger AT, Swaffer MP, Skotheim JM, Feldman JL |
| المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2024 Mar 27. Date of Electronic Publication: 2024 Mar 27. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
Cell size and biosynthetic capacity generally increase with increased DNA content. Polyploidy has therefore been proposed to be an adaptive strategy to increase cell size in specialized tissues with high biosynthetic demands. However, if and how DNA concentration limits cellular biosynthesis in vivo is not well understood, and the impacts of polyploidy in non-disease states is not well studied. Here, we show that polyploidy in the C. elegans intestine is critical for cell growth and yolk biosynthesis, a central role of this organ. Artificially lowering the DNA/cytoplasm ratio by reducing polyploidization in the intestine gave rise to smaller cells with more dilute mRNA. Highly-expressed transcripts were more sensitive to this mRNA dilution, whereas lowly-expressed genes were partially compensated - in part by loading more RNA Polymerase II on the remaining genomes. DNA-dilute cells had normal total protein concentration, which we propose is achieved by increasing production of translational machinery at the expense of specialized, cell-type specific proteins. |
| معلومات مُعتمدة: |
R35 GM134858 United States GM NIGMS NIH HHS |
| تواريخ الأحداث: |
Date Created: 20240408 Latest Revision: 20240426 |
| رمز التحديث: |
20240426 |
| مُعرف محوري في PubMed: |
PMC10996643 |
| DOI: |
10.1101/2024.03.25.586714 |
| PMID: |
38585999 |
| قاعدة البيانات: |
MEDLINE |
